To explore the molecular targets for regulating glucose metabolism in carnivorous fish, the turbot () was selected as the research object to study. Farnesoid X receptor (FXR; NR1H4), as a ligand-activated transcription factor, plays an important role in glucose metabolism in mammals. However, the mechanisms controlling glucose metabolism mediated by FXR in fish are not understood.
View Article and Find Full Text PDFThe aim of the present study was to investigate the influence of supplementation of lysozyme (LZM) into diet on the growth performance, muscle quality, immunity, intestinal microbiota in large yellow croaker (Larimichthys crocea) with initial body weight of 194.5 ± 0.27 g.
View Article and Find Full Text PDFBackground: As one of the malignant tumors with the highest incidence and fatality in the world, colon adenocarcinoma (COAD) has a very complex pathogenic mechanism, which has not yet been fully elucidated. Ubiquitin can regulate cell proliferation, cell cycle, apoptosis, DNA damage repair, and other processes by changing the activity of substrate proteins or causing ubiquitin-proteasome degradation. These are the key links in the pathogenesis of COAD, and ubiquitin plays an important role in the occurrence and development of COAD.
View Article and Find Full Text PDFBackground: It has been established that Spalt-Like Transcription Factor 4 (SALL4) promotes Colorectal Cancer (CRC) cell proliferation. Furthermore, Amphiregulin (AREG) is crucially involved in cancer cell proliferation and therapeutic resistance regulation. In this regard, this study aimed to establish whether SALL4 affects the radiosensitization of CRC cells via AREG expression regulation.
View Article and Find Full Text PDFRNA pseudoknots are RNA molecules with specialized three-dimensional structures that play important roles in various biological processes. To understand the functions and mechanisms of pseudoknots, it is essential to elucidate their structures and folding pathways. The most fundamental step in RNA folding is the opening and closing of a base pair.
View Article and Find Full Text PDFThe insufficient antioxidant reserves in tumor cells play a critical role in reactive oxygen species (ROS)-mediated therapeutics. Metallothionein-2 (MT-2), an intracellular cysteine-rich protein renowned for its potent antioxidant properties, is intricately involved in tumor development and correlates with a poor prognosis. Consequently, MT-2 emerges as a promising target for tumor therapy.
View Article and Find Full Text PDFPlant Cell Environ
November 2024
Thrips, Frankliniella intonsa, is a highly polyphagous pest with a worldwide distribution. F. intonsa-infested sunflower seeds show marked visual damage.
View Article and Find Full Text PDFClostridium autoethanogenum protein (CAP) is an eco-friendly protein source and has great application potential in aquafeeds. The present study aimed to investigate the effects of dietary CAP inclusion on the anti-oxidation, immunity, inflammation, disease resistance and gut microbiota of abalone Haliotis discus hannai after a 110-day feeding trial. Three isonitrogenous and isolipidic diets were formulated by adding 0 % (control), 4.
View Article and Find Full Text PDFUp to now, it has been believed that invertebrates are unable to synthesize ascorbic acid (AA) in vivo. However, in the present study, the full-length CDs (Coding sequence) of L-gulonolactone oxidase (GLO) from Pacific abalone (Haliotis discus hannai Ino) were obtained through molecular cloning. The Pacific abalone GLO contained a FAD-binding domain in the N-termination, and ALO domain and conserved HWAK motif in the C-termination.
View Article and Find Full Text PDFGenes (Basel)
December 2023
(Joannis) is a pest species with serious outbreaks in the Inner Mongolian grasslands in recent years, and its larvae and eggs are extremely cold-tolerant. To gain a deeper understanding of the molecular mechanism of its cold-tolerant stress response, we performed de novo transcriptome assembly of via RNA-Seq and compared the differentially expressed genes (DEGs) of first- and second-instar larvae grown and developed indoors and outdoors, respectively. The results show that cold tolerance in is associated with changes in gene expression mainly involved in the glycolysis/gluconeogenesis pathway, the fatty acid biosynthesis pathway and the production of heat shock proteins (HSPs).
View Article and Find Full Text PDFThis paper considers the aperiodic intermittent control (AIC) for linear time-varying systems (LTVSs), where the occurrence instants are determined by an event triggering mechanism based on Lyapunov functions. For LTVSs, most of the existing results are demanded that the feedback controls are exerted all the time. In fact, in many practical applications, the applied controls are unnecessary/impossible to be imposed all the time.
