Evaluating resistance of potato cultivars and breeding clones to golden cyst nematode in China.

The golden cyst nematode, Globodera rostochiensis, is a regulatory pathogen that can significantly reduce potato yields and hinder the international trade of potatoes. Unfortunately, this nematode has been reported in southwestern China. Nevertheless, limited information exists on the resistance levels of Chinese potato germplasm, especially primary commercial cultivars, to G.

Exploring plant growth promoting traits and biocontrol potential of new isolated BS-2301 strain in suppressing through various mechanisms.

(Lib.) de Bary is the causative agent of stem white mold disease which severely reduces major crop productivity including soybean and rapeseed worldwide. The current study aimed to explore plant growth-promoting traits and biocontrol of new isolated BS-2301 to suppress through various mechanisms.

Biocontrol of plant parasitic nematodes by bacteria and fungi: a multi-omics approach for the exploration of novel nematicides in sustainable agriculture.

Plant parasitic nematodes (PPNs) pose a significant threat to global crop productivity, causing an estimated annual loss of US $157 billion in the agriculture industry. While synthetic chemical nematicides can effectively control PPNs, their overuse has detrimental effects on human health and the environment. Biocontrol agents (BCAs), such as bacteria and fungi in the rhizosphere, are safe and promising alternatives for PPNs control.

Chromosome-level genome assembly of the cereal cyst nematode Heterodera flipjevi.

As an economically important plant parasitic nematode (PPN), Heterodera filipjevi causes great damage on wheat, and now it was widely recorded in many countries. While multiple genomes of PPNs have been published, high-quality genome assembly and annotation on H. filipjevi have yet to be performed.

No Pairwise Interactions of GmSNAP18, GmSHMT08 and AtPR1 with Suppressed Expression Enhance the Susceptibility of Arabidopsis to Beet Cyst Nematode.

and are two major genes conferring soybean cyst nematode (SCN) resistance in soybean. Overexpression of either of these two soybean genes would enhance the susceptibility of Arabidopsis to beet cyst nematode (BCN), while overexpression of either of their corresponding orthologs in Arabidopsis, and , would suppress it. However, the mechanism by which these two pairs of orthologous genes boost or inhibit BCN susceptibility of Arabidopsis still remains elusive.

Bacterial and Fungal Biocontrol Agents for Plant Disease Protection: Journey from Lab to Field, Current Status, Challenges, and Global Perspectives.

Plants are constantly exposed to various phytopathogens such as fungi, Oomycetes, nematodes, bacteria, and viruses. These pathogens can significantly reduce the productivity of important crops worldwide, with annual crop yield losses ranging from 20% to 40% caused by various pathogenic diseases. While the use of chemical pesticides has been effective at controlling multiple diseases in major crops, excessive use of synthetic chemicals has detrimental effects on the environment and human health, which discourages pesticide application in the agriculture sector.

Self-Assembled Nanonematicide Induces Adverse Effects on Oxidative Stress, Succinate Dehydrogenase Activity, and ATP Generation.

Long-term overuse of chemical nematicides has resulted in low control efficacy toward destructive root-knot nematodes, and continuous development in nanotechnology is supposed to enhance the utilization efficiency of nematicides to meet practical needs. Herein, a cationic star polymer (SPc) was constructed to load fluopyram (flu) and prepare a flu nanoagent. Hydrogen bonding and van der Waals forces facilitated the self-assembly of the flu nanoagent, leading to the breakdown of self-aggregated flu and reducing its particle size to 60 nm.

Current advances in the identification of plant nematode diseases: From lab assays to in-field diagnostics.

Plant parasitic nematodes (PPNs) cause an important class of diseases that occur in almost all types of crops, seriously affecting yield and quality and causing great economic losses. Accurate and rapid diagnosis of nematodes is the basis for their control. PPNs often have interspecific overlays and large intraspecific variations in morphology, therefore identification is difficult based on morphological characters alone.

Population Structure in China Suggests a South-to-North Expansion.

The distribution range of root-knot nematode is rapidly expanding, posing a severe threat to rice production. In this study, the sequences of cytochrome oxidase subunit I () genes of rice populations from all reported provinces in China were amplified and sequenced by PCR. The distribution pattern and phylogenetic tree showed that all 54 populations in China have distinct geographical distribution characteristics; specifically, cluster 1 (southern China), cluster 2 (central south and southwest China), and cluster 3 (central and eastern China).

Silicon Mediated Plant Immunity against Nematodes: Summarizing the Underline Defence Mechanisms in Plant Nematodes Interaction.

Silicon (Si) is known to stimulate plant resistance against different phytopathogens, i.e., bacteria, fungi, and nematodes.

Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a Technology for Rapid and Highly Sensitive Detection of and .

