System analysis identifies UBE2C as a novel oncogene target for adrenocortical carcinoma.

Ubiquitin Conjugating Enzyme 2C (UBE2C) is an emerging target gene for tumor progression. However, the tumorigenic effect and mechanism of UBE2C in adrenocortical carcinoma (ACC) remains unclear. Systematic investigation of the tumorigenic effect of UBE2C may help in understanding its prognostic value in adrenocortical carcinoma.

Risk Assessment and Prevention of Severe Acute Respiratory Syndrome Coronavirus 2 Transmission for Hospitalized Urological Patients After the COVID-19 Pandemic in Wuhan, China.

Background: Emerging asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections were detected and multiple cases were found to be SARS-CoV-2 positive again, which raised an alarm for the patients hospitalized after the coronavirus disease 2019 (COVID-19) pandemic.

Objective: We investigated the risk and prevention of hospital transmission of SARS-CoV-2 to hospitalized urological patients.

Design Setting And Participants: This is a retrospective study of 319 hospitalized urological patients enrolled between April 20, 2020 and May 11, 2020 from two tertiary hospitals in Wuhan, China.

Expression of recombinant protein rSjE16 and its effects on LX-2 cells .

Objective: The activation of hepatic stellate cells (HSCs) is a key event in schistosome-induced liver fibrosis. Previous studies have shown that soluble egg antigens and the recombinant P40 protein from eggs inhibit HSC activation. In the present study, we observed the direct effect of the recombinant (r)SjE16 protein on HSCs.

Factors associated with neonatal resuscitation knowledge and comfort across academic anesthesia institutions.

Background: Neonatal resuscitation training is a requirement for all obstetric anesthesia fellows. However, while the majority of anesthesiologists who work on labor and delivery report having been involved in the resuscitation of a newborn, most do not have NRP training.

Objective: By studying a national cohort of anesthesiologists, our objective was to identify factors associated with knowledge and comfort with neonatal resuscitation and to inform decisions about neonatal resuscitation in obstetric anesthesia fellowship training.

Magnetically Directed Enzyme/Prodrug Prostate Cancer Therapy Based on β-Glucosidase/Amygdalin.

Background: β-Glucosidase (β-Glu) can activate amygdalin to kill prostate cancer cells, but the poor specificity of this killing effect may cause severe general toxicity in vivo, limiting the practical clinical application of this approach.

Materials And Methods: In this study, starch-coated magnetic nanoparticles (MNPs) were successively conjugated with β-Glu and polyethylene glycol (PEG) by chemical coupling methods. Cell experiments were used to confirm the effects of immobilized β-Glu on amygdalin-mediated prostate cancer cell death in vitro.

Coordination-Directed Construction of Molecular Links.

Since the emergence of the concept of chemical topology, interlocked molecular assemblies have graduated from academic curiosities and poorly defined species to become synthetic realities. Coordination-directed synthesis provides powerful, diverse, and increasingly sophisticated protocols for accessing interlocked molecules. Originally, metal ions were employed solely as templates to gather and position building blocks in entwined or threaded arrangements.

Dynamic Interconversion between Solomon Link and Trapezoidal Metallacycle Ensembles Accompanying Conformational Change of the Linker.

A novel template-free Cp*Rh-based molecular Solomon link has been established through selection of the flexible ligand L as a linker and the half-sandwich rhodium(III) dinuclear fragment B1 as a rigid capping unit. Furthermore, we demonstrate that the self-assembly of the Solomon link based on the flexible ligand is both solvent- and concentration-dependent: the Solomon link is formed in concentrated methanolic solutions, whereas formation of a dinuclear trapezoidal rectangle is favored at low concentrations or in acetonitrile or DMF solutions. Remarkably, alteration of the solvent or concentration can promote a unique and dynamic interconversion between the two molecular species, accompanying conformational change of the ligand.

Reversible Structural Transformation between a Molecular Solomon Link and an Unusual Unsymmetrical Trefoil Knot.

A template-free Cp*Ir-based molecular Solomon link and an unusual trefoil knot induced by stacking interactions were realized via selection of the nonrigid dipyridyl ligand 4,4'-bis(pyridin-4-ylmethyl)-1,1'-biphenyl (). In addition, a novel heterobimetallic tetranuclear (Ir + Ag) complex featuring argentophilic interaction was synthesized in high yield. Remarkably, the reversible topological transformation between the Solomon link and an unusual trefoil knot can be achieved by utilizing the chemical reactivity of silver(I) ions under mild conditions, as demonstrated by a detailed X-ray crystallographic study.

Sanjin tablets for acute uncomplicated lower urinary tract infection (syndrome of dampness-heat in the lower jiao): protocol for randomized, double-blind, double-dummy, parallel control of positive drug, multicenter clinical trial.

Background: Acute uncomplicated lower urinary tract infection (UTI) is one of the most common bacterial infections. Patients usually present with dysuria, urinary urgency, urinary frequency, and suprapubic pain or tenderness. Approximately 150 million people are diagnosed with UTI each year worldwide.

