Olaparib-associated toxicity in cancer patients: a systematic review and meta-analysis.

Purpose: To investigate the characteristics of olaparib-associated adverse events (AEs) in cancer patients.

Methods: Databases were searched for phase II and III randomized controlled trials (RCTs) involving olaparib treatment up to March 2024. A systematic assessment was performed.

Neurological toxicities with poly (ADP-ribose) polymerase inhibitors in cancer patients: a systematic review and meta-analysis.

We conducted this meta-analysis to investigate neurological toxicities with poly (ADP-ribose) polymerase inhibitors (PARPis) in cancer patients. Databases were searched for randomized controlled trials (RCTs) from 1 January 2000 to 1 November 2023. Forty-six RCTs and 9529 patients were included.

The Molecular Mechanism of FABP4 Inhibition Effects of GAS and 4-HBA in Blume Was Discussed Based on NMR and Molecular Docking.

To isolate gastrodin (GAS), 4-hydroxybenzyl alcohol (4-HBA), and phenolic compounds from Chinese medicine Blume, and to explore the binding mode of fatty acid binding protein 4 (FABP4/aP2) that is closely related to macrophage inflammation, we study their anti-inflammatory targets. After the ultrasonic extraction of the main active components with 70% ethanol, three resins and three eluents were selected, and eight phenolic monomers with similar structures, such as gastrodin and 4-hydroxybenzyl alcohol, were isolated from by AB-8 macroporous resin and silica gel column chromatography and eluted with the CHCl-MeOH gradient. Their structures were identified by HPLC and nuclear magnetic resonance (NMR).

Swieteliacates S-U, phragmalin limonoids, from the leaves of .

Three novel phragmalin-type limonoids, swieteliacates S-U (1-3), were isolated from leaves, alongside four previously identified limonoids (4-7). The structures, encompassing absolute configurations, were delineated through 1D and 2D NMR analyses, high-resolution mass spectrometry (HR-MS), and NMR and ECD calculations. Swieteliacate S (1) is a distinctive cryptate comprising a tricyclo[4.

Effect of et and on liver protection mechanism based on pharmacokinetics and metabonomics.

Objective: Based on metabonomics technology of high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) and hydrogen nuclear magnetic resonance spectroscopy (H NMR), the pharmacokinetic characteristics and therapeutic mechanism of et (RhRR, Dahuang in Chinese), (EuS, Tubiechong in Chinese) combined with RhRR acting on acute liver injury were explored.

Methods: Models of acute liver injury were established, and the pharmacokinetic methods of five components of RhRR-EuS in rats were found by HPLC-MS/MS. The liver tissues of different groups of mice were analyzed by H NMR spectroscopy combined with multivariate statistical analysis to investigate the metabolomics of RhRR-EuS and RhRR.

KLF8 Promotes the Survival of Lung Adenocarcinoma During Nutrient Deprivation by Regulating the Pentose Phosphate Pathway through SIRT2.

Background: The pentose phosphate pathway (PPP) is a critical metabolic pathway that generates NADPH and ribose-5-phosphate for nucleotide biosynthesis and redox homeostasis. In this study, we investigated a potential regulatory role for Krüppel-like factor 8 (KLF8) in the control of PPP in lung adenocarcinoma (LUAD) cells.

Methods: Based on a comprehensive set of experimental approaches, including cell culture, molecular techniques, and functional assays, we revealed a novel mechanism by which KLF8 promotes the activation of glucose-6-phosphate dehydrogenase (G6PD), a component enzyme in the PPP.

Phosphorylated YBX2 is stabilized to promote glycolysis in brown adipocytes.

Y-box binding protein 2 (YBX2) is an essential modulator of brown adipose tissue activation, yet the regulation on its own expression and the involved mechanism remains largely unknown. Herein, we report the YBX2 protein level, but not mRNA level, is induced in response to acute β-adrenergic signaling. In this context, YBX2 is a dual substrate for both AMPK and Akt2.

Comprehensive Quality Evaluation of and Its Processed Product: Chemical Fingerprinting, Determination and Bioactivity.

Processing of Chinese herbal medicines (CHMs) is a traditional pharmaceutical technology in Chinese medicine. Traditionally, proper processing of CHMs is necessary to meet the specific clinical requirements of different syndromes. Processing with black bean juice is considered one of the most important techniques in traditional Chinese pharmaceutical technology.

Preparation and characterization of zein-lecithin-total flavonoids from complex nanoparticles and the study of their antioxidant activity on HepG2 cells.

Total flavonoids from (TFSG) exhibit several biological activities; however, their poor stability limits their application. In this work, zein-lecithin-TFSG complex nanoparticles (Z-L-TFSG NPs) were prepared using the anti-solvent coprecipitation technique. The prepared Z-L-TFSG NPs were spherical with an encapsulation efficiency of 98.

Metabolic regulation mechanism of extract in rats based on H-NMR metabonomics.

