Reduced alpha4beta1 integrin/VCAM-1 interactions lead to impaired pre-B cell repopulation in alpha 1,6-fucosyltransferase deficient mice.

Mice with a targeted gene disruption of Fut8 (Fut8(-/-)) showed an abnormality in the transition from pro-B cell to pre-B cell, reduced peripheral B cells, and a decreased immunoglobulin production. Alpha 1,6-fucosyltransferase (FUT8) is responsible for the alpha 1,6 core fucosylation of N-glycans, which could modify the functions of glycoproteins. The loss of a core fucose in both very late antigen 4 (VLA-4, alpha4beta1 integrin) and vascular cell adhesion molecule 1 (VCAM-1) led to a decreased binding between pre-B cells and stromal cells, which impaired pre-B cells generation in Fut8(-/-) mice.

From glycomics to functional glycomics of sugar chains: Identification of target proteins with functional changes using gene targeting mice and knock down cells of FUT8 as examples.

Comprehensive analyses of proteins from cells and tissues are the most effective means of elucidating the expression patterns of individual disease-related proteins. On the other hand, the simultaneous separation and characterization of proteins by 1-DE or 2-DE followed by MS analysis are one of the fundamental approaches to proteomic analysis. However, these analyses do not permit the complete structural identification of glycans in glycoproteins or their structural characterization.

[Studies on the effect of extracts of several Chinese herbal medicines and other medicines on alcohol dehydrogenase activity].

Objective: To study the effects of water and alcohol extracts of several Chinese herbal medicines and other medicines on alcohol dehydrogenase activity in order to provide enzymology basis on new medicine.

Methods: Water or alcohol extracts of Chinese herbal medicine and other medicine were tested on the effects of alcohol dehydrogenase activity by Valle and Hoch method.

Results: Among them, 8 were found to have the effect of activation on alcohol dehydrogenase.

Down-regulation of trypsinogen expression is associated with growth retardation in alpha1,6-fucosyltransferase-deficient mice: attenuation of proteinase-activated receptor 2 activity.

Alpha1,6-fucosyltransferase (Fut8) plays important roles in physiological and pathological conditions. Fut8-deficient (Fut8-/-) mice exhibit growth retardation, earlier postnatal death, and emphysema-like phenotype. To investigate the underlying molecular mechanism by which growth retardation occurs, we examined the mRNA expression levels of Fut8-/- embryos (18.

Dysregulation of TGF-beta1 receptor activation leads to abnormal lung development and emphysema-like phenotype in core fucose-deficient mice.

The core fucosylation (alpha1,6-fucosylation) of glycoproteins is widely distributed in mammalian tissues, and is altered under pathological conditions. To investigate physiological functions of the core fucose, we generated alpha1,6-fucosyltransferase (Fut8)-null mice and found that disruption of Fut8 induces severe growth retardation and death during postnatal development. Histopathological analysis revealed that Fut8(-/-) mice showed emphysema-like changes in the lung, verified by a physiological compliance analysis.

Suppression of Salmonella enterica serovar Enteritidis excretion by intraocular vaccination with fimbriae proteins incorporated in liposomes.

Liposome-associated fimbriae antigens (SEF14 and SEF21) were prepared for intraocular immunization to seek protective efficacy for intestinal infection with Salmonella enterica serovar Enteritidis. Chickens were immunized intraocularly with the antigens at 8 and 10 weeks of age. Evidence of an IgA and IgG responses were found in the intestinal tract and in sera of these chickens.

[Study on the chemical activity of nicotine by pulse radiolysis].

Nicotine has been studied for the first time by pulse radiolysis techniques. It has been found that hydrated electron, hydrogen radical, and hydroxyl radical can react on nicotine to produce an anion radical and neutral radical, respectively. Different absorption spectra were obtained, and the related rate constants were determined.

Identification of glycosphingolipid binding sites for SEF21-fimbriated Salmonella enterica serovar Enteritidis in chicken oviductal mucosa.

In order to clarify the presence of glycosphingolipids (GSLs) receptors for Salmonella enterica serovar Enteritidis with SEF21 fimbriae, we analyzed neutral GSLs and gangliosides from chicken oviductal mucosa and investigated the binding of bacteria to neutral GSLs and gangliosides. Five types of neutral GSLs, designated as N-1 to N-5, and two types of gangliosides, designated as G-1 and G-2, were identified on the thin-layer chromatography (TLC) plates. In the bacterial binding assay on TLC, the fimbriated bacteria bound only to glucosylceramide (GlcCer) standard, N-1 having the same TLC mobility as GlcCer, GM3 standard and G-1 corresponding to GM3 in TLC mobility, but not to N-2, N-3, N-4, N-5, or G-2.

Identification of possible chicken intestinal mucosa receptors for SEF21-fimbriated Salmonella enterica serovar Enteritidis.

In order to test whether glycosphingolipids (GSLs) on the chicken intestinal mucosa serve as a receptor for Salmonella enterica serovar Enteritidis with fimbriae, we analyzed neutral GSLs and gangliosides from chicken intestinal mucosa and investigated the binding of bacteria to neutral GSLs and gangliosides. Four kinds of neutral GSLs, designated as N-1 to N-4 and four kinds of gangliosides, named G-1 to G-4, were identified on high-performance thin-layer chromatography (HPTLC) plates. In TLC immunostaining tests, fimbriated S.