Application of magnetism in tissue regeneration: recent progress and future prospects.

Tissue regeneration is a hot topic in the field of biomedical research in this century. Material composition, surface topology, light, ultrasonic, electric field and magnetic fields (MFs) all have important effects on the regeneration process. Among them, MFs can provide nearly non-invasive signal transmission within biological tissues, and magnetic materials can convert MFs into a series of signals related to biological processes, such as mechanical force, magnetic heat, drug release, etc.

Biomimetic-inspired piezoelectric ovalbumin/BaTiO scaffolds synergizing with anisotropic topology for modulating Schwann cell and DRG behavior.

The treatment of peripheral nerve injury is a clinical challenge that tremendously affected the patients' health and life. Anisotropic topographies and electric cues can simulate the regenerative microenvironment of nerve from physical and biological aspects, which show promising application in nerve regeneration. However, most studies just unilaterally emphasize the effect of sole topological- or electric- cue on nerve regeneration, while rarely considering the synergistic function of both cues simultaneously.

IKVAV functionalized oriented PCL/FeO scaffolds for magnetically modulating DRG growth behavior.

The re-bridging of the deficient nerve is the main problem to be solved after the functional impairment of the peripheral nerve. In this study, a directionally aligned polycaprolactone/triiron tetraoxide (PCL/FeO) fiber scaffolds were firstly prepared by electrospinning technique, and further then grafted with IKVAV peptide for regulating DRG growth and axon extension in peripheral nerve regeneration. The results showed that oriented aligned magnetic PCL/FeO composite scaffolds were successfully prepared by electrospinning technique and possessed good mechanical properties and magnetic responsiveness.

Cell culture on suspended fiber for tissue regeneration: A review.

Cell culturing is a cornerstone of tissue engineering, playing a crucial role in tissue regeneration, drug screening, and the study of disease mechanisms. Among various culturing techniques, 3D culture systems, particularly those utilizing suspended fiber scaffolds, offer a more physiologically relevant environment than traditional 2D monolayer cultures. These 3D scaffolds enhance cell growth, differentiation, and proliferation by mimicking the in vivo cellular milieu.

A Fast Algorithm for Estimating Two-Dimensional Sample Entropy Based on an Upper Confidence Bound and Monte Carlo Sampling.

The two-dimensional sample entropy marks a significant advance in evaluating the regularity and predictability of images in the information domain. Unlike the direct computation of sample entropy, which incurs a time complexity of O(N2) for the series with length, the Monte Carlo-based algorithm for computing one-dimensional sample entropy (MCSampEn) markedly reduces computational costs by minimizing the dependence on . This paper extends MCSampEn to two dimensions, referred to as MCSampEn2D.

Development of ovalbumin implants with different spatial configurations for treatment of peripheral nerve injury.

Peripheral nerve injury (PNI) seriously affects the health and life of patients, and is an urgent clinical problem that needs to be resolved. Nerve implants prepared from various biomaterials have played a positive role in PNI, but the effect should be further improved and thus new biomaterials is urgently needed. Ovalbumin (OVA) contains a variety of bioactive components, low immunogenicity, tolerance, antimicrobial activity, non-toxicity and biodegradability, and has the ability to promote wound healing, cell growth and antimicrobial properties.

Piezoelectric materials for neuroregeneration: a review.

The purpose of nerve regeneration tissue engineering strategies is to create a microenvironment that mimics natural nerve growth for achieving functional recovery. Biomaterial scaffolds offer a promising option for the clinical treatment of large nerve gaps due to the rapid advancement of materials science and regenerative medicine. The design of biomimetic scaffolds should take into account the inherent properties of the nerve and its growth environment, such as stiffness, topography, adhesion, conductivity, and chemical functionality.

Surface topologized ovalbumin scaffolds containing YIGSR peptides for modulating Schwann cell behavior.

Peripheral nerve injuries (PNI) currently have limited therapeutic efficacy, and functional scaffolds have been shown to be effective for treating PNI. Ovalbumin (OVA) is widely used as a natural biomaterial for repairing damaged tissues due to its excellent biocompatibility and the presence of various bioactive components. However, there are few reports on the repair of PNI by ovalbumin.

Quantitative evaluation of carbon emissions with mining technology development: a case study of an iron mine in China.

