Guest adaptative supramolecular sensing strategy for warning the risky aflatoxins in contaminated cereals.

In the face of diversified analytes, it is a great challenge and infeasible task to design and synthesize corresponding macrocyclic hosts to realize the ideal supramolecular sensing. Herein, we proposed a novel supramolecular sensing strategy, guest adaptative assay (GAA), in which analyte was quantitatively transformed under mild conditions to perfectly adapt to macrocyclic host. As a health-threatening "landmine" in cereals, aflatoxins were converted by the aid of alkali hydrolysis to satisfactorily obtain aflatoxins transformants in ionic state, resulting in sensitive response by the guanidinocalix[5]arene•fluorescein reporter pair.

Coordination-induced self-assembly based carbon dot dendrimers as efficient signal labels for electrochemiluminescent immunosensor construction.

In this work, an "on-off" electrochemiluminescent immunosensor for bone alkaline phosphatase (BALP) detection was constructed based on the carbon dot dendrimers (CD DRs) as signal labels and Pt nanoparticles functionalized Ni-phenolic coordination spheres (Pt@Ni-PCS) as quenching labels. The prepared CD DRs with low cytotoxicity were synthesized via coordination-induced self-assembly of carboxyl groups-rich N-doped carbon dots and zirconium oxygen clusters, of which the ECL efficiency was 2-fold enhancement than that of discrete N-doped carbon dots. Benefiting from the efficient quenching effect of Pt@Ni-PCS toward Zr-CD DRs/triethylamine-based ECL system, the prepared immunoassay for BALP detection exhibited a broad linear range with 1 pg/mL to 50 ng/mL and a low limit of detection of 24.

Self-Stirring Microcatalysts: Large-Scale, High-Throughput, and Controllable Preparation and Application.

Herein, we introduce a strategy to develop a kind of unprecedented microcatalyst, which owns self-stirring and catalytic performance based on pneumatic printing and magnetic field induction technology. A spindle-shaped microcatalyst based on metal-organic frameworks (MOFs) with a certain aspect ratio and size can be obtained by tuning the printing parameters and the intensity of the magnetic field. One nozzle can print 18 000 microcatalysts per hour, which provides a prerequisite for the realization of large-scale production in the industrial field.

Hierarchical self-uncloaking CRISPR-Cas13a-customized RNA nanococoons for spatial-controlled genome editing and precise cancer therapy.

CRISPR-Cas13a holds enormous potential for developing precise RNA editing. However, spatial manipulation of CRISPR-Cas13a activity remains a daunting challenge for elaborately regulating localized RNase function. Here, we designed hierarchical self-uncloaking CRISPR-Cas13a-customized RNA nanococoons (RNCOs-D), featuring tumor-specific recognition and spatial-controlled activation of Cas13a, for precise cancer synergistic therapy.

CeO/MXene heterojunction-based ultrasensitive electrochemiluminescence biosensing for BCR-ABL fusion gene detection combined with dual-toehold strand displacement reaction for signal amplification.

An "on-off" nonenzymatic and ultrasensitive electrochemiluminescence (ECL) biosensing platform has been constructed to detect BCR-ABL fusion gene based on CeO/MXene heterojunction and configuration-entropy driven dual-toehold strand displacement reaction (DT-SDR) for signal amplification. The CeO/MXene heterojunction were prepared via one-step hydrothermal method through in situ synthesis of CeO nanocubes on the surface of TiC-MXene nanosheets. Surprisingly, the prepared CeO/MXene heterojunction with good dispersion and excellent conductivity not only significantly enhanced ECL emission of SO/O system, but also acted as good electrode modification materials to provide massive active sites for three-stranded ST/AS/BK complex immobilization.

Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon.

Human epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin.

Dispersion-to-localization of catalytic hairpin assembly for sensitive sensing and imaging microRNAs in living cells from whole blood.

Localized DNA circuits have shown good performance regarding reaction rate and sensitivity for sensing intracellular microRNAs (miRNAs). However, these methods reported recently require large kinds of DNA strands and suffer from low signal-to-background (S/B) ratio, which hinder their clinical application. To circumvent these issues, we herein developed a novel strategy for sensitive sensing and imaging miRNAs in living cells based on dispersion-to-localization of catalytic hairpin assembly (DL-CHA).

Efficient DNA Walker Guided with Well-Regulated Interfacial Tracks for Ultrasensitive Electrochemiluminescence Biosensing.

Herein, highly efficient deoxyribonucleic acid (DNA) walking on electrode surfaces was realized by regulating DNA tracks, which was applied to construct an ultrasensitive electrochemiluminescent (ECL) biosensor for BCR/ABL fusion gene detection. The well-regulated DNA tracks were constructed supersandwich hybridization chain reaction of two DNA strands (L and L) to generate periodic linear dsDNA concatemers, where an exposed L domain closed with blocking strands (BS). The prepared DNA tracks were further assembled onto the surface of the Au nanoparticle-functionalized g-CN nanohybrid (Au@g-CN NHs)-modified electrode, achieving well-regulated interfacial tracks for the DNA walker.