Real-time visualization of "small Molecules-ssDNA" complexes for aptamer screening based on online competition CE-SELEX.

Capillary electrophoresis-based systematic evolution of ligands by exponential enrichment (CE-SELEX) is one of the most efficient techniques for aptamers screening. However, CE-SELEX is generally considered challenging for small molecule targets due to a slight charge-to-mass ratio (z/m) difference that fails to create a clear separation between small molecule-ssDNA complexes and ssDNA libraries, thus heavily restricting the screening target scopes. Herein, a novel online competition CE-SELEX (ocCE-SELEX) strategy was introduced for real-time visualization of "small molecules-ssDNA" complexes to screen aptamers for small molecule targets in free solution.

Time-resolved transcriptomic and proteomic profiling of during NaOH-buffered L-lactic acid production.

The L-lactic acid (L-LA) fermentation process, based on sodium hydroxide neutralization, demonstrates environmental friendliness during product extraction. However, lactate fermentation is hindered by the pronounced stress effect of sodium lactate on the strain compared with calcium lactate. In this study, we performed time-resolved transcriptomic and proteomic analyses of DSM1 during NaOH-buffered L-LA production.

Identification of a major facilitator superfamily protein that is beneficial to L-lactic acid production by Bacillus coagulans at low pH.

Background: Product inhibition is one of the major problems in lactic acid (LA) fermentation. Our previous study revealed that Bacillus coagulans 2-6 was an efficient producer of high-optical-purity L-LA. Its mutant strain B.

[Annual review of capillary electrophoresis technology in 2020].

This paper reviews the developments in capillary electrophoresis (CE) in 2020. A total of 222 research papers related to CE technology published in 2020 were retrieved from the ISI Web of Science. These papers were selected by using the keywords "capillary electrophoresis-mass spectrometry", "capillary isoelectric focusing", "micellar electrokinetic chromatography", or "capillary electrophoresis" as search conditions.

[Annual review of capillary electrophoresis technology in 2019].

This paper reviews the capillary electrophoresis (CE) in 2019. The literatures searched from ISI Web of Science published in 2019 (Jan.1st to Dec.

Enhanced ε-Poly-L-Lysine Production by the Synergistic Effect of ε-Poly-L-Lysine Synthetase Overexpression and Citrate in .

ε-Poly-L-lysine (ε-PL) is a natural amino acid polymer produced by microbial fermentation. It has been mainly used as a preservative in the food and cosmetics industries, as a drug carrier in medicines, and as a gene carrier in gene therapy. ε-PL synthase is the key enzyme responsible for the polymerization of L-lysine to form ε-PL.

Medical image segmentation based on a hybrid region-based active contour model.

A novel hybrid region-based active contour model is presented to segment medical images with intensity inhomogeneity. The energy functional for the proposed model consists of three weighted terms: global term, local term, and regularization term. The total energy is incorporated into a level set formulation with a level set regularization term, from which a curve evolution equation is derived for energy minimization.