Multiomics profiling uncovers curdione-induced reproductive toxicity in HTR-8/SVneo cells.

The assessment of medication toxicity and safety is pivotal during pregnancy. Curdione, a sesquiterpene compound extracted from , displays beneficial properties in terms of anti-inflammatory, tumor growth suppression, and anti-coagulative effects. However, its reproductive toxicity and precise mechanism remain unclear.

Senolytics Enhance the Longevity of Caenorhabditis elegans by Altering Betaine Metabolism.

Aging triggers physiological changes in organisms that are tightly linked to metabolic changes. Senolytics targeting many fundamental aging processes are currently being developed. However, the host metabolic response to natural senescence and the molecular mechanism underlying the antiaging benefits of senolytics remain poorly understood.

Integrating Epigenetics, Proteomics, and Metabolomics to Reveal the Involvement of Wnt/β-Catenin Signaling Pathway in Oridonin-Induced Reproductive Toxicity.

Oridonin is the primary active component in the traditional Chinese medicine , displaying anti-inflammatory, anti-tumor, and antibacterial effects. It is widely employed in clinical therapy for acute and chronic pharyngitis, tonsillitis, as well as bronchitis. Nevertheless, the clinical application of oridonin is significantly restricted due to its reproductive toxicity, with the exact mechanism remaining unclear.

The Application of a Physiologically Based Toxicokinetic Model in Health Risk Assessment.

Toxicokinetics plays a crucial role in the health risk assessments of xenobiotics. Classical compartmental models are limited in their ability to determine chemical concentrations in specific organs or tissues, particularly target organs or tissues, and their limited interspecific and exposure route extrapolation hinders satisfactory health risk assessment. In contrast, physiologically based toxicokinetic (PBTK) models quantitatively describe the absorption, distribution, metabolism, and excretion of chemicals across various exposure routes and doses in organisms, establishing correlations with toxic effects.

Cyclodextrin-Modified Amphiphilic Microgel with Bifunctional Domains for Infected Wound Healing via Photothermal Antibacterial Therapy and Nitric Oxide Release.

Bacterial infections are one of the major contributing factors to human mortality, which can cause secondary damage to the injured area, such as leading to inflammation, tissue death, and even personal death. Herein, we developed a novel cyclodextrin (CD)-modified amphiphilic microgel with a 3D network nanostructure that encapsulates hydrophilic indocyanine green (ICG) as a trigger for photothermal therapy (PTT) and hydrophobic ,'-disubstituted-butyl-,'-dinitro-1,4-benzenediamine (BNN6) as a heat-sensitive nitric oxide (NO) donor (CD@I-B) to cope with bacteria-infected wound therapy. This biocompatible microgel showed excellent broad-spectrum antibacterial capability under near-infrared (NIR) laser irradiation, while the photothermal conversion process promotes the deswelling of the microgel and release of NO, which synergistically accelerates wound healing.

Nuciferine Effectively Protects Mice against Acetaminophen-Induced Liver Injury.

Acetaminophen (APAP) overdose still poses a major clinical challenge and is a leading cause of acute liver injury (ALI). N-acetylcysteine (NAC) is the only approved antidote to treat APAP toxicity while NAC therapy can trigger side effects including severe vomiting and even shock. Thus, new insights in developing novel therapeutic drugs may pave the way for better treatment of APAP poisoning.

Integrated Profiles of Transcriptome and mRNA m6A Modification Reveal the Intestinal Cytotoxicity of Aflatoxin B1 on HCT116 Cells.

Aflatoxin B1 (AFB1) is widely prevalent in foods and animal feeds and is one of the most toxic and carcinogenic aflatoxin subtypes. Existing studies have proved that the intestine is targeted by AFB1, and adverse organic effects have been observed. This study aimed to investigate the relationship between AFB1-induced intestinal toxicity and N6-methyladenosine (m6A) RNA methylation, which involves the post-transcriptional regulation of mRNA expression.

Integrated Analysis of Transcriptome and microRNA Profile Reveals the Toxicity of Factors toward Human Colon Adenocarcinoma Cell Line Caco-2.

factors, lathyrane-type diterpenoids isolated from the medical herb L. (), have been associated with intestinal irritation toxicity, but the mechanisms underlying this phenomenon are still unknown. The objective of this study was to evaluate the transcriptome and miRNA profiles of human colon adenocarcinoma Caco-2 cells in response to factors L1 (EFL1) and EFL2.

Effect of Humantenine on mRNA m6A Modification and Expression in Human Colon Cancer Cell Line HCT116.

Humantenine, an alkaloid isolated from the medicinal herb (Gardner & Chapm.) Benth., has been reported to induce intestinal irritation, but the underlying toxicological mechanisms remain unclear.

Oxidation and Antioxidation of Natural Products in the Model Organism .

