Publications by authors named "Wenhao Pang"
Article Synopsis
- Polyarginine is a cell-penetrating peptide that enhances drug delivery through its ability to cross cell membranes, and the study focuses on improving siRNA delivery systems.
- The newly synthesized PCL-R15 diblock polymer showed stronger interactions with cells and improved transfection efficiency compared to its predecessor, R15, by forming condensed nanoplexes.
- The research identified that endocytosis routes, like caveolae and clathrin-mediated pathways, play key roles in cellular uptake, but the entrapment of nanoplexes in lysosomes limits effective siRNA delivery.
A safe and efficient delivery system is critical for clinical application of siRNA. However, the conventional electrostatic interaction-based siRNA nanoplexes with bulk mixing preparation were always unsatisfactory for its stability and safety. In this study, the new core-shell lipid/PCL-PEI/siRNA nanoparticles (LPS NPs) endowing holonomic constraint of siRNA in the inner core were prepared by microfluidic technology.
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- The study examined how phenanthrene (PHE) attaches to different soil organic matter fractions, focusing on humic acid (HA) and humin (HM) from organic and mineral soils.
- Results showed that while the overall polarity of soil organic materials didn't directly correlate with PHE's sorption, surface polarity did positively influence it.
- Additionally, HMs had a higher PHE sorption capacity compared to HAs due to their greater aliphatic carbon content, and the association of some aliphatic structures with minerals in mineral soils limited their interaction with PHE.
Systemic delivery of siRNA is the most challenging step to transfer RNAi to clinical application for breast cancer therapy. In this study, the tumor targeted, T7 peptide modified core-shell nanoparticles (named as T7-LPC/siRNA NPs) were constructed to achieve effective systemic delivery of siRNA. The core-shell structure of T7-LPC/siRNA NPs enables them to encapsulate siRNA in the core and protect it from RNase degradation during circulation.
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- A new cationic lipid (ssGLCL) was developed for delivering siRNAs into cells, featuring hydrophilic lysine heads, hydrophobic tails, and a unique disulfide bond bridge.
- The study compared ssGLCL with a control lipid (ccGLCL) that had a stable bond, finding that both formed nanoparticles capable of effective siRNA delivery.
- Despite lower uptake of siRNA in breast cancer cells, ssGLCL showed enhanced down-regulation of targeted mRNA and protein levels, likely due to better release of siRNA from lysosomes after disulfide bond cleavage.
A somatostatin analog, vapreotide (VAP), can be used as a ligand for targeting drug delivery based on its high affinity to somatostatin receptors (SSTRs), which is overexpressed in many tumor cells. RNA interference plays an important role on downregulation of vascular endothelial growth factor (VEGF), which is important for tumor growth, progression and metastasis. To improve tumor therapy efficacy, the vapreotide-modified core-shell type nanoparticles co-encapsulating VEGF targeted siRNA (siVEGF) and paclitaxel (PTX), termed as VAP-PLPC/siRNA NPs, were developed in this study.
View Article and Find Full Text PDFOur previous study had reported that cholesterol-grafted poly(amidoamine) (rPAA-Chol polymer) was able to self-assemble into cationic nanoparticles and act as a potential carrier for siRNA transfection. In this study, the core-shell type lipid/rPAA-Chol hybrid nanoparticles (PEG-LP/siRNA NPs and T7-LP/siRNA NPs) were developed for improving in vivo siRNA delivery by modifying the surface of rPAA-Chol/siRNA nanoplex core with a lipid shell, followed by post-insertion of polyethylene glycol phospholipid (DSPE-PEG) and/or peptide (HAIYPRH, named as T7) modified DSPE-PEG-T7. The integrative hybrid nanostructures of LP/siRNA NPs were evidenced by dynamic light scattering (DLS), confocal laser scanning microscope (CLSM), cryo-transmission electron microscope (Cryo-TEM) and surface plasmon resonance (SPR) assay.
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