ULBP2 promotes progression of head and neck squamous cell carcinoma by modulating MAPK signaling pathway.

Background: UL16-binding protein 2 (ULBP2) is significantly overexpressed in diverse cancers, it may also serve as a potential prognostic factor. Nonetheless, the mechanism of action and functions associated with ULBP2 in head and neck squamous cell carcinoma(HNSCC) are unexplored. This study aims to clarify the role and mechanism of ULBP2 in HNSCC and determine whether this molecule promotes tumor progression through modulation multiple downstream MAPK signaling.

Mucosal Hub Bacteria as Potential Targets for Improving High-Fat Diet-Related Intestinal Barrier Injury.

Intestinal barrier injury contributes to multiple diseases such as obesity and diabetes, whereas no treatment options are available. Due to close interactions between mucosal microbiome and intestinal barrier, we evaluated the potential of mucosal bacteria in providing targets for high-fat diet (HFD)-related intestinal barrier injury. Whole-genome metagenomics was used to evaluate mucosal microbiome, while intestinal barrier injury was estimated using serum LPS, FITC-dextran intensity, and ZO-1 protein.

Organic dopant cyclization and significantly improved RTP properties.

The internal rotation of triplet-generating molecules is detrimental to room temperature phosphorescence (RTP) radiation, and this rotation is usually mitigated by doping into rigid microenvironments. The chemical locking of internal rotation units in advance should be an effective strategy but is rarely studied in comparison. Herein, a triplet-generating molecule with two rotatable phenyls (DIA) was designed, synthesized, and then cyclized using two types of bonding bridges.

STAVER: a standardized benchmark dataset-based algorithm for effective variation reduction in large-scale DIA-MS data.

Mass spectrometry (MS)-based proteomics has become instrumental in comprehensively investigating complex biological systems. Data-independent acquisition (DIA)-MS, utilizing hybrid spectral library search strategies, allows for the simultaneous quantification of thousands of proteins, showing promise in enhancing protein identification and quantification precision. However, low-quality profiles can considerably undermine quantitative precision, resulting in inaccurate protein quantification.

Serum proteome reveals distinctive molecular features of H7N9- and SARS-CoV-2-infected patients.

The coronavirus disease 2019 (COVID-19) pandemic has reminded us of human infections with the H7N9 virus and has raised questions related to the clinical and molecular pathophysiological diversity between the two diseases. Here, we performed a proteomic approach on sera samples from patients with H7N9-virus or SARS-CoV-2-virus infection and healthy controls. Compared to SARS-CoV-2, H7N9-virus infection caused elevated neutrophil concentrations, T cell exhaustion, and increased cytokine/interleukin secretion.

Toxicological risk assessment of triadimenol for human exposure, broiler health, and food safety.

Triadimenol, a widely used triazole fungicide, leaves residues that pose risks to broiler health, food safety, and human health. Current studies focus on lab animals, leaving limited data regarding its impact on non-target organisms in agricultural ecosystems. Moreover, the doses in current studies often exceed typical agricultural pollution levels of triadimenol.

Membrane-bound IL-7 immobilized by the CD8 transmembrane region improves efficacy of CD19 CAR-T cell therapy.

Enhancing the efficacy of CD19 CAR-T cell therapy can significantly improve patient outcomes by reducing relapse rates in CD19 + B cell malignancies. Exogenous or transgenic cytokines are often used to boost the expansion and durability of CAR-T cells but pose risks of severe toxicities. A promising approach to address these limitations is to immobilize cytokines on the surface of CAR-T cells using transmembrane (TM) anchor domains.

Kidney Function and Cardiovascular Disease: Evidence from Observational Studies and Mendelian Randomization Analyses.

Unlabelled: Observational studies have identified that declined kidney function was associated with a higher risk of cardiovascular disease (CVD). However, the causation of such associations requires further exploration. Here, we compared the observational associations of various kidney function measurements with CVD and its major subtypes and further assessed the potential causality using Mendelian randomization (MR) analyses with individual-level data of 306,246 participants from the UK Biobank and genome-wide association study summary-level data from FinnGen consortium, Coronary ARtery DIsease Genome wide Replication and Meta-analysis plus The Coronary Artery Disease Genetics (CARDIoGRAMplusC4D) consortium, and MEGASTROKE Consortium, respectively.

Copper-Catalyzed Enantioselective Hydrophosphorylation of Unactivated Alkynes.

P-stereogenic phosphorus compounds are essential across various fields, yet their synthesis via enantioselective P-C bond formation remains both challenging and underdeveloped. We report the first copper-catalyzed enantioselective hydrophosphorylation of alkynes, facilitated by a newly designed chiral 1,2-diamine ligand. Unlike previous methods that rely on kinetic resolution with less than 50 % conversion, our approach employs a distinct dynamic kinetic asymmetric transformation mechanism, achieving complete conversion of racemic starting materials.