The cereal cyst nematodes and are recognized as cyst nematodes that infect cereal crops and cause severe economic losses worldwide. Rapid, visual detection of cyst nematodes is essential for more effective control of this pest. In this study, recombinase polymerase amplification (RPA) combined with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a (formerly known as cpf1) was developed for the rapid detection of and from infested field samples.

Association of (Nematoda) with Stunted and Chlorotic Potato Plants in Yunnan and Sichuan Provinces in China.

On a global basis, potato cyst nematodes ( spp. Skarbilovich 1959 [Behrens 1975]) are one of the most serious soilborne pathogens in potato ( L.) production.

Origin and Phylogeography of Chinese Cereal Cyst Nematode Revealed by Mitochondrial COI Sequences.

, a globally distributed plant-parasitic nematode, is one of the most significant pests on cereal crops. In China, it is widely distributed in cereal-growing areas of 16 provinces and causes serious yield losses. In the present study, a total of 98 populations of were collected from major wheat-growing regions in China and six other countries.

Opposite Beet Cyst Nematode Infection Phenotypes of Transgenic Arabidopsis Between Overexpressing and and Between Overexpressing and .

The (an ) and are two major cloned genes conferring soybean cyst nematode resistance in Peking-type soybeans, but the application of and in cyst nematode management remains elusive. In this study, and , together with their orthologs in Arabidopsis, (an ) and , were individually transformed into Arabidopsis Col-0 to generate the transgenic lines, and the growth of transgenic plants, beet cyst nematode (BCN) infection phenotypes, and , , and expression patterns were analyzed using Arabidopsis-BCN compatible interaction system, in addition with protein-protein interaction assay. Pulldown and BiFC assays revealed that GmSNAP18 and GmSHMT08 interacted with AtSHMT4 and AtSNAP2, respectively.

Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice.

Three types of nematode-feeding sites (NFSs) caused by M. graminicola on rice were suggested, and the NFS polarized expansion stops before the full NFS maturation that occurs at adult female stage. Root-knot nematodes, Meloidogyne spp.

Development of a Species-Specific SCAR-PCR Assay for Direct Detection of Sugar Beet Cyst Nematode () from Infected Roots and Soil Samples.

Sugar beet cyst nematode (SBCN, ) is an important nematode that causes significant yield losses of 25-50% or more in most areas of sugar beet production worldwide. Rapid and accurate identification of this species is essential to support decisions on pest management. However, the difference between and other spp.

Prevalence and Molecular Diversity of Plant-Parasitic Nematodes of Yam ( spp.) in China, with Focus on spp.

There is little information about nematode pests associated with yam in China. Between 2020 and 2021, surveys of yam fields were conducted to investigate the abundance and prevalence of plant-parasitic nematodes in major yam growing areas. A total of 110 bulk soil samples from the yam rhizosphere and 48 yam tubers were collected from seven counties in Jiangxi and Shandong provinces.

Rapid and Visual Detection of Using Recombinase Polymerase Amplification Combined with Cas12a-Mediated Technology.

is a well-known cyst nematode that causes serious economic losses in sugar beet production every year. Rapid and visual detection of is essential for more effective prevention and control. In this study, a species-specific recombinase polymerase amplification (RPA) primer was designed from a specific sequence-characterized amplified region (SCAR) marker.

Correction: Dihydroxyacetone of wheat root exudates serves as an attractant for Heterodera avenae.

[This corrects the article DOI: 10.1371/journal.pone.

First Report of Meloidogyne graminicola on Rice in Henan Province, China.

Rice (Oryza sativa) is an important food crop in China and root-knot nematode Meloidogyne graminicola has been one of the most important diseases on rice in recently five years (Ju et al. 2020). In August 2020, rice plants were found to be maldeveloped, yellow leaves and hooked root tips in an irrigated paddy field of Yuanyang County, Xinxiang City, Henan Province.

HaCRT1 of Is Required for the Pathogenicity of the Cereal Cyst Nematode.

Cereal cyst nematodes are sedentary biotrophic endoparasites that secrete effector proteins into plant tissues to transit normal cells into specialized feeding sites and suppress plant defenses. To understand the function of nematode effectors in , here, we identified a calreticulin protein HaCRT1, which could suppress the cell death induced by Bax when expressed in . HaCRT1 is synthetized in the subventral gland cells of pre-parasitic second-stage nematodes.

Proteome-Wide Analyses Provide New Insights into the Compatible Interaction of Rice with the Root-Knot Nematode .

The root-knot nematode is an important pathogen in rice, causing huge yield losses annually worldwide. Details of the interaction between rice and and the resistance genes in rice still remain unclear. In this study, proteome-wide analyses of the compatible interaction of the rice cultivar "Nipponbare" (NPB) with were performed.

Dihydroxyacetone of wheat root exudates serves as an attractant for Heterodera avenae.

Heterodera avenae, as an obligate endoparasite, causes severe yield loss in wheat (Triticum aestivum). Investigation on the mechanisms how H. avenae perceives wheat roots is limited.