Natural products are small molecules naturally produced by multiple sources such as plants, animals, fungi, bacteria and archaea. They exert both beneficial and detrimental effects by modulating biological targets and pathways involved in oxidative stress and antioxidant response. Natural products' oxidative or antioxidative properties are usually investigated in preclinical experimental models, including virtual computing simulations, cell and tissue cultures, rodent and nonhuman primate animal models, and human studies.

A fixed nitrous oxide/oxygen mixture as an analgesic for patients with postherpetic neuralgia: study protocol for a randomized controlled trial.

Background: The pain management of postherpetic neuralgia (PHN) remains a major challenge, with no immediate relief. Nitrous oxide/oxygen mixture has the advantages of quick analgesic effect and well-tolerated. The purpose of this study is to investigate the analgesic effect and safety of nitrous oxide/oxygen mixture in patients with PHN.

Pre-mixed nitrous oxide/oxygen mixture treatment of pain induced by postoperative dressing change for perianal abscess: Study protocol for a randomized, controlled trial.

Aim: This study aims to evaluate the safety and analgesic efficacy of pre-mixed nitrous oxide/oxygen mixture treatment of pain induced by dressing change for perianal abscess.

Design: This protocol is a randomized, double-blind, placebo-controlled trial.

Methods: This study will be implemented in the Hospital of Traditional Chinese Medicine.

Two new compounds from supercritical fluid extract of L.

Two new compounds, namely integrin A () and integrin B (), were isolated from the supercritical fluid extract (SFE) of L. Their structures were elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.

Structure elucidation and NMR assignments of a new sesquiterpene of volatile oil from Willd.

A new sesquiterpene, artefrigin (), together with four known sesquiterpenes were isolated from the volatile oil of Willd. The structure of was elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.

Structure elucidation and antimicrobial activities of five compounds from Artemisia integrifolia L.

A new compound, integracid (1), together with four known compounds were isolated from the dichloromethane (CH2Cl2) extract from Artemisia integrifolia L. The structures of compounds (1-5) were elucidated by spectroscopic methods, including ultraviolet, infrared (IR), high resolution-electrospray ionization-mass spectrometry (HR-ESI-MS) and extensive one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) techniques, and by comparison with data reported in the references. Antibacterial activities of the compounds were evaluated against various bacteria.

Synthesis and antitumor activity of sacroflavonoside.

Sacroflavonoside, a new derivative of diphenylethene, was isolated from , which have been found to possess the inhibitory effect on the proliferation of gastric carcinoma cells (MKN-45) in our previous studies. With anisaldehyde (SM-A) as starting material, the sacroflavonoside was synthesized by nucleophilic addition, electrophilic substitution and dehydration cyclization. The structure of sacroflavonoside was established by 1 D (H NMR and C NMR) and 2 D-NMR (HSQC and HMBC) spectral analysis.

Analgesia effect of premixed nitrous oxide/oxygen during the rehabilitation after total knee arthroplasty: a study protocol for a randomized controlled trial.

Background: The morbidity of knee arthritis is increasing among aged people and total knee arthroplasty has been its mainstream treatment to date. Postoperative rehabilitation is an important part of the procedure. However, the intense pain during the functional exercise involved has always been a challenge for both patients and health care professionals.

Structure elucidation and NMR assignments of a new alkaloid from (L.) Soják.

A new alkaloid, named as panalasin (), was isolated from the ethyl acetate extract from (L.) Soják. The structure of was elucidated by spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.

Two new compounds from Krasch.

Two new compounds, named ordosacid A () and ordosacid B (), along with four known compounds: 3,4-dihydroxybenzaldehyde (), -hydroxybenzoic acid (), -hydroxycinnamic acid () and -hydroxycinnamic acid (), were isolated from the ethyl acetate extract of Krasch. The structures of new compounds were elucidated on the basis of spectroscopic methods including UV, IR, ESI-MS, 1D NMR, 2D NMR, HR-ESI-MS and modified Mosher's method.

Antihyperlipidemic Effect, Identification and Isolation of the Lipophilic Components from Artemisia integrifolia.

L (Compositae) is a medicinal and edible plant. To investigate its antihyperlipidemic effect, a crude lipophilic extract and the composing compounds were isolated and fractioned from the petroleum ether extract of aerial parts of using column chromatography on silica gel. The anti-hyperlipidemia effect was studied in a rat model of acute hyperlipidemia, which was induced by triton WR-1339.

Isolation and structure elucidation of two new compounds from artemisia .

Two new compounds, namely arteordoyn A () and arteordoyn B (), together with four known compounds, were isolated from the petroleum ether extract of Krasch. The structures elucidation of and were carried out by 1D-NMR (H and C NMR), 2D-NMR (COSY, HSQC, HMBC and NOESY) and HR-ESI-MS spectral analysis.

Structure elucidation and NMR assignments of a new dihydrochalcone from Empetrum nigrum subsp. asiaticum (Nakai ex H.Ito) Kuvaev.

A new dihydrochalcone, namely 2',5'-dimethyl-3'-methoxy-4',6'-dihydroxyl-dihydrochalcone () together with five known compounds were isolated from the CHCl extract from L. var. K.