Comput Struct Biotechnol J
December 2024
Effective subunit vaccines for tuberculosis (TB) must target antigenic components at various stages of infection. In this study, we constructed fusion proteins using secreted antigens from (), specifically ESAT6, CFP10, MPT64, and Rv2645 from the proliferation stage, along with latency-associated antigens Rv1738 and Rv1978. The resulting fusion proteins, designated LT33 (ESAT6-CFP10-Rv1738) and LT28 (MPT64-Rv1978-Rv2645), were combined with an adjuvant containing dimethyldioctadecylammonium bromide (DDA), polyriboinosinic polyribocytidylic acid (PolyI:C), and cholesterol to construct subunit vaccines.
View Article and Find Full Text PDFThe design and development of highly active non-noble metal electrocatalysts for the oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) are crucial for metal-air batteries. In this work, the electrocatalytic performance of different -block metal (PM = Sn, Sb, Pb and Bi) atoms embedded in the g-CN monolayer (PM@g-CN, = 1-3) for the OER and ORR was systematically investigated by density functional theory (DFT). The strong interaction between PM atoms and g-CN substrates indicates the good stability of PM@g-CN catalysts.
View Article and Find Full Text PDFBioactive peptides have received significant attention due to their natural origin, low toxicity, and targeting specificity in the past decade. This study identified highly active ACE/XO inhibitors using molecular simulation and online databases and validated their in vitro antioxidant activity and the mechanisms of molecular interactions. According to computer predictions, Asp-Gly-Gly (DGG) and Asp-Gly-Met (DGGM) were identified as potential hydrolysates of common gastrointestinal peptidases with well water-soluble, non-toxic, and non-allergenic.
View Article and Find Full Text PDFSensors (Basel)
September 2024
Aiming at the problems of a large volume, slow processing speed, and difficult deployment in the edge terminal, this paper proposes a lightweight insulator detection algorithm based on an improved SSD. Firstly, the original feature extraction network VGG-16 is replaced by a lightweight Ghost Module network to initially achieve the lightweight model. A Feature Pyramid structure and Feature Pyramid Network (FPN+PAN) are integrated into the Neck part and a Simplified Spatial Pyramid Pooling Fast (SimSPPF) module is introduced to realize the integration of local features and global features.
View Article and Find Full Text PDFThis study developed a new metallography-property relationship neural network (MPR-Net) to predict the relationship between the microstructure and mechanical properties of 316L stainless steel built by laser powder bed fusion (LPBF). The accuracy R of MPR-Net was 0.96 and 0.
View Article and Find Full Text PDFBioengineering (Basel)
September 2024
Control tissue is essential for ensuring the precision of semiquantitative analysis in back-table fluorescence imaging. However, there remains a lack of agreement on the appropriate selection of control tissues. To evaluate the back-table fluorescence imaging performance of different normal tissues and identify the optimal normal tissue, a cohort of 39 patients with orbital tumors were enrolled in the study.
View Article and Find Full Text PDFBackground And Aims: Various inflammatory and immune cytokines play key roles in the progression of hepatitis B virus (HBV)-related liver cirrhosis (LC). This study explored the relationship between single nucleotide polymorphisms (SNPs) in cytokines with the combined effect of polymorphisms and gender-polymorphisms interaction and LC risk.
Methods: In this study, a case-control design was used, samples were selected from 45 patients with hepatitis B-related cirrhosis and 45 age-gender-matched chronic HBV-infected patients without cirrhosis attending the tumor hospital of Wuwei Academy of Medical Sciences.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
June 2024
Objectives: Retinal vein occlusion (RVO) is the second most common retinal vascular disease worldwide, and the retinal perfusion status is closely related to the prognosis of the disease. Macular perfusion status is particularly correlated with visual acuity. This study aims to investigate the changes in macular perfusion indicators in RVO using optical coherence tomography angiography (OCTA) and analyze the correlation between macular perfusion status and visual acuity.
View Article and Find Full Text PDFCholangiocarcinoma (CCA) is a bile duct malignancy with a dismal prognosis. This study systematically investigated the role of the ribosomal protein S6 () gene, which is dependent in CCA. We found that RPS6 upregulation in CCA tissues was correlated with a poor prognosis.
View Article and Find Full Text PDFLithium metal batteries paired with high-voltage LiNiMnO (LNMO) cathodes are a promising energy storage source for achieving enhanced high energy density. Forming durable and robust solid-electrolyte interphase (SEI) and cathode-electrolyte interface (CEI) and the ability to withstand oxidation at high potentials are essential for long-lasting performance. Herein, advanced electrolytes are designed via trio-functional additives to carbonate-based electrolytes for 5 V Li||LNMO and graphite||LNMO cells achieving 88.
View Article and Find Full Text PDFObjective: This study aimed to explore the relationship between serum lipoprotein(a) (LP(a)) levels and early neurological deterioration (END) in patients with acute ischemic stroke (AIS) after thrombolysis.
Methods: In total, 236 patients with AIS after thrombolysis were enrolled in this study. Serum LP(a) levels were measured on admission after thrombolysis.
There is growing interest in the origin, preparation, and application of bioactive peptides. This study investigated the effect of 6 enzymes on the structural, physicochemical properties, antioxidant activities, and antidiabetic potential of defatted fresh goat milk. Structural and functional changes resulting from enzymatic hydrolysis were assessed using gel electrophoresis, laser particle size analysis, multi-spectroscopy, and evaluations of foaming and emulsification properties.
View Article and Find Full Text PDFIn this work, we report the synthesis of a new thiosemicarbazone-based drug of N'-(di(pyridin-2-yl)methylene)-4-(thiazol-2-yl)piperazine-1-carbothiohydrazide (H) featuring a thiazole spectator for efficient coordination with Cu(II) to give [CuCl()] () and [Cu(NO)()] (). Both and exhibit dimeric structures ascribed to the presence of di-2-pyridylketone moieties that demonstrate dual functions of chelation and intermolecular bridging. H, , and are highly toxic against hepatocellular carcinoma cell lines Hep-G2, PLC/PRF/5, and HuH-7 with half maximal inhibitory concentration (IC) values as low as 3.
View Article and Find Full Text PDFJ Stroke Cerebrovasc Dis
November 2024
Objective: The purpose of this study is to the relationship between peripheral apolipoproteins and the prognosis of patients with acute ischemic stroke (AIS) after thrombolysis.
Methods: A total of 231 AIS patients with thrombolysis was enrolled. Serum apolipoproteins were measured on admission after thrombolysis.
To understand the role of an additional coordination site in the linker in chirality sensing, we designed and synthesized an S-2-methylbutanamido-substituted -phthalic diamide-linked zinc bisporphyrinate, [Zn(S-MAABis)] and investigated its ability to sense the chirality of amino acid esters. The H NMR spectra and the crystal structure showed that the amido oxygen adjacent to the chiral carbon was coordinated with zinc. NMR and UV-vis titration showed that the binding of [Zn(S-MAABis)] to amino acid esters occurred via two equilibria, forming 1:1 and 1:2 host-guest complexes.
View Article and Find Full Text PDFAims: Diabetic retinopathy (DR) results from complex genetic and metabolic interactions. Unraveling the links between blood metabolites and DR can advance risk prediction and therapy.
Methods: Leveraging Mendelian Randomization (MR) and Linkage Disequilibrium Score Regression (LDSC), we analyzed 10,413 DR cases and 308,633 controls.