A Potent Antibacterial Peptide (P6) from the De Novo Transcriptome of the Microalga .

Marine microalgae are a rich source of natural products, and their amino acid-based antimicrobial agents are usually obtained by enzymatic hydrolysis, which is inefficient and limits the research on antimicrobial peptides (AMPs) from microalgae. In this study, is used as a model to predict antimicrobial peptides through high-throughput methods, and 471 putative peptides are identified based on the de novo transcriptome technique. Among them, three short peptides, P1, P6, and P7 were found to have antimicrobial activity against , , , and yeast , and they showed no hemolytic activity even at higher concentrations up to 10 mg/mL.