Selective Synthesis of Discrete Mono-, Interlocked-, and Borromean Ring Ensembles Based on a π-Electron-Deficient Ligand.

Herein, we present a new synthetic approach to achieve selective supramolecular transformations and construct different interlocked metallacycles featuring a π-electron-deficient thiazolo[5,4-d]thiazole-derived ligand. We demonstrate that the formation of mono-rings, interlocked rings ([2]catenanes) and Borromean rings can be controlled by adjusting the length of the binuclear half-sandwich Rh and Ir building blocks. Furthermore, a concentration effect or D-A stacking interaction between the pyrene guest and the thiazolo[5,4-d]thiazole-based ligand promotes a unique and reversible conversion between catenane structures and metalla-rectangles.

Covalent Post-assembly Modification Triggers Structural Transformations of Borromean Rings.

A series of supramolecular transformation cascades are presented here, employing ligand exchange reactions, concentration-dependent Borromean ring (BR) linking and unlinking, and inverse electron-demand Diels-Alder (IEDDA) reactions. The new family of template-free, tetrazine-edged BRs are constructed by using ligand exchange reactions, and their concentration-dependent linking and unlinking reactions have been observed. Moreover, Borromean precursors have been demonstrated to further undergo supramolecular structural transformations induced by rapid and efficient IEDDA reactions to afford corresponding post-assembly modified architectures.

Selective Encapsulation and Separation of Dihalobenzene Isomers with Discrete Heterometallic Macrocages.

A series of metallosupramolecular architectures have been prepared, including rectangles, prisms and cages, that feature half-sandwich rhodium(III) fragments at the vertices. Remarkably, a stable cage-like heteropolymetallic complex possessing eight rhodium(III) and two silver(I) metal ions (3) has been obtained following a multistep procedure. The Rh /Ag mixed macrocage enables the separation of dihalogenated benzene derivatives with high selectivity.

Preparation of polynuclear NHC complexes by post-synthetic modification of half-sandwich rhodium and iridium complexes bearing C-azolato ligands.

A series of bimetallic and trimetallic complexes has been prepared by N-alkylation of mononuclear half-sandwich rhodium and iridium di-NHC complexes featuring bidentate chelate ligands composed of a classical NHC and a C-azolato donor. The shape and the separation between metal centers in the obtained complexes are highly dependent on the alkylating agent used. While the polynuclear complexes were formed as diastereomeric mixtures according to NMR spectroscopy, X-ray diffraction analysis revealed their remarkable preference to crystallize as meso compounds.

Construction of half-sandwich multinuclear complexes including tunnel architectures via C-H-activation-directed assembly.

Three aromatic ligands containing carboxyl- or nitrogen-substituted groups were employed in the construction of half-sandwich complexes via C-H activation-directed assembly, leading to the construction of eleven multinuclear organometallic iridium or rhodium complexes, including those of bi-, tetra-, hexa- and octanuclear, under mild conditions, with the help of N-donor linkers. These complexes were characterized by proton NMR, IR spectroscopy, elemental analysis, electrospray ionization (ESI) mass spectrometry and single-crystal X-ray diffraction analysis. In these complexes, two complexes were observed to exist as isomers, and several racemic enantiomers can be found.

MicroRNA-1180 is associated with growth and apoptosis in prostate cancer via TNF receptor associated factor 1 expression regulation and nuclear factor-κB signaling pathway activation.

In the present study, the aim was to investigate the role of microRNA-1180 (miR-1180) in the growth and apoptosis of prostate cancer, as well as to identify its direct targets. Initially, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to examine the expression of miR-1180 in the prostate cancer tissues and adjacent normal prostate tissues of 30 patients, as well as in DU145 and RWPE-1 cells. Next, DU145 cells were transfected with miR-1180 mimics, and the expression levels of associated proteins were determined by western blot assay.

Light-initiated reversible conversion of macrocyclic endoperoxides derived from half-sandwich rhodium-based metallarectangles.

Although reversible photo-dimerization or oxygenation of anthracene and its derivatives is a common reaction, light-initiated reversible conversion of endoperoxide organometallic frameworks has only rarely been addressed. Herein, a series of tetranuclear organometallic macrocycles, [Cp*Rh(μ-CO-κO)](BP4VA)(OTf) (4), [Cp*Rh(BiBzIm)](BP4VA)(OTf) (5), and [Cp*Rh(DHBQ)](BP4VA)(OTf) (6), were obtained in good yields from the reactions of the binuclear half-sandwich rhodium precursors [Cp*Rh(μ-CO-κO)Cl] (1), [Cp*Rh(BiBzIm)Cl] (2), and [Cp*Rh(DHBQ)Cl] (3) with the 9,10-bis((E)-2-(pyrid-4-yl)vinyl)anthracene (BP4VA) ligand. The photochemical reaction of these metallarectangles was investigated by NMR and UV/vis spectroscopy.

