A Highly Selective Fluorescent Probe for Imaging Hydrogen Sulfide in Living HeLa Cells.

As an important endogenous gasotransmitter, hydrogen sulfide (HS) has been implicated with a variety of biological processes and has attracted more and more attention for its key role in a wide range of pathological processes. However, lacking tools for HS-specific in situ detection, the changes of endogenous HS levels in the pathological progression of diseases are still unclear. In this work, a turn-on fluorescent probe (BF-DBS) has been designed and synthesized by two-step reactions using 4-diethylaminosalicylaldehyde and 1,4-dimethylpyridinium iodide as raw materials.

Anti-Seizure and Neuronal Protective Effects of Irisin in Kainic Acid-Induced Chronic Epilepsy Model with Spontaneous Seizures.

An increased level of reactive oxygen species is a key factor in neuronal apoptosis and epileptic seizures. Irisin reportedly attenuates the apoptosis and injury induced by oxidative stress. Therefore, we evaluated the effects of exogenous irisin in a kainic acid (KA)-induced chronic spontaneous epilepsy rat model.

Attenuated succinate accumulation relieves neuronal injury induced by hypoxia in neonatal mice.

Hypoxia causes neonatal neuronal damage. However, the underlying mechanism remains unclear. This study aimed to explore the changes in succinate levels and identify the mechanisms underlying their contribution to hypoxia-induced damage in newborn mice.

Is amyloid binding alcohol dehydrogenase a drug target for treating Alzheimer's disease?

Current strategies for the treatment of Alzheimer's disease (AD) involve tackling the formation or clearance of the amyloid-beta peptide (Aβ) and/or hyper-phosphorylated tau, or the support and stabilization of the remaining neuronal networks. However, as we gain a clearer idea of the large number of molecular mechanisms at work in this disease, it is becoming clearer that the treatment of AD should take a combined approach of dealing with several aspects of the pathology. The concept that we also need to protect specific sensitive targets within the cell should also be considered.

Dibromidobis{1-[4-(pyridin-4-yl)phen-yl]ethanone-κN}mercury(II).

In the title compound, [HgBr(2)(C(13)H(11)NO)(2)], the Hg(II) atom adopts a four-coordinated HgN(2)Br(2) geometry, formed by two pyridine N atoms from two ligands and two bromide anions. The complex is located on a twofold axis. The coordination geometry is close to forming a see-saw (SS-4) polyhedron, the symmetry-related organic ligands being almost perpendicular; the dihedral angles between the two pyridine rings and between the two benzene rings are 85.

Cu2+-selective naked-eye and fluorescent probe: its crystal structure and application in bioimaging.

A new fluorescent and colorimetric Cu2+ probe was synthesized, and realized optical imaging in RAW264.7 macrophages. The design strategy was based on a change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine-based dyes, and its crystal structure was presented to explain the binding mode.

A highly sensitive acidic pH fluorescent probe and its application to HepG2 cells.

A novel acidic fluorescent probe 1 has been designed, synthesized, characterized and evaluated in vivo as optical imaging of intracellular H(+). The design strategy for the probe is based on the change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine dyes. The probe exhibits high sensitivity, good photostability, excellent cell membrane permeability and strong pH dependence.