Application of random quality control based on time -series model in ELISA detection of blood donors in Nanjing.

This study introduces a completely Random Quality Control (R-QC) method for Enzyme-Linked Immunosorbent Assay (ELISA) tests and compares it with traditional Fixed Quality Control (F-QC) methods across different testing items. A random quality control system was constructed using a Time-series model, enabling fully random positioning of quality control in ELISA tests. The Coefficient of Variation (CV) for R-QC were found to be lower as compared to F-QC in Hepatitis B surface antigen (HBsAg), anti-Human Immunodeficiency Virus (anti-HIV), and Treponema Pallidum (TP) items, and there were significant differences among them.

Analysis of risk factors for postoperative bleeding and recurrence after laparoscopic myomectomy in patients with uterine fibroids: a retrospective cohort study.

Background: In Asia, the incidence of uterine fibroids (UFs) in women is as high as 1.278%. However, there are few analyses of the prevalence and independent risk factors for bleeding and recurrence after laparoscopic myomectomy (LM).

Association of polymorphisms in the HBG1-HBD intergenic region with HbF levels.

Background: Increased levels of fetal hemoglobin (HbF) can improve the clinical course of the patients with sickle cell anemia (SCA) or β-thalassemia. The HBG1-HBD intergenic region plays an important role in this process. However, very few studies investigated whether the variations in this region have an effect on HbF expression.

Volatile organic compounds emissions from traditional and clean domestic heating appliances in Guanzhong Plain, China: Emission factors, source profiles, and effects on regional air quality.

Solid (biomass and coal) fuels burned for residential heating are major sources of atmospheric volatile organic compounds (VOCs). In this study, VOC samples were collected in-situ from chimneys in 10 typical heating scenarios in rural areas of the Guanzhong Plain. A modified SUMA canister approach was then employed, followed by gas chromatography/mass spectrometry analysis.

Reference intervals for HbA and HbF and cut-off value of HbA for β-thalassemia carrier screening in a Guizhou population of reproductive age.

Objective: The aims of this study were to establish the reference intervals for HbA2 and HbF in a Guizhou population of reproductive age, and to determine the cut-off value of HbA for β-thalassemia carrier screening.

Methods: Hemoglobin analysis was performed on 832 individuals without hypochromic microcytic anemia to calculate the reference intervals for HbA and HbF. Three hundred and ninety one β-thalassemia carriers and non β-thalassemia individuals were analyzed for their HbA levels followed by detecting β-globin gene mutations, then cut-off value of HbA for β-thalassemia carrier screening was determined using ROC curve analysis.

[Effect of Blocking PYR Complex Binding to DNA Site by Peptide Nucleic Acid on γ-Globin Gene Expression].

Objective: To investigate the effect of blocking polypyrimidine complex binding to DNA site by using peptide nucleic acid (PNA) on γ-globin gene expression.

Methods: PYR-PNA, β-PNA and RS-PNA (random sequence-PNA) were designed and synthesized, then were transfected into K562 cells with the cationic liposome lipofectamine 2000 used as vector. The expression of γ-globin gene at both the transcriptional and translational level was detected by RT-PCR and the Western blot respectively at 24 h, 48 h and 72 h after transfection with PNAs.

[Results of thalassemia screening and genetic diagnosis for 13 738 pregnant women].

Objective: To report on the result of thalassemia screening and genetic diagnosis for pregnant women from Guiyang region.

Methods: Prenatal screening for thalassemia was carried out based on erythrocyte parameters and hemoglobin electrophoresis. Single-tube multiplex GAP-PCR and PCR-reverse dot blot hybridization were performed on suspected cases to identify common alpha- and beta- thalassemia mutations, and direct sequencing was used for identifying rare mutations.

[Clonal plasticity of Iris japonica under different soil and canopy conditions in subtropocal evergreen broad-leaved forest].

An investigation was made on the growth indices of Iris japonica growing on the yellow soil and Karst soil within a subtropical evergreen broad-leaved forest and in the forest gaps, aimed to approach the clonal plasticity of I. japonica in heterogeneous habitats. The results showed that the ramet height and diameter and the rhizome diameter of I.