View Article and Find Full Text PDFIn this paper, we use a pair of self-resonating subwavelength spoof plasmonic structures to achieve remote non-radiative terahertz wireless power transfer, while nearly without affecting the electromagnetic environment of free space around the structure. The resonating frequency and quality factor of the magnetic dipole mode supported by the spoof plasmonic structures can be freely tuned by tailoring the geometric structure. By putting the weak source and detector into the self-resonating structures, we can find that the effective non-radiative terahertz power transferring distance can reach several hundred times the radius of the structures.
View Article and Find Full Text PDFCopper (Cu) is a trace element, essential for fish growth. In the current study, in addition to growth performance, we first explored the effects of Cu on collagen synthesis and myofiber growth and development in juvenile grass carp (. A total of 1080 fish (11.
View Article and Find Full Text PDFAbalone Haliotis discus hannai (initial weight: 38.79 ± 0.70 g) was used as the experimental animal in a 105-day feeding trial to investigate the influence of dietary bile acids levels on the growth, anti-oxidation, immune response and intestinal microbiota.
View Article and Find Full Text PDFThe use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture.
View Article and Find Full Text PDFComp Biochem Physiol Part D Genomics Proteomics
September 2023
The present study explored transcriptomics and gene regulation variations in the muscle of turbot fed with dietary taurine. A 70-day feeding trial was conducted using turbot (initial body weight: 3.66 ± 0.
View Article and Find Full Text PDFThe presence of internal fissures holds immense sway over the gas permeability of sustainable cement mortar, which in turn dictates the longevity and steadfastness of associated edifices. Nevertheless, predicting the gas permeability of sustainable cement mortar that contains internal cracks poses a significant challenge due to the presence of numerous influential variables and intricate interdependent mechanisms. To solve the deficiency, this research establishes an innovative machine learning algorithm via the integration of the Mind Evolutionary Algorithm (MEA) with the Adaptive Boosting Algorithm-Back Propagation Artificial Neural Network (ABA-BPANN) ensemble algorithm to predict the gas permeability of sustainable cement mortar that contains internal cracks, based on the results of 1452 gas permeability tests.
View Article and Find Full Text PDFTher Clin Risk Manag
July 2023
Accumulation of aberrant proteins in the heart causes cardiac amyloidosis, an uncommon and complicated illness. It can be classified into two main types: light chain (AL) and transthyretin (ATTR). The diagnosis of cardiac amyloidosis is challenging due to its non-specific clinical presentation and lack of definitive diagnostic tests.
View Article and Find Full Text PDFBlood flow and pressure calculated using the currently available methods have shown the potential to predict the progression of pathology, guide treatment strategies and help with postoperative recovery. However, the conspicuous disadvantage of these methods might be the time-consuming nature due to the simulation of virtual interventional treatment. The purpose of this study is to propose a fast novel physics-based model, called FAST, for the prediction of blood flow and pressure.
View Article and Find Full Text PDFCRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated protein (Cas9) has been widely used for gene editing. Not all guide RNAs can cleave the DNA efficiently remains a major challenge to CRISPR/Cas9-mediated genome engineering. Therefore, understanding how the Cas9 complex successfully and efficiently identifies specific functional targets through base-pairing has great implications for such applications.
View Article and Find Full Text PDFIn order to investigate the mechanism of glucagon regulation of gluconeogenesis, primary hepatocytes of the Japanese flounder () were incubated with synthesized glucagon, and methods based on inhibitors and gene overexpression were employed. The results indicated that glucagon promoted glucose production and increased the mRNA levels of glucagon receptor (), guanine nucleotide-binding protein Gs α subunit (), adenylate cyclase 2 (), protein kinase A (), cAMP response element-binding protein 1 (), peroxisome proliferator-activated receptor-γ coactivator 1α (), phosphoenolpyruvate carboxykinase 1 (), and glucose-6-phosphatase () in the hepatocytes. An inhibitor of GCGR decreased the mRNA expression of , , , , , , , the protein expression of phosphorylated CREB and PGC-1α, and glucose production.
View Article and Find Full Text PDFMechanical force has been widely used to study RNA folding and unfolding. Understanding how the force affects the opening and closing of a single base pair, which is a basic step for RNA folding and unfolding and a fundamental behavior in some important biological activities, is crucial to understanding the mechanism of RNA folding and unfolding under mechanical force. In this work, we investigated the opening and closing process of an RNA base pair under mechanical force with constant-force stretching molecular dynamics simulations.
View Article and Find Full Text PDFLiver hepatocellular carcinoma (LIHC) is one of the most common malignancies and places a heavy burden on patients worldwide. HAUS augmin-like complex subunit 5 (HAUS5) is involved in the occurrence and development of various cancers. However, the functional role and significance of HAUS5 in LIHC remain unclear.
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