Genome Analysis of a Polysaccharide-Degrading Bacterium sp. HZ11 and Degradation of Alginate.

Marine bacteria are crucial sources of alginate lyases, which play an essential role in alginate oligosaccharide (AOS) production. This study reports the biochemical characteristics of a new species of the genus, sp. HZ11.

Convolutional neural network-assisted Raman spectroscopy for high-precision diagnosis of glioblastoma.

Glioblastoma multiforme (GBM) is the most lethal intracranial tumor with a median survival of approximately 15 months. Due to its highly invasive properties, it is particularly difficult to accurately identify the tumor margins intraoperatively. The current gold standard for diagnosing GBM during surgery is pathology, but it is time-consuming.

Multi-View Fusion-Based Automated Full-Posture Cattle Body Size Measurement.

Cattle farming is an important part of the global livestock industry, and cattle body size is the key indicator of livestock growth. However, traditional manual methods for measuring body sizes are not only time-consuming and labor-intensive but also incur significant costs. Meanwhile, automatic measurement techniques are prone to being affected by environmental conditions and the standing postures of livestock.

Realization of quasi-steady-state piled smouldering using sequential operation chambers for industrial treatment of biomass wastes.

Piled smouldering has great potential for treatment and utilization of biomass wastes. However, its unsteady-state nature limits its industrial utilization, as well as treatment of smoke. This article addresses this issue by proposing the sequential operation of numerous smouldering chambers to realize steady- or quasi-steady-state piled smouldering.

Ketogenic β-hydroxybutyrate regulates β-hydroxybutyrylation of TCA cycle-associated enzymes and attenuates disease-associated pathologies in Alzheimer's mice.

Lysine β-hydroxybutyrylation (Kbhb) is a post-translational modification that has recently been found to regulate protein functions. However, whether and how protein Kbhb modification participates in Alzheimer's disease (AD) remains unknown. Herein, we carried out 4D label-free β-hydroxybutylation quantitative proteomics using brain samples of 8-month-old and 2-month-old APP/PS1 AD model mice and wild-type (WT) controls.

The proto-oncogene tyrosine kinase c-SRC facilitates glioblastoma progression by remodeling fatty acid synthesis.

Increased fatty acid synthesis benefits glioblastoma malignancy. However, the coordinated regulation of cytosolic acetyl-CoA production, the exclusive substrate for fatty acid synthesis, remains unclear. Here, we show that proto-oncogene tyrosine kinase c-SRC is activated in glioblastoma and remodels cytosolic acetyl-CoA production for fatty acid synthesis.

Immediate and long-term efficacy of transcranial direct current stimulation (tCDS) in obsessive-compulsive disorder, posttraumatic stress disorder and anxiety disorders: a systematic review and meta-analysis.

Currently, there is still debate over the effectiveness of transcranial direct current stimulation (tDCS) in treating obsessive-compulsive disorder (OCD), posttraumatic stress disorder (PTSD) and anxiety disorders (ADs). To investigate the immediate and long-term effectiveness of tDCS in these diseases, we conducted a systematic review and quantitative analysis of existing literature on the treatment of OCD, PTSD, and ADs with tDCS. Following the PRISMA guidelines, we searched seven electronic databases and systematically retrieved articles published from May 2012 to June 2024 that compared the effects of active tDCS with sham stimulation in the treatment of these disorders.

Fast prediction of personalized abdominal organ doses from CT examinations by radiomics feature-based machine learning models.

The X-rays emitted during CT scans can increase solid cancer risks by damaging DNA, with the risk tied to patient-specific organ doses. This study aims to establish a new method to predict patient specific abdominal organ doses from CT examinations using minimized computational resources at a fast speed. The CT data of 247 abdominal patients were selected and exported to the auto-segmentation software named DeepViewer to generate abdominal regions of interest (ROIs).

Carvacrol ameliorates skin allograft rejection through modulating macrophage polarization by activating the Wnt signalling pathway.

Post-transplantation immune rejection remains an important factor for transplant patients. However, conventional immunosuppressants are associated with substantial adverse effects. Natural immunosuppressants present a promising alternative to conventional counterparts, boasting exceptional biological activity, minimal toxicity and reduced side effects.

The combination of temozolomide and perifosine synergistically inhibit glioblastoma by impeding DNA repair and inducing apoptosis.

Temozolomide (TMZ) is widely utilized as the primary chemotherapeutic intervention for glioblastoma. However, the clinical use of TMZ is limited by its various side effects and resistance to chemotherapy. The present study revealed the synergistic inhibition of glioblastoma through the combined administration of TMZ and perifosine.

Evaluation of the mechanistic basis for the antibacterial activity of ursolic acid against .

The antibiotics are generally regarded as the first choice approach to treat dairy mastitis, targeting the public health problems associated with the food safety and the emergence of antibioticresistant bacteria. The objective of the study was to evaluate the antibacterial efficacy of ursolic acid (UA) when used to treat and other isolates associated with bovine mastitis and to clarify the mechanistic basis for these effects. The bacteriostatic properties of UA extracted from L.