Objective: To establish a metabonomics research technique based on the combination of H-NMR and multivariate statistical analysis, so as to explore the metabolic regulation mechanism of extract (ARCE) in rat tissues and serum.

Methods: SD rats were randomly divided into blank group, female group and male group. The H-NMR technique was used to collect the information of rat tissues and serum samples in each group.

Study on pharmacokinetics and tissue distribution of Polygonatum sibiricum polysaccharide in rats by fluorescence labeling.

To study the pharmacokinetics and tissue distribution characteristics of Polygonatum sibiricum (P. sibiricum) polysaccharide administered orally and intravenously in rats, the latest quantitative analysis method was established where P. sibiricum polysaccharide was labeled with fluorescein isothiocyanate (FITC) in plasma and tissues.

Determination of polysaccharides composition in and by HPLC-FLD with pre-column derivatization.

A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in and . The polysaccharides were de-esterified, extracted, hydrolyzed and derivatized with p-aminobenzoic acid (PABA) to obtain fluorescently labeled monosaccharide compounds, which were finally detected by HPLC-FLD. Inertsil ODS-3, C18 chromatographic column (250 mm × 4.

Limonoids from Swietenia macrophylla and their antitumor activities in A375 human malignant melanoma cells.

Swietelinins A - C (1-3) and swieteliacates F - R (4-16), sixteen new limonoids and 18 known limonoids (17-34) were isolated from Swietenia macrophylla. The absolute configurations of these compounds were defined by using a combination of electronic circular dichroism data analysis and single-crystal X-ray diffraction data. Swieteliacate J (10) is the first limonoid possessing an unusual 8β, 9β-epoxy ring system.

PWWP2B Fine-Tunes Adipose Thermogenesis by Stabilizing HDACs in a NuRD Subcomplex.

Histone deacetylases (HDACs) are widely involved in many biological processes, as well as in control of brown and beige adipose physiology, but the precise molecular mechanisms by which HDACs are assembled into transcriptional machinery to fine-tune thermogenic program remain ill-defined. PWWP domain containing 2b (PWWP2B), which is identified as a component of the nucleosome remodeling and deacetylation complex (NuRD), interacts and stabilizes HDAC1/2 at the thermogenic gene promoters to suppress their expression. Ablation of Pwwp2b promotes adipocyte thermogenesis and ameliorates diet-induced obesity in vivo.

KMT5c modulates adipocyte thermogenesis by regulating expression.

Brown and beige adipocytes harbor the thermogenic capacity to adapt to environmental thermal or nutritional changes. Histone methylation is an essential epigenetic modification involved in the modulation of nonshivering thermogenesis in adipocytes. Here, we describe a molecular network leading by KMT5c, a H4K20 methyltransferase, that regulates adipocyte thermogenesis and systemic energy expenditure.

Kalshiolin A, new lignan from .

The Asian plant has been used as food and ethnologic medicine for over a thousand years. In this study, we isolated and identified one new lignan, kalshiolin A (), and 12 known lignans (). The structures were characterized by the comprehensive analyses of spectroscopic data (HR-ESI-MS, IR, 1D, and 2D-NMR) and the absolute configuration of was determined from ECD calculations.

Arahyside A, a new norsesquiterpene from .

Six compounds were isolated from the stems and leaves of , including a new norsesquiterpene, arahyside A (), and five known compounds, 4-(2-methoxyethyl)benzene-1,2-diol (), (1(,),2(,))-1-phenylpropane-1,2-diol (), tachioside (), 1,3-benzenediol (), demethylmedicarpin (). Extensive spectroscopic methods, containing HR-EI-MS, NMR and ECD were used for structural elucidation of this new compound . Furthermore, its cytotoxicity was evaluated by MTT assay.

Chemical Structures and Biological Activities of Limonoids from the Genus (Meliaceae).

is a genus in the plant family Meliaceae. This genus contains seven to eight known species, found in the tropical and subtropical regions of the Americas and West Africa. Thus far, more than 160 limonoids have been isolated from four species of the genus .

Ifi27 is indispensable for mitochondrial function and browning in adipocytes.

Brown adipose tissue (BAT) is specialized for energy expenditure, but the signaling pathways that regulate BAT metabolism and activity are incompletely understood. Interferon (IFN) signaling is a sophisticated defense mechanism to counteract viral infection. IFNs and interferon-stimulated genes (ISGs) are reported to exert profound effects on adipocytes.

[Role of NO signal in ABA-induced phenolic acids accumulation in Salvia miltiorrhiza hairy roots].

To investigate roles of nitric oxide (NO) signal in accumulations of phenolic acids in abscisic.acid (ABA)-induced Salvia miltiorrhiza hairy roots, S. miltiorrhiza hairy roots were treated with different concentrations of sodium nitroprusside (SNP)-an exogenous NO donor, for 6 days, and contents of phenolic acids in the hairy roots are determined.