Acetylation of PGK1 at lysine 323 promotes glycolysis, cell proliferation, and metastasis in luminal A breast cancer cells.

Background: In prior research employing iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) technology, we identified a range of proteins in breast cancer tissues exhibiting high levels of acetylation. Despite this advancement, the specific functions and implications of these acetylated proteins in the context of cancer biology have yet to be elucidated. This study aims to systematically investigate the functional roles of these acetylated proteins with the objective of identifying potential therapeutic targets within breast cancer pathophysiology.

Systematic proteomics analysis revealed different expression of laminin interaction proteins in breast cancer: lower in luminal subtype and higher in claudin-low subtype.

Background: Breast cancer is a major public health concern. Proteomics enables identification of proteins with aberrant properties. Here, we identified proteins with abnormal expression levels in breast cancer tissues and systematically analyzed and validated the data to locate potential diagnostic and therapeutic targets.

Role in posttranslational modification of M2type pyruvate kinase in tumorigenesis and development.

PKM2, also known as M2-type pyruvate kinase, has attracted significant attention due to its crucial role in glycolysis and its abnormal expression in various tumors. With the discovery of PKM2's non-metabolic functions, the transition between its pyruvate kinase activity (in the tetrameric form in the cytoplasm) and protein kinase activity (in the dimeric form in the nucleus) has once again made PKM2 a target of interest in cancer research. Studies have shown that PKM2 is a protein susceptible to various post-translational modifications, and different post-translational modifications play important regulatory roles in processes such as PKM2 cellular localization, structure, and enzyme activity conversion.

Precise isolation and structural origin of an ultra-specific nanobody against chemical compound.

Highly specific antibodies are the key reagents for developing immunoassays with a low false positive rate for environmental monitoring. Here, we provide evidence that nanobodies have the potential to achieve higher specificity than conventional antibodies and explain why from their structural features. Using sulfadimethoxine (SDM) as a model analyte, we constructed an immune phage display library and precisely isolated an ultra-specific nanobody (H1-17) by a crucial homologous antigen counter selection strategy.

Functional characterization of knockdown resistance mutations in the plant bug, Apolygus lucorum Meyer-Dür.

Apolygus lucorum could cause severe economic damage to crops in China. The pest has been controlled by pyrethroids, and the target of pyrethroids is voltage-gated sodium channel (Na). Double mutation (LF/DG) was detected in a field-strain of A.

Unique post-translational modifications diversify the sodium channels in peach aphid (Myzus persicae [Sulzer]).

Background: Myzus persicae (Sulzer), a worldwide pest, has caused remarkable damage to agriculture. Among the various control methods, chemical control (especially pyrethroids) is most commonly used. The targets of pyrethroids are voltage-gated sodium channels (Na s).

A Preliminary Toxicology Study on Eco-friendly Control Target of Spodoptera frugiperda.

Pyrethroid and indoxacarb are commonly used pesticides to control the fall armyworm (Spodoptera frugiperda) in the crops. There are a series of consequences caused by the use of pyrethroid and indoxacarb pesticides under emergency control, such as pest resistance development, contamination of soil, water, and farm products. This study analyzed the structure and physiological function of the sodium channel in S.

Two classic mutations in the linker-helix IIL45 and segment IIS6 of Apolygus lucorum sodium channel confer pyrethroid resistance.

Background: Pyrethroids are classified as type I and type II for distinct symptomology. Voltage-gated sodium channel is a primary target of pyrethroids. Mutations of the insect sodium channel have been identified to result in resistance to pyrethroids.

Molecular characterization and functional expression of voltage-gated sodium channel variants in Apolygus lucorum (Meyer-Dür).

Background: Apolygus lucorum (Meyer-Dür) is a serious worldwide agricultural pest, especially for Bt cotton in China. Pyrethroids, neonicotinoids and organophosphates are the most effective insecticides to control piercing and sucking insects, including A. lucorum.

