Controllable Synthesis and Ultrahigh Proton Conduction of a Hydrogen-Bond Network.

The functionalization of polyoxometalates with organic ligands provides a new-style strategy to accurately incorporate polyoxometalates with advanced functional organic moieties on their surfaces, the development of which has attracted increasing research interest due to the potential applications. A germanium tungstate Na(HO)[{Ru(bpy)}{WO(CO)}(GeWO)]·27HO (bpy = 2,2'-bipyridine) with two ligands covalently modified was triumphantly synthesized, using the conventional one-pot hydrothermal method. It was systematically characterized by thermogravimetric analysis (TGA), elemental analysis, infrared (IR) spectroscopy, single-crystal X-ray diffraction, X-ray photoelectron spectroscopy (XPS), powder diffraction (PXRD), scanning electronic microscopy (SEM), and ultraviolet-visible (UV-vis) spectroscopy.

A Dawson-type {PW} modified using phenylphosphonic acid with excellent proton conductivity.

A case of organic-inorganic hybridized phosphotungstate modified using aromatic organophosphonic acid, KNaH[KCo(HO)PWO{(PhPO)}]·48HO (1), was successfully synthesized in conventional aqueous solution. The prominent structural feature is that the total structure of [KPWO{(PhPO)}] resembles a V-shaped structure, which was stabilized by two [Co(HO)] ions. Furthermore, it can be connected into a three-dimensional mesh structure using K ions.

Quantum-Dot-Encoded Beads-Enhanced CRISPR/Cas-Based Lateral-Flow Assay for the Amplification-Free, Sensitive, and Rapid Detection of Nucleic Acids in Breast Cancer.

Nucleic acid detection plays a pivotal role in the accurate diagnosis of diseases. The CRISPR/Cas detection system, noted for its significant utility in a variety of applications, often necessitates enhanced sensitivity or specific signal amplification strategies, particularly for detecting low-abundance biomarkers. In this study, we present a quantum-dot-encoded beads (QDB)-energized CRISPR/Cas12-based lateral-flow assay (QDB-CRISPR-LFA).

Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape.

Sclerotinia stem rot (SSR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating diseases for several major oil-producing crops. Despite its impact, the genetic basis of SSR resistance in plants remains poorly understood. Here, through a genome-wide association study, we identify a key gene, BnaA07.

A High Nucleus Cu-Incorporated Giant Phosphotungstate with Photocatalytic Oxidation C-H of Toluene.

Exploring a novel photocatalyst for catalytic oxidation of toluene is a sustainable strategy for energy conversion in times of an energy crisis. However, designing an effective photocatalyst for the conversion of toluene remains challenging. Herein, a novel organic monophosphonate-modified high nucleus Cu-incorporated polyoxotungstate, KH[{Cu(HO)}{Cu(OPCHCOO)(1,4,9-α-PWO)}]·Cl·60HO (), has been intentionally synthesized by a self-assembly process utilizing conventional aqueous method.

Biosensors based on potent miniprotein binder for sensitive testing of SARS-CoV‑2 variants of concern.

The miniprotein binder TRI2-2 was employed as an antibody alternative to build a single antibody-coupled TRI2-2 based gold nanoparticle-based lateral flow immunoassay (AT-GLFIA) biosensor. The biosensor provides high specificity and affinity binding between TRI2-2 and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) spike antigen receptor binding domain (S-RBD). It also enables rapid testing of wild-type (WT), B.

Construction of Hexameric Ru-Substitution POMs to Improve Photocatalytic H Evolution.

Converting solar energy into storable hydrogen energy by employing green photocatalytic technology offers a reliable alternative for meeting the energy crisis. The polyoxometalates are a promising candidate for hydrogen production photocatalysts because of their unique electronic and structural properties and controllable design at the molecular level. Introducing noble metals was proven to be an effective method to greatly enhance the photocatalytic efficiency of polyoxometalates.

Hyperhomocysteinemia potentiates megakaryocyte differentiation and thrombopoiesis via GH-PI3K-Akt axis.

Hyperhomocysteinemia (HHcy) is closely associated with thrombotic diseases such as myocardial infarction and stroke. Enhanced platelet activation was observed in animals and humans with HHcy. However, the influence of HHcy on thrombopoiesis remains largely unknown.

Hyperhomocysteinemia lowers serum testosterone concentration via impairing testosterone production in Leydig cells.

Hyperhomocysteinemia (HHcy) plays a salient role in male infertility. However, whether HHcy interferes with testosterone production remains inconclusive. Here, we reported a lower serum testosterone level in HHcy mice.

Whole-genome sequencing of species isolated from the healthy human eye and description of sp. nov.

Currently, the genus comprises 76 recognized species. Members of are mostly isolated from environmental, animal, and plant sources. This report describes and proposes a novel species of isolated from clinical specimens of the human ocular surface.

sp. nov., sp. nov., and sp. nov., isolated from soil of the Qinghai-Tibetan Plateau.