To quantitatively evaluate the carbon emission effects of various underground mining schemes in metal mines, a carbon emission calculation model specifically for underground metal mines was established. The carbon emissions stemming from the mine's production process were categorized into three components: carbon emissions from the production of consumed materials, fuel, and electricity; carbon emissions resulting from fuel combustion and explosive explosions, and the reduction of CO absorption due to the occupation of the surface industrial site. Subsequently, the carbon emission impact of underground metal mines was assessed using an example from an iron mine in Anhui Province, China.

Leaching toxicity and deformation failure characteristics of phosphogypsum-based cemented paste backfill under chemical solution erosion.

In order to explore the potential environmental and safety risks of phosphogypsum-based cemented paste backfill (PCPB) in mines, aiming at the actual problems of different acidity and alkalinity of the groundwater environment where PCPB is located, the chemical solution erosion test, element concentration determination test, uniaxial compressive strength (UCS) test, and microscopic analysis test of PCPB were carried out. The effects of three different chemical solutions, HCl solution, NaOH solution, and pure water on the leaching toxicity and deformation failure characteristics of PCPB were analyzed. The kinetic equations of pH value of PCPB in the HCl and NaOH solutions, the leaching models of total P and fluoride, and the UCS erosion model of PCPB were established.

Multi-scale, multi-level anisotropic silk fibroin/metformin scaffolds for repair of peripheral nerve injury.

Silk fibroin (SF) as a natural polymer has a long history of application in various regenerative medicine fields, but there are still many shortcomings in silk fibroin for using as nerve scaffolds, which limit its clinical application in peripheral nerve regeneration (PNR). In this work, a multi-scale and multi-level metformin (MF)-loaded silk fibroin scaffold with anisotropic micro-nano composite topology was prepared by micromolding electrospinning for accelerating PNR. The scaffolds were characterized for morphology, wettability, mechanical properties, degradability, and drug release, and Schwann cells (SCs) and dorsal root ganglia (DRG) were cultured on the scaffolds to assess their effects on neural cell behavior.

Mechanical properties and damage constitutive model of phosphogypsum-based cemented backfill under hydrochemical action.

In order to analyze the early mechanical properties and damage characteristics of phosphogypsum-based cemented backfill (PCB) under hydrochemical action, hydrochemical erosion and uniaxial compression strength (UCS) tests were carried out with HCl solution, NaOH solution, and water respectively. The damage degree is defined by taking the effective bearing area of the soluble cements of PCB under hydrochemistry action as the chemical damage variable, and the modified damage parameter α, which reflects the damage development characteristics, is introduced to construct the damage constitutive model of PCB considering chemical damage and load damage, and the theoretical model is verified with the experimental results. The results show that the damage constitutive model curves of PCB under different hydrochemical action are in good agreement with the experimental results, which verifies the correctness of the theoretical model.

A novel Bursin-like peptide as a potential virus inhibitor and immunity regulator in SPF chickens infected with recombinant ALV.

Background: Avian leukosis viruses (ALVs) are important contagious suppressive factors of chicken immunity and growth performance, resulted in enormous economic loss. Although virus eradication programs are applied in breeder flocks, ALVs are still widespread globally. Therefore, other valuable adjunct to reduce the negative effect of ALVs should be considered.

Effects of Lactobacillus delbrueckii on immune response, disease resistance against Aeromonas hydrophila, antioxidant capability and growth performance of Cyprinus carpio Huanghe var.

The aim of the present study was to investigate effects of dietary Lactobacillus delbrueckii (L. delbrueckii) on immune response, disease resistance against Aeromonas hydrophila (A. hydrophila), antioxidant capability and growth performance of Cyprinus carpio Huanghe var.

Protective effect of reduced glutathione C60 derivative against hydrogen peroxide-induced apoptosis in HEK 293T cells.

Hydrogen peroxide (H2O2) and free radicals cause oxidative stress, which induces cellular injuries, metabolic dysfunction, and even cell death in various clinical abnormalities. Fullerene (C60) is critical for scavenging oxygen free radicals originated from cell metabolism, and reduced glutathione (GSH) is another important endogenous antioxidant. In this study, a novel water-soluble reduced glutathione fullerene derivative (C60-GSH) was successfully synthesized, and its beneficial roles in protecting against H2O2-induced oxidative stress and apoptosis in cultured HEK 293T cells were investigated.

