Au@bovine serum albumin nanoparticle-based acid-resistant nanozyme quartz crystal microbalance sensing of urine glucose.

A robust, efficient and sensitive quartz crystal microbalance (QCM) for glucose detection has been constructed using Au@bovine serum albumin (Au@BSA) nanoparticles as an active layer. The nanoparticles serve as tandem nanozymes and their stability over natural enzymes enable the sensor to show a wider linear dynamic range between 0.05 and 15 mM, a higher acid-resistance (pH 2.

[Expression of receptor-binding cancer antigen expressed on SiSo cells in endometrial carcinoma and the correlation thereof with the expression of estrogen receptor subtypes].

Objective: To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) and estrogen receptor subtypes in normal, hyperplastic, and carcinomatous endometrium and to explore their possible roles in carcinogenesis and progression of endometrial carcinoma.

Methods: Immunohistochemistry and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) were applied to detect the protein and mRNA expression of RCAS1, estrogen receptor (ER) alpha and ERbeta in 20 specimens of normal endometrium, 24 specimens of hyperplastic endometrium, and 50 specimens of carcinomatous endometrium. Western blotting was used to detect the RCAS1 protein expression.