Strain SYSU D00308, a short-rod-shaped bacterium, was isolated from a sandy soil collected from the Gurbantunggut Desert, Xinjiang, PR China. Strain SYSU D00308 was Gram-stain-negative, aerobic, pink-pigmented, non-motile, catalase- and oxidase-positive. The strain grew at 4-37 ℃, pH 5.
View Article and Find Full Text PDFA novel rod-shaped bacterium, designated as strain SYSU D60015 that formed yellowish colonies was isolated from a sandy soil collected from the Kumtag Desert in Xinjiang, China. Cells were Gram-stain-negative, oxidase-positive, catalase-negative and motile with a single polar flagellum. Growth optimum occurred between 28 and 37 °C, pH 7.
View Article and Find Full Text PDFTwo Gram-stain-negative, aerobic, milk-white coloured, non-motile, short rod-shaped bacteria, designated as strains SYSU D60010 and SYSU D60012, were isolated from sand samples collected from the Taklimakan Desert of Xinjiang Province in China. Both strains were positive for oxidase, catalase and nitrate reduction, but negative for amylase, HS production, hydrolysis of gelatin and cellulase. Strains SYSU D60010 and SYSU D60012 grew well at 28 °C, at pH 7 and had the same NaCl tolerance range of 0-1 % (w/v).
View Article and Find Full Text PDFAntonie Van Leeuwenhoek
July 2024
An aerobic, Gram-stain-negative bacterium, designated as SYSU D00382, was sourced from soil of Gurbantunggut Desert, PR China. The strain was short-rod-shaped, oxidase-positive and catalase-negative, with yellow-colored, convex, round, and smooth colonies on TSA plate. Growth and proliferation occurred at 4-37 °C (optimal: 28-30 °C), pH 5.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
June 2024
Two Gram-stain-positive, aerobic, oxidase- and catalase-negative, non-motile, and short rod-shaped actinomycetes, named SYSU T00b441 and SYSU T00b490, were isolated from tidal flat sediment located in Guangdong province, PR China. The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between SYSU T00b441 and SYSU T00b490 were 99.3, 99.
View Article and Find Full Text PDFBarkol Lake, a shrinking hypersaline lake situated in the northeast of Xinjiang, China, has experienced the exposure of its riverbed and the gradual drying up of its original sediment due to climate change and human activities, resulting in the formation of alkaline soils. These changes have correspondingly altered the physicochemical characteristics of the surrounding environment. Microorganisms play a crucial role, with special functioning involved in various nutrient cycling and energy transfer in saline lake environments.
View Article and Find Full Text PDFTwo novel bacterial strains, designated as SYSU D00823 and SYSU D00873, were isolated from sandy soil of the Gurbantunggut Desert in Xinjiang, north-west China. SYSU D00823 and SYSU D00873 shared 99.0 % 16S rRNA gene sequence identity, and were both most closely related to 12157 with 96.
View Article and Find Full Text PDFIn this work, a novel fluorescence sensor UiO-66-PSM based on post-synthetic modified metal-organic frameworks was prepared for the detection of berberine hydrochloride (BBH) in the traditional Chinese herb . UiO-66-PSM was synthesized by a simple Schiff base reaction with UiO-66-NH and phthalaldehyde (PAD). The luminescence quenching can be attributed to the photo-induced electron transfer process from the ligand of UiO-66-PSM to BBH.
View Article and Find Full Text PDFDryland covers >35 % of the terrestrial surface and the global extent of dryland increases due to the forecasted increase in aridity driven by climate change. Due to the climate change-driven aridity ecosystems, deserts provide one of the most hostile environments for microbial life and survival. Therefore, a detailed study was carried out to explore the deserts with different aridity levels (exposed to severe climate change) influence on microbial (bacteria, fungi, and protist) diversity patterns, assembly processes, and co-occurrence.
View Article and Find Full Text PDFTwo novel bacterial strains, designated as SYSU D00344 and SYSU D00433, were isolated from soil of Gurbantunggut Desert in Xinjiang, north-west PR China. Cells of both strains were Gram-stain-negative, aerobic, short-rod-shaped, catalase-positive and non-motile. Oxidase activities of SYSU D00344 and SYSU D00433 were negative and positive, respectively.
View Article and Find Full Text PDFA pink-pigmented bacterium, designated as strain SYSU D00476, was isolated from sandy soil collected from the Kumtag Desert in China. Colonies were opaque, smooth and of a slight convexity with a clearly defined border. Cells were rod-shaped, Gram-stain-negative, catalase- and oxidase-positive.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
October 2023
A Gram-stain-negative, aerobic, short rod-shaped, yellow bacterium, designated SYSU DXS3180, was isolated from forest soil of Danxia Mountain in PR China. Growth occurred at 15-37 °C (optimum, 28-30 °C), pH 6.0-10.