ChemFH: an integrated tool for screening frequent false positives in chemical biology and drug discovery.

High-throughput screening rapidly tests an extensive array of chemical compounds to identify hit compounds for specific biological targets in drug discovery. However, false-positive results disrupt hit compound screening, leading to wastage of time and resources. To address this, we propose ChemFH, an integrated online platform facilitating rapid virtual evaluation of potential false positives, including colloidal aggregators, spectroscopic interference compounds, firefly luciferase inhibitors, chemical reactive compounds, promiscuous compounds, and other assay interferences.

Hepatocyte-specific METTL3 ablation by Alb-iCre mice (GPT), but not by Alb-Cre mice (JAX), resulted in acute liver failure (ALF) and postnatal lethality.

Aim: In 2019, to examine the functions of METTL3 in liver and underlying mechanisms, we generated mice with hepatocyte-specific METTL3 homozygous knockout (METTL3Δhep) by simultaneously crossing METTL3fl/fl mice with Alb-iCre mice (GPT) or Alb-Cre mice (JAX), respectively. In this study, we explored the potential reasons why hepatocyte-specific METTL3 homozygous disruption by Alb-iCre mice (GPT), but not by Alb-Cre mice (JAX), resulted in acute liver failure (ALF) and then postnatal lethality.

Main Methods: Mice with hepatocyte-specific METTL3 knockout were generated by simultaneously crossing METTL3fl/fl mice with Alb-iCre mice (GPT; Strain No.

ADMETlab 3.0: an updated comprehensive online ADMET prediction platform enhanced with broader coverage, improved performance, API functionality and decision support.

ADMETlab 3.0 is the second updated version of the web server that provides a comprehensive and efficient platform for evaluating ADMET-related parameters as well as physicochemical properties and medicinal chemistry characteristics involved in the drug discovery process. This new release addresses the limitations of the previous version and offers broader coverage, improved performance, API functionality, and decision support.

Targeting the "tumor microenvironment": RNA-binding proteins in the spotlight in colorectal cancer therapy.

Colorectal cancer (CRC) is the third most common cancer and has the second highest mortality rate among cancers. The development of CRC involves both genetic and epigenetic abnormalities, and recent research has focused on exploring the ex-transcriptome, particularly post-transcriptional modifications. RNA-binding proteins (RBPs) are emerging epigenetic regulators that play crucial roles in post-transcriptional events.

Microplastics in different municipal solid waste treatment and disposal systems: Do they pose environmental risks?

Microplastic (MP) pollution is a significant worldwide environmental and health challenge. Municipal solid waste (MSW) can be an important source of MPs in the environment if treated and disposed of inappropriately, causing potential ecological risks. MSW treatment and disposal methods have been gradually shifting from landfilling/dumping to more sustainable approaches, such as incineration or composting.

Neuroticism and openness exhibit an anti-correlation pattern to dissociable default mode network: using resting connectivity and structural equation modeling analysis.

The default mode network (DMN) can be subdivided into ventral and dorsal subsystems, which serve affective cognition and mental sense construction, respectively. An internally dissociated pattern of anti-correlations was observed between these two subsystems. Although numerous studies on neuroticism and openness have demonstrated the neurological functions of the DMN, little is known about whether different subsystems and hubs regions within the network are engaged in different functions in response to the two traits.

ChemMORT: an automatic ADMET optimization platform using deep learning and multi-objective particle swarm optimization.

Drug discovery and development constitute a laborious and costly undertaking. The success of a drug hinges not only good efficacy but also acceptable absorption, distribution, metabolism, elimination, and toxicity (ADMET) properties. Overall, up to 50% of drug development failures have been contributed from undesirable ADMET profiles.

Plasma levels of hydrogen sulfide and homocysteine correlate with the efficacy of antidepressant agents and serve as potential diagnostic and therapeutic markers.

Objective: Hydrogen sulfide (HS) is associated with depressive-like behavior in rodents. We undertook cross-sectional and longitudinal analyses of plasma levels of HS and its substrate homocysteine (Hcy) in depression and assessed the association of both parameters with psychopathology and cognitive function.

Methods: Forty-one patients suffering from depression (PSDs) and 48 healthy volunteers were recruited.

Occurrence, behaviors, and fate of per- and polyfluoroalkyl substances (PFASs) in typical municipal solid waste disposal sites.

Per- and polyfluoroalkyl substances (PFASs) have become a crucial environmental concern owing to their exceptional persistence, ability to bioaccumulate within ecosystems, and potential to adversely affect biota. Products and materials containing PFASs are usually discarded into municipal solid waste (MSW) at the end of their life cycle, and the fate of PFASs may differ when different disposal methods of MSWs are employed. To date, limited research has focus on the occurrence, behaviors, and fate of PFASs emitted from various MSW disposal sites.