The low grade of biogas and the difficulty of treating biogas slurry are the two major bottlenecks limiting the sustainable development of the fermentation engineering. This study investigates the potential role of microalgae-microbial symbiosis and phytohormones in solving this challenge. Chlorella microalgae were combined with endophytic bacteria (S395-2) and Clonostachys fungus to construct symbiotic systems.
View Article and Find Full Text PDFSludge alkaline fermentation liquid (SAFL) is an alternative to sodium acetate (NaAc) in enhancing wastewater nitrogen removal. Upon SAFL addition, dissolved organic nitrogen (DON) can be externally introduced or biologically synthesized during nitrogen removal, which is an important precursor to toxic nitrogenous disinfection by-products (N-DBPs). This study aims to evaluate the effects of different carbon source addition on effluent DON concentration, composition, and N-DBP formation potentials.
View Article and Find Full Text PDFThe emergence of antibiotic resistance in retort to environmental pollutants during wastewater treatment still remains elusive. Here, we first to investigate the emergence of antibiotic resistance in an environmental non-pathogenic bacterium, Pseudoxanthomonas mexicana isolated from a lab-scale bioreactor treating wastewater containing streptomycin. The molecular mechanism of antibiotic resistance development was evaluated in its genomic, transcriptional, and proteomic levels.
View Article and Find Full Text PDFThe application of mainstream anammox process is hampered by its overlong start-up and instability under disturbance. A lab-scale mainstream anammox moving bed biofilm reactor (MBBR) was successfully started in 120 days with stepwise decrement in influent nitrogen concentration from sidestream to mainstream condition. The initial colonization by Candidatus Jettenia and filamentous fermenter Anaerolineaceae were potentially mediated by hydrophobic interaction and type IV pilus.
View Article and Find Full Text PDFMicroRNAs (miRNAs) are small, non-coding regulatory RNAs that regulate gene expression by facilitating target mRNA cleavage in plants. They are crucial for responses to diverse stresses. The novel drought-responsive miRNA ZmmiR190 was previously identified during an analysis of the maize transcriptome.
View Article and Find Full Text PDFComplete ammonia oxidizing (comammox) bacteria are frequently detected in wastewater biological nutrient removal (BNR) systems. This study identified "Candidatus Nitrospira nitrosa"-like comammox bacteria as the predominant ammonia oxidizers (97.5-99.
View Article and Find Full Text PDFWith the upgrade of wastewater treatment plants (WWTPs) to meet more stringent discharge limits for nutrients, dissolved organic nitrogen (DON) is present at an increasing percentage (up to 85%) in the effluent. Discharged DON is of great environmental concern due to its potentials in stimulating algal growth and forming toxic nitrogenous disinfection by-products (N-DBPs). This article systematically reviewed the characteristics, transformation and ecological impacts of wastewater DON.
View Article and Find Full Text PDFNucleobase:cation symporter 2 (NCS2) proteins are important for the transport of free nucleobases, participating in diverse plant growth and developmental processes, as well as response to abiotic stress. To date, a comprehensive analysis of the gene family has not been performed in maize. In this study, we conducted a comparative genomics analysis of genes in 28 plant species, ranging from aquatic algae to land plants, concentrating mainly on maize.
View Article and Find Full Text PDFHD-Zip proteins represent the major transcription factors in higher plants, playing essential roles in plant development and stress responses. Foxtail millet is a crop to investigate the systems biology of millet and biofuel grasses and the HD-Zip gene family has not been studied in foxtail millet. For further investigation of the expression profile of the HD-Zip gene family in foxtail millet, a comprehensive genome-wide expression analysis was conducted in this study.
View Article and Find Full Text PDFThe family is a group of plant-specific transcription factors. genes encode proteins harboring bHLH structure, which is implicated in DNA binding and protein-protein interactions and known as the TCP domain. TCP genes play important roles in plant development and have been evolutionarily and functionally elaborated in various plants, however, no overall phylogenetic analysis or expression profiling of genes in has been reported.
View Article and Find Full Text PDFBacillus amyloliquefaciens FZB42 is a type of plant growth-promoting rhizobacterium (PGPR) which activates induced systemic resistance (ISR) in Arabidopsis. Blocking of the synthesis of cyclic lipopeptides and 2,3-butanediol by FZB42, which have been demonstrated to be involved in the priming of ISR, results in the abolishment of the plant defence responses. To further clarify the ISR activated by PGPRs at the microRNA (miRNA) level, small RNA (sRNA) libraries from Arabidopsis leaves after root irrigation with FZB42, FZB42ΔsfpΔalsS and control were constructed and sequenced.
View Article and Find Full Text PDFNAC transcription factors comprise a large plant-specific gene family. Increasing evidence suggests that members of this family have diverse functions in plant growth and development. In this study, we performed a genomewide survey of NAC type genes in maize (Zea mays L.
View Article and Find Full Text PDFPomelo peel (PP), as one of the well-known agricultural wastes, is cost-effective and environmentally friendly. Based on PP, two new kinds of oil sorbents were prepared by using acetic anhydride and styrene. The structures of raw pomelo peel (RP), acetic anhydride-treated pomelo peel (AP) and styrene-treated pomelo peel (SP) were characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM), contact-angle (CA) measurements.
View Article and Find Full Text PDFMicroRNAs (miRNAs) are a class of small, non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition in plants and animals. At present there is relatively little information regarding the role of miRNAs in the response to drought stress in maize. In this study, two small RNA libraries were sequenced, and a total of 11,973,711 and 14,326,010 raw sequences were generated from growing leaves of drought-tolerant and drought-sensitive maize seedlings, respectively.
View Article and Find Full Text PDFQuinazoline derivatives have wide biological activities and therapeutic properties, implying their potential for development and application on a large scale. In the present study, 2-(4-chlorophenyl)-4-(4-methoxyphenyl) quinazoline (CMQ), was selected to examine its effect on unicellular cyanobacteria, Microcystis aeruginosa by evaluating growth, physiological and molecular responses. Growth was inhibited by CMQ, with a 96 h EC50 of 1.
View Article and Find Full Text PDFGenetic diversity and differential microcystin contributions within the populations of toxic Microcystis in freshwater ecosystems have not been fully discussed. To illustrate this issue, we sequenced clone libraries of the first adenylation domain of mcyB (mcyBA1) from Microcystis populations in a plateau lake. Phylogenetic analysis revealed two divergent groups of McyBA1 with two closely related subgroups within Group I.
View Article and Find Full Text PDFScirpus triqueter has been reported to be an effective phytoremediation plant for pyrene dissipation. The study of S. triqueter in response to pyrene is crucial to understand the mechanism of phytoremediation.
View Article and Find Full Text PDFACS Appl Mater Interfaces
August 2013
The preparation, by a freeze-thaw method, of new graphite/isobutylene-isoprene rubber (IIR) sorbents for oil and organic liquid is described. Graphite was expected to improve the adsorption properties. The cryogels were prepared by solution crosslinking IIR rubber in the presence of graphite in benzene at various temperatures, using sulfur monochloride as the crosslinker, and characterized by SEM and contact angle measurements.
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