Supplementation with NAD+ and its precursors: A rescue of female reproductive diseases.

Nicotinamide adenine dinucleotide (NAD) is an essential coenzyme involved in many pathophysiological processes. Supplementation with NAD and its precursors has been demonstrated as an emerging therapeutic strategy for the diseases. NAD also plays an important role in the reproductive system.

[Correlation between Gene SNP and MTX Resistance in Children with ALL].

Objective: To investigate the correlation between single-nucleotide polymorphism (SNP) of gene and resistance to methotrexate (MTX) in children with acute lymphoblastic leukemia (ALL).

Methods: A total of 144 children with ALL who were treated in General Hospital of Ningxia Medical University from January 2015 to November 2021 were enrolled and divided into MTX resistant group and non-MTX resistant group, with 72 cases in each group. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) technology was used to measure the SNP of gene in all children and analyze its correlation with MTX resistant.

Triggered peroxidase-like activity of Au decorated carbon dots for colorimetric monitoring of Hg enrichment in Chlorella vulgaris.

Developing a rapid, low-cost, and multimode detection method for heavy metal ions remains a compelling goal for many applications, including food safety, environmental and biological analysis. This study investigated the influence of Hg on the peroxidase-like activity of gold nanoparticles (GNPs) decorated on carbon dots (CDs) from lysine (denoted as GNP@CDs). A new type of Hg-triggered peroxidase-like activity of GNP@CDs was discovered, which could catalyze the oxidation of the colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMB.

Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite.

Enzyme-mimicking (nanozyme)-based biosensors are attractive owing to their unique catalytic efficiency, multifunctionality, and tunable activity, but examples of oxidase-like nanozymes are quite rare. Herein, we demonstrated that histidine-capped gold nanoclusters (His@AuNCs) possessed intrinsic oxidase-like activity, which could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to blue colored ox-TMB without HO. The assembly of reduced graphene oxide (RGO) with His@AuNCs could further improve its oxidase-like activity and the His@AuNCs/RGO nanocomposites had a lower Michaelis constant (K) and higher catalytic constant (K) for TMB oxidation.