Assessment of the effects of lethal and sublethal exposure to dinotefuran on the wheat aphid Rhopalosiphum padi (Linnaeus).

The wheat aphid Rhopalosiphum padi (Linnaeus) (Hemiptera: Aphididae) is a devastating pest of wheat crops worldwide. Dinotefuran, a novel neonicotinoid insecticide, has been used to prevent piercing-sucking agricultural insects, such as R. padi.

Genome Analysis of Cytochrome in Dinotefuran-Treated Apolygus lucorum (Meyer-Dür).

In this study, two CYP genes, CYP395G1 and CYP4EY1, were analyzed in Apolygus lucorum (Hemiptera: Miridae). The expression pattern in different developmental stages of both CYP395G1 and CYP4EY1 revealed that first instar nymphs possessed the highest gene-transcript levels. After 12 h of dinotefuran treatments, the expression levels of CYP395G1 increased by 1.

Structural Health Monitoring of Above-Ground Storage Tank Floors by Ultrasonic Guided Wave Excitation on the Tank Wall.

There is an increasing interest in using ultrasonic guided waves to assess the structural degradation of above-ground storage tank floors. This is a non-invasive and economically viable means of assessing structural degradation. Above-ground storage tank floors are ageing assets which need to be inspected periodically to avoid structural failure.

On the scattering of torsional waves from axisymmetric defects in buried pipelines.

This article develops a numerical model suitable for analysing elastic wave scattering in buried pipelines. The model is based on a previous so-called hybrid approach, where a nominally infinite length of pipe is split up into uniform and non-uniform regions. The key challenge for buried structures is in enforcing the appropriate boundary conditions in both the axial and radial directions, which must encompass the entire length of the structure, as well as the surrounding material.

[Clinical analysis of difficult intraarterial mechanical thrombectomy in patients with acute ischemic stroke].

Objective: To investigate the causes and strategy of difficult intraarterial mechanical thrombectomy (≥3 times) in patients with acute ischemic stroke (AIS).

Methods: The clinical data of 8 cases of AIS with thrombectomy ≥3 times admitted in Department of Neurology, the 306(th) Hospital of People's Liberation Army from June to October in 2015 was analyzed retrospectively. There were 7 male and 1 female patients, aged from 38 to 86 years with an average age of (70±15) years, in which 5 cases were cardiogenic cerebral embolism and 3 cases were large artery atherosclerotic infarction.

On the scattering of elastic waves from a non-axisymmetric defect in a coated pipe.

Viscoelastic coatings are often used to protect pipelines in the oil and gas industry. However, over time defects and areas of corrosion often form in these pipelines and so it is desirable to monitor the structural integrity of these coated pipes using techniques similar to those used on uncoated pipelines. A common approach is to use ultrasonic guided waves that work on the pulse-echo principle; however, the energy in the guided waves can be heavily attenuated by the coating and so significantly reduce the effective range of these techniques.

Measurement of complex acoustic intensity in an acoustic waveguide.

Acoustic intensity is normally treated as a real quantity, but in recent years, many articles have appeared in which intensity is treated as a complex quantity where the real (active) part is related to local mean energy flow and the imaginary (reactive) part to local oscillatory transport of energy. This offers the potential to recover additional information about a sound field and then to relate this to the properties of the sound source and the environment that surrounds it. However, this approach is applicable only to multi-modal sound fields, which places significant demands on the accuracy of the intensity measurements.

A hybrid finite element approach to modeling sound radiation from circular and rectangular ducts.

A numerical model based on a hybrid finite element method is developed that seeks to join sound pressure fields in interior and exterior regions. The hybrid method is applied to the analysis of sound radiation from open pipes, or ducts, and uses mode matching to couple a finite element discretization of the region surrounding the open end of the duct to wave based modal expansions for adjoining interior and exterior regions. The hybrid method facilitates the analysis of ducts of arbitrary but uniform cross section as well the study of conical flanges and here a modal expansion based on spherical harmonics is applied.