View Article and Find Full Text PDFNPJ Biofilms Microbiomes
September 2023
Deserts occupy one-third of the Earth's terrestrial surface and represent a potentially significant reservoir of microbial biodiversity, yet the majority of desert microorganisms remain uncharacterized and are seen as "microbial dark matter". Here, we introduce a multi-omics strategy, culturomics-based metagenomics (CBM) that integrates large-scale cultivation, full-length 16S rRNA gene amplicon, and shotgun metagenomic sequencing. The results showed that CBM captured a significant amount of taxonomic and functional diversity missed in direct sequencing by increasing the recovery of amplicon sequence variants (ASVs) and high/medium-quality metagenome-assembled genomes (MAGs).
View Article and Find Full Text PDFMolecularly imprinted polymers have received wide attention from various fields owing to their pre-designable, recognition ability, and practicality. However, the disadvantages of the traditional embedding method, which include a slow recognition rate, uneven site recognition, low binding capacity, and incomplete template molecule elution, limit the development of molecular imprinting technology. Surface molecular imprinting techniques have been developed to effectively solve these problems, and different materials are used as carriers in the synthesis of molecularly imprinted polymers.
View Article and Find Full Text PDFTwo Gram-stain-negative strains, designated as SYSU D00286 and SYSU D00782, were isolated from a sand sample collected from the Kumtag Desert in Xinjiang, north-west China. Cells were aerobic, non-motile and positive for both oxidase and catalase. Growth occurred at 4-37 °C (optimum, 28-30 °C), pH 6.
View Article and Find Full Text PDFA novel orange-coloured bacterium, designated strain SYSU D00508, was isolated from a sandy soil sampled from the Kumtag Desert in China. Strain SYSU D00508 was aerobic, Gram-stain-negative, oxidase-positive, catalase-positive and non-motile. Growth occurred at 4-45°C (optimum 28-30°C), pH 6.
View Article and Find Full Text PDFAn actinobacterium, designated as SYSU T00001, was isolated from a tidal flat sediment sample from Guangdong province, China. Cells were Gram-stain-positive, aerobic, motile and short rod-shaped. Colonies on marine agar 2216 were smooth, yellow-pigmented, and circular with low convexity.
View Article and Find Full Text PDFBackground: The microbiome of the Sinai Desert farming system plays an important role in the adaptive strategy of growing crops in a harsh, poly-extreme, desert environment. However, the diversity and function of microbial communities under this unfavorable moisture and nutritional conditions have not yet been investigated. Based on culturomic and metagenomic methods, we analyzed the microbial diversity and function of a total of fourteen rhizosphere soil samples (collected from twelve plants in four farms of the Sinai desert), which may provide a valuable and meaningful guidance for the design of microbial inoculants.
View Article and Find Full Text PDFTwo strains designated as SYSU D01084 and SYSU D00799, were isolated from a sandy soil sample collected from Gurbantunggut Desert in Xinjiang, north-west China. Cells of both strains were Gram-stain-negative, strictly aerobic, long-rod-shaped, oxidase- and catalase-negative, motile or non-motile. Colonies were circular, translucent, convex, smooth and light-yellow in color on R2A agar.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
February 2022
A novel Gram-stain-negative, aerobic, oxidase-positive, catalase-positive, non-motile, short rod-shaped, red-pigmented strain, designated as SYSU D00434, was isolated from a dry sandy soil sample collected from the Gurbantunggut desert in Xinjiang, north-west PR China. Strain SYSU D00434 was found to grow at 4-37 °C (optimum, 28-30 °C), pH 6.0-8.
View Article and Find Full Text PDFTwo bacterial strains, designated as SYSU D00720 and SYSU D00722, were isolated from a desert sandy soil sample collected from Gurbantunggut Desert in Xinjiang, north-west China. Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped, oxidase-positive and catalase-negative. Colonies were circular, opaque, convex, smooth, orange on Reasoner's 2A (R2A) agar.
View Article and Find Full Text PDFStrain SYSU D01096 was isolated from a sandy soil sample collected from Gurbantunggut Desert in Xinjiang, PR China. Phylogenetic analysis of the nearly full-length 16S rRNA gene sequence revealed that strain SYSU D01096 belonged to the family Acetobacteraceae and was closest to Rubritepida flocculans DSM 14296 (96.0% similarity).
View Article and Find Full Text PDFStreptomycetes have been, for over 70 years, one of the most abundant sources for the discovery of new antibiotics and clinic drugs. However, in recent decades, it has been more and more difficult to obtain new phylotypes of the genus Streptomyces by using conventional samples and culture strategies. In this study, we combined culture-dependent and culture-independent approaches to better explore the Streptomyces communities in desert sandy soils.
View Article and Find Full Text PDFA novel pale orange-coloured bacterium, designated strain SYSU D00532, was isolated from sandy soil collected from the Gurbantunggut desert in Xinjiang, PR China. Cells of strain SYSU D00532 were found to be aerobic, Gram-stain-negative, oxidase-positive, catalase-positive, motile and rod-shaped with a single polar or subpolar flagellum. Growth occurred at 15-45 °C (optimum, 28-37 °C, pH 5.
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