Manipulating the nucleolar serine-rich protein Srp40p in Saccharomyces cerevisiae may improve isobutanol production.

Isobutanol represents a promising second-generation biofuel. Saccharomyces cerevisiae can produce minor quantities of isobutanol as a byproduct. Increasing yeast tolerance to isobutanol is a crucial step toward achieving higher production levels.

Improving isobutanol tolerance and titers through EMS mutagenesis in Saccharomyces cerevisiae.

Improving yeast tolerance toward isobutanol is a critical issue enabling high-titer industrial production. Here, we used EMS mutagenesis to screen Saccharomyces cerevisiae with greater tolerance toward isobutanol. By this method, we obtained EMS39 with high-viability in medium containing 16 g/L isobutanol.