Six novel bacterial strains, designated CY22, CY357, LJ419, LJ53, CY399 and CY107 were isolated from soil samples collected from the Qinghai-Tibetan Plateau, PR China. Cells were aerobic, rod-shaped, yellow-pigmented, catalase- and oxidase-positive, Gram-stain-negative, non-motile and non-spore-forming. All strains were psychrotolerant and could grow at 0 °C.

The species-level microbiota of healthy eyes revealed by the integration of metataxonomics with culturomics and genome analysis.

Objectives: To characterize the healthy ocular surface microbiota at the species level, including cultured and uncultured taxa.

Methods: We integrated the metataxonomic method with culturomics and genome sequencing analysis of selected isolated strains to better illustrate the taxonomic structure of the ocular surface microbiota. The metataxonomics used the full-length 16S rRNA gene sequences and the operational phylogenetic unit strategy, which can precisely identify the cultured and uncultured or potentially new taxa to species level based on the phylogenetic tree constructed.

sp. nov. and sp. nov., two new rapidly growing mycobacterial species.

Four bacterial strains (LJ126/S18 and Z-34/S20) recovered from faecal samples of Tibetan antelopes on the Qinghai-Tibet Plateau of China were analysed using a polyphasic approach. All four isolates were aerobic, short rod-shaped, non-motile, Gram-stain-positive, acid-fast and fast-growing. Phylogenetic analyses based upon 16S rRNA and whole-genome sequences showed that the two pair of strains formed two distinct branches within the evolutionary radiation of the genus .

Decision-making interaction among stakeholders regarding construction and demolition waste recycling under different power structures.

The market power and competition scenario of recycling enterprises and landfills may change in the future due to the environmental pressure caused by landfills and the environmental potential of construction and demolition waste (C&DW) recycling. In this context, how these changes will affect the economic performance of enterprises and the environmental performance of the whole society remains unclear, along with how the willingness to pay and the environmental awareness of contractors will affect the pricing decisions of recycling enterprises and landfills. This study investigates the charging and recycling problem under different power structures in the reverse supply chain of C&DW, which is composed of waste generators (construction contractors) and two disposers (recycling enterprises and landfills).

sp. nov. and sp. nov., isolated from Tibetan antelope () and plateau pika (), respectively.

Four novel strains (592, S592, MF47 and SMF47) were isolated from Tibetan antelopes () and plateau pikas (), respectively. The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria. The 16S rRNA gene sequences of the four strains showed highest similarities to DSM 10552 (98.

EZH2-inhibitor DZNep enhances apoptosis of renal tubular epithelial cells in presence and absence of cisplatin.

Background: The enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase and induces the trimethylation of histone H3 lysine 27 (H3K27me3) in the promoter of many key genes; EZH2 acts as a transcriptional repressor and is an epigenetic regulator for several cancers. However, the role of EZH2 in nonneoplastic diseases, such as kidney diseases, is unknown and has been investigated.

Materials And Method: NRK-52E cells were treated with DZNep, a potent inhibitor of EZH2, with different concentrations and for different times to evaluate the apoptosis level of NRK-52E cells by Western blot and Flow cytometry analysis.

sp. nov., isolated from leaves of on the Qinghai-Tibet Plateau.

Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144 and 4053, were isolated from leaves of on the Qinghai-Tibet Plateau, PR China. Cells were aerobic, catalase-positive and oxidase-negative. Colonies on Reasoner's 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation.

sp. nov. and sp. nov. isolated from plateau pika () on the Qinghai-Tibet plateau of China.

Four novel bacterial strains, designated Z294, Z311, Z443 and Z446, were isolated from the intestinal contents of plateau pika () on the Qinghai-Tibet Plateau of China. Cells were Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile and short-rod shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the four isolates belong to the genus , but clearly separate from the currently recognized species.

sp. nov., isolated from plateau pika ().

Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-A and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau. Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C.

sp. nov., isolated from the intestinal contents of plateau pika () on the Qinghai-Tibet Plateau.

Two Gram-staining-positive, catalase-positive, oxidase-negative, aerobic, non-motile, irregular rod-shaped bacterial strains (Z350 and Z527) were isolated from intestinal contents of plateau pika () from the Qinghai-Tibet Plateau, PR China. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain Z350 belongs to the genus (family ) but clearly differs from the currently recognized species DSM 101040 (98.4 % similarity) and DSM 27763 (97.

sp. nov. isolated from the leaves of Dandelion () on the Qinghai-Tibetan Plateau.

Two strains (S-1072 and 1626) of Gram-stain-negative, oxidase- and catalase-positive, aerobic, rod-shaped, motile bacteria with a single polar flagellum, were isolated from the leaves of Dandelion () on the Qinghai-Tibet Plateau of China. The cells grew optimally at 28 °C, pH 7.0 and with 0.