A novel method to reconstruct the complex facial nerve defect after extended parotidectomy with masseteric nerve and descending hypoglossal nerve.

This study aims to present a novel technique for reconstructing complex facial nerve defects using the masseteric nerve and descending hypoglossal nerve. Here, we report a case involving a patient with locally advanced parotid malignancy who underwent extended parotidectomy with resection of the invaded facial nerve. Following tumor resection, the proximal end of the facial nerve was inaccessible, leading to the formation of multiple distal branch defects.

Functional reconstruction of tissue defects after total parotidectomy using a modified submandibular gland flap.

Objective: Reconstruction of soft tissue defects after total parotidectomy requires a feasible and effective pedicled flap with sufficient volume. In this study, we introduce a modified submandibular gland flap (SMGF) for functional reconstruction of soft tissue defects resulting from total parotidectomy.

Materials And Methods: This study included 12 patients diagnosed with parotid gland carcinoma undergoing total parotidectomy and ipsilateral selective neck dissection.

Construction of an immune-related gene prognostic model for obese endometrial cancer patients based on bioinformatics analysis.

Background: The tumor microenvironment (TME) affected the prognosis of tumors. However, its effect on the outcomes of obese endometrial cancer (EC) patients had not been reported.

Methods: This research performed a retrospective analysis of the transcriptome profiles and medical data of 503 EC patients.

Field evaluation of oil crop rotations for cadmium remediation and safe vegetable oil production across five sites with varying contamination levels.

Oil crops have the potential to remediate cadmium (Cd)-contaminated farmland while producing safe vegetable oil. However, it is currently unknown whether different oil crops can remediate varying levels of Cd contamination in farmland. This study assessed agricultural fields in southern China contaminated with Cd levels ranging from 0.

Effects of the pesticide carbendazim on broiler chicken health and carbendazim residue levels in broiler tissues.

Pesticide residues may enter the human body through the food chain when livestock and poultry consume pesticide-contaminated feed. Therefore, monitoring and limiting pesticide residues in animal feed and animal-origin foods is necessary. Carbendazim is one of the most frequently detected pesticides in food and feed and has various toxic effects on non-target animals.

Gut microbial features and circulating metabolomic signatures of frailty in older adults.

Frailty, a multidimensional indicator of suboptimal aging, reflects cumulative declines across multiple physiological systems. Although age-related changes have been reported in gut microbiota, their role in healthy aging remains unclear. In this study, we calculated frailty index (FI) from 33 health-related items to reflect the overall health status of 1,821 older adults (62-96 years, 55% female) and conducted multi-omics analysis using gut metagenomic sequencing data and plasma metabolomic data.

The morphological discrepancy of neuromuscular junctions between bilateral paraspinal muscles in patients with adolescent idiopathic scoliosis: A quantitative immunofluorescence assay.

Introduction: Prior studies suggested that neuromuscular factors might be involved in the pathogenesis of adolescent idiopathic scoliosis (AIS). The neuromuscular junction (NMJ) is the important pivot where the nervous system interacts with muscle fibers, but it has not been well characterized in the paraspinal muscles of AIS. This study aims to perform the quantitative morphological analysis of NMJs from paraspinal muscles of AIS.

Dietary Bacillus velezensis KNF-209 supplementation improves growth performance, enhances immunity, and promotes gut health in broilers.

This study aimed to investigate the effects of dietary Bacillus velezensis KNF-209 (BV-KNF-209) on the growth performance, immunity, and gut health of broilers. A total of 540 one-day-old male Cobb-500 broilers were randomly divided into 5 groups of 6 replicates with 18 broilers per replicate. Dietary treatments were corn-soybean meal basal diets supplemented with 0, 50, 100, 200, and 400 mg/kg BV-KNF-209 (CON, BV 50, BV 100, BV 200, and BV 400 groups, respectively) for 42 d.

Magnetic resonance imaging features of progressive familial intrahepatic cholestasis type 3.

Purpose: Progressive familial intrahepatic cholestasis type 3 (PFIC-3) is a rare autosomal recessive cholestatic liver disorder. This study aimed to present the clinical and magnetic resonance imaging (MRI) features of three patients with PFIC‑3.

Methods: The study included three patients with cholestasis and pathogenic variants in the ABCB4 gene identified by next-generation sequencing of a targeted-gene panel or by whole-exome sequencing.

Improving electron transportation and operational lifetime of full color organic light emitting diodes through a "weak hydrogen bonding cage" structure.

Efficient electron-transporting materials (ETMs) are critical to achieving excellent performance of organic light-emitting diodes (OLEDs), yet developing such materials remains a major long-term challenge, particularly ETMs with high electron mobilities (s). Herein, we report a short conjugated ETM molecule (PICN) with a dipolar phenanthroimidazole group, which exhibits an electron mobility of up to 1.52 × 10 cm (V s).