Stacking-interaction-induced host-guest chemistry and Borromean rings based on a polypyridyl ligand.

Template-free molecular Borromean rings (BRs) and open-ended molecular capsules were realized by strategic selection of the polypyridyl ligand tetrapyrido[3,2-a:2',3'-c:3'',2''-h:2''',3'''-j] phenazine (TPPHZ) with a large surface area and specific geometry. The topology of these intricate architectures was constructed via precisely controlled stacking interactions, as evidenced from single-crystal X-ray analysis.

How to accelerate the upper urinary stone discharge after extracorporeal shockwave lithotripsy (ESWL) for < 15 mm upper urinary stones: a prospective multi-center randomized controlled trial about external physical vibration lithecbole (EPVL).

Objective: To asset the efficacy and safety of EPVL plus ESWL compared with ESWL alone for the treatment of simple upper urinary stones (< 15 mm).

Materials And Methods: All patients with upper urinary stones (< 15 mm) were prospectively randomized into two groups. In treatment group, patients were assigned to immediate EPVL after ESWL, while in control group, ESWL alone was offered.

Efficacy and Safety of Tamsulosin in Medical Expulsive Therapy for Distal Ureteral Stones with Renal Colic: A Multicenter, Randomized, Double-blind, Placebo-controlled Trial.

Background: Recent large high-quality trials have questioned the clinical effectiveness of medical expulsive therapy using tamsulosin for ureteral stones.

Objective: To evaluate the efficacy and safety of tamsulosin for distal ureteral stones compared with placebo.

Design, Setting, And Participants: We conducted a double-blind, placebo-controlled study of 3296 patients with distal ureteral stones, across 30 centers, to evaluate the efficacy and safety of tamsulosin.

Stepwise construction of discrete parallelogram- and prism-shaped organometallic architectures based on half-sandwich rhodium fragments.

Herein we describe how to take advantage of a multifunctional ligand to conveniently control the shapes and sizes of different types of discrete organometallic assemblies. Using a versatile ligand 2-(pyridin-2-yl)-1H-imidazole-4,5-dicarboxylate (HL), which features three kinds of chelating sites, together with half-sandwich rhodium fragments, a series of discrete organometallic complexes with tetranuclear parallelogram and hexanuclear trigonal prism shapes were achieved. The isomerization of the hexanuclear assemblies was confirmed by single-crystal X-ray diffraction analysis.

Syntheses, Structures, and Solution Studies of Multicomponent Macrocycles and Cages Based on Versatile Ligands.

Different types of multinuclear half-sandwich rhodium macrocycles and cages were designed and synthesized by using two similar multifunctional hydroxamate ligands (pyrazine-2-hydroxamic acid (NaHL ) and 4,4'-bipyridine-2-hydroxamic acid (KHL )) featuring one monodentate site and two pairs of chelating sites. The Rh -Pd heterometallic macrocycles were constructed by using the semi-open palladium(II) source [Pd(en)Cl ] with two free acceptor sites. However, only one kind of macrocycle was found when the shorter ligand L was used, while in for the larger ligand, various spectroscopic techniques demonstrated the coexistence of hexanuclear and octanuclear macrocycles in solution and the proportions of both components depended on concentration and temperature.

External Physical Vibration Lithecbole Promotes the Clearance of Upper Urinary Stones after Retrograde Intrarenal Surgery: A Prospective, Multicenter, Randomized Controlled Trial.

Purpose: We assessed the efficacy and safety of external physical vibration lithecbole for the treatment of residual stones after retrograde intrarenal surgery.

Materials And Methods: A total of 173 patients (128 males and 45 females) were selected for study. All patients had residual fragments after retrograde intrarenal surgery for renal or upper ureteral stones.

Enhanced and selective delivery of enzyme therapy to 9L-glioma tumor via magnetic targeting of PEG-modified, β-glucosidase-conjugated iron oxide nanoparticles.

The stability of enzyme-conjugated magnetic iron oxide nanoparticles in plasma is of great importance for in vivo delivery of the conjugated enzyme. In this study, β-glucosidase was conjugated on aminated magnetic iron oxide nanoparticles using the glutaraldehyde method (β-Glu-MNP), and further PEGylated via N-hydroxysuccinimide chemistry. The PEG-modified, β-glucosidase-immobilized magnetic iron oxide nanoparticles (PEG-β-Glu-MNPs) were characterized by hydrodynamic diameter distribution, zeta potential, Fourier transform infrared spectroscopy, transmission electron microscopy, and a superconducting quantum interference device.

α1-Antitrypsin inhibits ischemia reperfusion-induced lung injury by reducing inflammatory response and cell death.

Background: Pulmonary ischemia-reperfusion (IR)-induced lung injury is a severe complication that increases the likelihood of primary graft dysfunction and early death after lung transplantation. Inflammatory cytokine release and cell death play a critical role in the development of IR-induced lung injury. α1-Antitrypsin (A1AT) is a protease inhibitor clinically used for the treatment of A1AT-deficiency emphysema.