A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection.

The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy is demonstrated using the small molecule biotin and its receptor protein streptavidin (SA).

MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy.

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed.

Atorvastatin alleviates microglia-mediated neuroinflammation via modulating the microbial composition and the intestinal barrier function in ischemic stroke mice.

Our previous work has shown that atorvastatin exerts anti-inflammatory properties in ischemic stroke, and recent studies have revealed that intestinal microbiota plays a vital role in the pathogenesis of stroke. However, it is not clear whether the anti-inflammatory effects of atorvastatin against ischemic stroke is related to gut function and microbiota. We report herein that atorvastatin significantly ameliorated the defects in sensorimotor behaviors and reduced microglia-mediated neuroinflammation by inhibiting proinflammatory polarization of microglia in the peri-infarct cortex of the mice with permanent middle cerebral artery occlusion (pMCAO).

Ginsenoside Rg1 promotes cerebral angiogenesis via the PI3K/Akt/mTOR signaling pathway in ischemic mice.

Angiogenesis plays an important role in the remodeling process of the ischemic brain and the recovery of neurological function after ischemic stroke. Ginsenoside Rg1 has been reported to exert neuroprotective effects on the central nervous system. However, the effects of ginsenoside Rg1 on cerebral angiogenesis in cerebral ischemia remained unclear.

A Duplex PCR Assay for Rapid Detection of and .

A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens and . The specific primers for were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, gene and gene, while the specific primers for were designed based on its ITS regions and tubulin gene. The specificity of the primers was determined using isolates of , and control samples.

[Phosphorus and sulphur bio-cycling in alpine tundra ecosystem of Changbai Mountains].

The study with compartment model on the phosphorus (P) and sulphur (S) bio-cycling in alpine tundra ecosystem of Changbai Mountains showed that the total storage of P and S was 16 088.6 t and 26 079.4 t, of which, 46.

[Nitrogen bio-cycle in the alpine tundra ecosystem of Changbai Mountain and its comparison with arctic tundra].

The nitrogen bio-cycle was discussed in the alpine tundra ecosystem of Changbai Mountain through compartment model. The alpine tundra of Changbai Mountain was compared with Arctic tundra by the common ratio of genus and species in this paper. It was found that the 89.

[Maintaining mechanism of species diversity of land plant communities].

The maintaining mechanism of species diversity of land plant communities is a key and advancing edge in biodiversity study. Botanists and ecologists have presented many hypotheses and theories with controversies, and no general theory system was available. In this paper, the problem was reviewed mainly on two scales.