[BMP9 promotes osteogenic differentiation of periodontal ligament stem cells under inflammatory environment via ERK5/KLF4 signaling pathway].

Purpose: To explore how bone morphogenetic protein 9(BMP9) promotes the osteogenic differentiation of periodontal ligament stem cells(PDLSCs) via ERK5/KLF4 signaling pathway under an inflammatory environment.

Methods: Recombinant adenovirus to overexpress BMP9 in PDLSCs stimulated with TNF-α was used and the expression level of osteogenic-related genes and proteins in BMP9-treated cells was examined using RT-PCR and Western blot. Then, KLF4 was overexpressed in PDLSCs via transfection and subjected to inflammatory stimulation to observe its effects on osteogenic differentiation and the expression of osteogenic-related genes.

The mechanism of China's renewable energy utilization impact on carbon emission intensity: Evidence from the perspective of intermediary transmission.

Renewable energy (RE) plays a crucial role in global energy transformation, and a thorough study of the potential impact of RE on regional carbon emissions is of great significance. This is particularly relevant to China, which needs to clarify its path to carbon reduction. Using the sample data of 30 provinces in China from 2000 to 2021, this paper uses the Granger causality test to verify the causal relationship between carbon emission intensity (CEI) and other factors.

Regional differences and driving factors of carbon emission intensity in China's electricity generation sector.

As an industry with immense decarbonization potential, the low-carbon transformation of the power sector is crucial to China's carbon emission (CE) reduction commitment. Based on panel data of 30 provinces in China from 2000 to 2019, this research calculates and analyzes the provincial CE intensity in electricity generation (CEIE) and its spatial distribution characteristics. Additionally, the GTWR model based on the construction explains the regional heterogeneity and dynamic development trend of each driving factor's influence on CEIE from time and space.

Coordinated development and driving factor heterogeneity of different types of urban agglomeration carbon emissions in China.

Carbon emission (CE) reduction has become the primary task of China's urban agglomerations (UAs) in achieving sustainable development goals. This paper uses a decoupling model and coupling coordination model to measure the relationship between the development levels of different types of UAs and CEs in China from 2004 to 2016. Concurrently, the geographically and temporally weighted regression model is used to explore the spatial heterogeneity of the impact of different driving factors on the CEs of UAs.

DNCP induces the differentiation of induced pluripotent stem cells into odontoblasts by activating the Smad/p-Smad and p38/p-p38 signaling pathways.

In recent years, stem cells have been studied for treating tooth loss. The present study aimed to investigate the roles of dentin non-collagen protein (DNCP)-associated microenvironments in the differentiation of induced pluripotent stem cells (iPSCs) into dentin cells. iPSCs were cultured and identified by examining octamer-binding transcription-factor-4 (Oct-4) and sex-determining region-Y-2 (Sox-2) expression.

Application of a new drainage plug for large mandibular cysts after fenestration decompression.

Background: In order to improve the postoperative decompression and drainage of large mandibular cysts after fenestration decompression, a new drainage plug was designed and its feasibility for clinical application was explored.

Methods: A total of 74 patients with large mandibular cysts requiring fenestration decompression were included and randomly divided into the control group (n=34) and model group (n=40). Patients in the control group were given a conventional plug, while patients in the model group were given the new silicone drainage plug.

STAT3-induced upregulation of long noncoding RNA HNF1A-AS1 promotes the progression of oral squamous cell carcinoma via activating Notch signaling pathway.

Long non-coding RNAs (lncRNAs) are a group of biomarkers which can regulate the biological processes of various human cancers. LncRNA HNF1A-AS1 has been reported in human cancers for its oncogenic role. This study focused on the biological function and molecular mechanism of HNF1A-AS1 in oral squamous cell carcinoma (OSCC).

Establishment of a congenital tooth agenesis related gene MSX1 knockout human embryonic stem cell lines by CRISPR-Cas9 technology.

Human MSX1 gene is mapped to chromosome 4 and encodes a 303aa homeobox protein MSX1. MSX1 expression appears during early tooth development of vertebrate embryogenesis. Mutations in this protein are related to human tooth anomalie, cleft lip and palate and congenital ectodermal dysplasia syndrome.