J Exp Zool A Ecol Integr Physiol
March 2025
In the integrated rice-crayfish aquaculture systems, crayfish Procambarus clarkii need to spend a long winter in the ring ditch, which is vulnerable to low temperature stress, especially in the northern part of China, where cold waves and other low-temperature climates are frequent. To study the metabolic response of P. clarkii to low temperature stress experimentally, the temperature was lowered from the control (23°C) to the low temperature group (9°C, 5°C, and 1°C) by slow and uniform cooling, and molecular and physiological samples were collected for measurement.
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