Tributyltin promoted hepatic steatosis in zebrafish (Danio rerio) and the molecular pathogenesis involved.

Endocrine disruptor effects of tributyltin (TBT) are well established in fish. However, the adverse effects on lipid metabolism are less well understood. Since the liver is the predominant site of de novo synthesis of lipids, the present study uses zebrafish (Danio rerio) to examine lipid accumulation in the livers and hepatic gene expression associated with lipid metabolism pathways.

Photodynamic anticancer activities of water-soluble C(60) derivatives and their biological consequences in a HeLa cell line.

Photodynamic therapy is an emerging, externally activatable, treatment modality for various diseases, especially for cancer therapy. The photodynamic activities of tumor targeting water-soluble C(60) derivatives (WSFD) were evaluated on HeLa cells. To overcome the poor solubility, biocompatibility and selectivity of C(60), we modified C(60) with l-phenylalanine, folic acid and l-arginine.

Protective effect of C(60) -methionine derivate on lead-exposed human SH-SY5Y neuroblastoma cells.

Oxidative stress has been considered as one of the possible mechanisms leading to the neurotoxicity of lead. One of the effective ways to prevent cellular damage after lead exposure is using antioxidants. In this paper, a novel C(60) -methionine derivate (FMD), a fullerene molecule modified with methionine, was synthesized.

The protective activities of water-soluble C(60) derivatives against nitric oxide-induced cytotoxicity in rat pheochromocytoma cells.

In this study, the protective activities of water-soluble C(60) derivatives against nitric oxide (NO) induced cytotoxicity were investigated. To overcome C(60) insolubility in water, we modified C(60) with β-alanine, valine or folacin. The compounds were characterized by FT-IR, (1)H NMR, (13)C NMR, LC-MS, elemental analysis, light scattering and TEM.

Folacin C60 derivative exerts a protective activity against oxidative stress-induced apoptosis in rat pheochromocytoma cells.

In the present study, we describe the synthesis and characterization of a novel folacin C(60) derivative. The compound was analyzed by FT-IR, (1)H NMR, (13)C NMR, LC-MS and elemental analysis. This water soluble fullerene derivative was able to scavenge both superoxide and hydroxyl radical with biocompatibility.

Synthesis of glutathione C60 derivative and its protective effect on hydrogen peroxide-induced apoptosis in rat pheochromocytoma cells.

Oxidized glutathione C(60) derivative has been synthesized and characterized in our research. As a novel derivative of C(60), the glutathione C(60) derivative is soluble in dimethylsulfoxide, dimethylformamide and dimethylacetamide. Rat pheochromocytoma (PC12) cells are treated with hydrogen peroxide and underwent cytotoxicity; apoptotic death is determined by MTT assay, flow cytometry analysis, PI/Hoechst 33342 staining and glutathione assay.

Protective effect of a novel cystine C(60) derivative on hydrogen peroxide-induced apoptosis in rat pheochromocytoma PC12 cells.

Oxidative stress has been considered as a major cause of cellular injuries in a variety of clinical abnormalities, especially neural diseases. One of the effective ways to prevent the reactive oxygen species (ROS) mediated cellular injury is dietary or pharmaceutical augmentation of free radical scavengers. In the present study, we describe the synthesis and characterization of a novel cystine C(60) derivative (CFD).

Synthesis of beta-alanine C60 derivative and its protective effect on hydrogen peroxide-induced apoptosis in rat pheochromocytoma cells.

Oxidative stress has been considered as a major cause of cellular injuries in a variety of clinical abnormalities, especially prominent in neural diseases. One of the effective ways to prevent the reactive oxygen species (ROS) mediated cellular injury is dietary or pharmaceutical augmentation of some free radical scavenger. Water-soluble amino-fullerene derivative is a novel compound that behaves as a free radical scavenger with excellent biocompatibility.

Covalent functionalization of multiwalled carbon nanotubes with a low molecular weight chitosan.

Covalent functionalization of shortened multiwalled carbon nanotubes (MWNTs) with a natural low molecular weight chitosan (LMCS) was accomplished by a nucleophilic substitution reaction. Amino and primary hydroxyl groups of the LMCS contributed mainly to the formation of MWNT-LMCS conjugates. The LMCS content in the MWNT-LMCS is approximately 58 wt %, and approximately four molecular chains of the LMCS are attached to 1000 carbon atoms of the nanotube sidewalls.