N-methyladenosine modification-mediated mRNA metabolism is essential for human pancreatic lineage specification and islet organogenesis.

Pancreatic differentiation from human pluripotent stem cells (hPSCs) provides promising avenues for investigating development and treating diseases. N-methyladenosine (mA) is the most prevalent internal messenger RNA (mRNA) modification and plays pivotal roles in regulation of mRNA metabolism, while its functions remain elusive. Here, we profile the dynamic landscapes of mA transcriptome-wide during pancreatic differentiation.

Inhibition of Syk promotes chemical reprogramming of fibroblasts via metabolic rewiring and H S production.

Chemical compounds have recently been introduced as alternative and non-integrating inducers of pluripotent stem cell fate. However, chemical reprogramming is hampered by low efficiency and the molecular mechanisms remain poorly characterized. Here, we show that inhibition of spleen tyrosine kinase (Syk) by R406 significantly promotes mouse chemical reprogramming.

Quantifying the contributions of human activities and climate change to vegetation net primary productivity dynamics in China from 2001 to 2016.

Vegetation is an important component of the terrestrial ecosystem, driven by climate change and human activities. Quantifying the relative contributions of climate change and anthropogenic activities to vegetation dynamics are essential to cope with global climate change. In this paper, the relative contributions of anthropogenic activities and climate change to net primary productivity (NPP) in China were analyzed by a two-step methodology based on the residual trend analysis (RESTREND).

Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells.

Human pluripotent stem cells (hPSCs) have potential applications in biological studies and regenerative medicine. However, precise genome editing in hPSCs remains time-consuming and labor-intensive. Here we demonstrate that the recently identified CRISPR-Cpf1 can be used to efficiently generate knockout and knockin hPSC lines.