Optimization of Ultrasonic-Assisted Extraction, Characterization and Antioxidant and Immunoregulatory Activities of Polysaccharides.

This study aimed to enhance the ultrasonic-assisted extraction (UAE) yield of seawater polysaccharides (APPs) and investigate its structural characteristics and bioactivities. The optimization of UAE achieved a maximum crude polysaccharides yield of 14.78%.

Isolation, Characterization, Moisturization and Anti-HepG2 Cell Activities of a Novel Polysaccharide from .

Polysaccharides from cyanobacteria are extensively reported for their complex structures, good biocompatibility, and diverse bioactivities, but only a few cyanobacterial species have been exploited for the biotechnological production of polysaccharides. According to our previous study, the newly isolated marine cyanobacterium SCSIO-45682 was a good candidate for polysaccharide production. This work provided a systematic study of the extraction optimization, isolation, structural characterization, and bioactivity evaluation of polysaccharides from SCSIO-45682.

Multi-Omics Reveals the Effects of Powder Replacement of Fish Meal on Intestinal Metabolism and Stress in Zig-Zag Eel ().

The booming aquaculture industry has created a strong demand for fishmeal and increased environmental pressures. Spirulina, as a potential alternative to fishmeal, has been shown to have growth-promoting and animal health-enhancing properties. In this study, 600 large spiny loaches, divided into five experimental groups, F0, F1, F2, F3, and F4, were reared for 10 weeks using powder (SPP) as a substitute for 0%, 5%, 10%, 15%, and 20% of fishmeal, respectively.

Reconstruction of Long-Chain Polyunsaturated Acid Synthesis Pathways in Marine Red Microalga Using Lipidomics and Transcriptomics.

The marine red microalga can simultaneously synthesize long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (C20:5, EPA) and arachidonic acid (C20:4, ARA). However, the distribution and synthesis pathways of EPA and ARA in are not clearly understood. In this study, CCALA 415 was cultured in nitrogen-replete and nitrogen-limited conditions.

Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants.

In order to rapidly screen microalgae species as feedstocks for antioxidants, extracts were obtained from 16 microalgae strains (under 11 genera, 7 classes) using two methods: a one-step extraction with ethanol/water and a three-step fractionating procedure using hexane, ethylacetate, and water successively. Measuring the total phenol content (TPC), total carotenoid content (TCC), and antioxidant activity of the extracts, indicating TPC and TCC, played an important role in determining the antioxidant activity of the microalgae. A weighted scoring system was used to evaluate the antioxidant activity, and the scores of microalgal samples from two extraction methods were calculated using the same system.

Regulatory effects of marine polysaccharides on gut microbiota dysbiosis: A review.

The gut microbiota dysbiosis is a state which the physiological combinations of flora are transformed into pathological combinations caused by factors such as diets, pollution, and drugs. Increasing evidence shows that dysbiosis is closely related to many diseases. With the continuous development and utilization of marine resources, marine polysaccharides have been found to regulate dysbiosis in many studies.

Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of .

Phycoerythrin, a special photosynthetic pigment, is widely used as fluorescent dye and has lots of underlying beneficial effects on health. A marine red microalga is considered as the potential feedstock for phycoerythrin production. However, the phycoerythrin-related properties of have not been systematically evaluated, especially between the species of and .

Exopolysaccharides from the Energy Microalga Strain : Purification, Characterization, and Antioxidant Activity.

 , a prestigious energy microalga, has recently received widespread attention because it can secrete large amounts of exopolysaccharides (EPS) with potential applications in food, cosmetics, and nutraceuticals. Unfortunately, the insufficiency of research on the bioactivity and structure-activity relationship of . EPS has impeded the downstream applications.

Growth and Biochemical Composition Characteristics of Induced by Simultaneous Nitrogen Deficiency and Seawater-Supplemented Medium in an Outdoor Raceway Pond in Winter.

, a well-known cyanobacterium, is widely applied not only in human and animal nutrition but also in cosmetics for its high amounts of active products. The biochemical composition plays a key role in the application performance of the biomass. The present study aimed to evaluate the growth and biochemical composition characteristics of , cultured with a nitrogen-free and seawater-supplemented medium in an outdoor raceway pond in winter.

Extraction optimization, physicochemical characterization, and antioxidant activity of polysaccharides from sp. SCSIO-45730.

Unlabelled: Microalgal polysaccharides have been reported in many studies due to their uniqueness, biocompatibility, and high value, and sp. SCSIO-45730 was an excellent source of polysaccharides and β-glucans. However, the polysaccharides from the red unicellular alga sp.

A Novel Three-Step Extraction Strategy for High-Value Products from Red Algae .

The microalga accumulates high-value compounds such as phycoerythrin, polyunsaturated fatty acids, and polysaccharides, and thus, the extraction of these compounds could significantly expand the value of biomass. In the present study, a novel fractional extraction strategy based on the characteristics of these compounds was established using cold water, 95% ethanol, and hot water. The yield of phycoerythrin, lipids, and polysaccharides was 63.

Inhibition of enterovirus 71 infection by polysaccharides extracted from Picochlorum sp. 122 via the AKT and ATM/ATR signaling pathways.

Enterovirus 71 (EV71) poses a major threat to public health globally due to severe and even fatal hand, foot, and mouth disease (HFMD). However, no effective antiviral agents are available to treat HFMD caused by this virus. Polysaccharides have been shown to exhibit antiviral activity, and polysaccharides extracted from Picochlorum sp.

Antiaging compounds from marine organisms.

Marine organisms have received mounting attention in antiaging activity due to their exclusive chemicals. This review aims at revealing and discussing prospective antiaging substance from marine macroalgae, micaroalgae, invertebrate and vertebrate. The activity and mechanism of the carbohydrate, protein, pigment, flavonoids, fatty acids, phenols from marine organisms were revealed through a variety of antiaging experimental models such as rats, Drosophila melanogaster and Caenorhabditis elegans.

The inhibition of enterovirus 71 induced apoptosis by Durvillaea antarctica through P53 and STAT1 signaling pathway.

The infection of enterovirus 71 (EV71) resulted in hand, foot, and mouth disease and may lead to severe nervous system damage and even fatalities. There are no effective drugs to treat the EV71 virus and it is crucial to find novel drugs against it. Polysaccharide isolated from Durvillaea antarctica green algae has an antiviral effect.

Growth and Biochemical Composition of SCS-02 under Different Nitrogen Concentrations.

Microalgae of the genus show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce biomass rich in B-PE, LC-PUFAs and EPS. SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.

Induction of Apoptosis in Human Glioma Cells by Fucoxanthin via Triggering of ROS-Mediated Oxidative Damage and Regulation of MAPKs and PI3K-AKT Pathways.

Fucoxanthin, a natural carotenoid derived from algae, exhibits novel anticancer potential. However, fucoxanthin with high purity is hard to prepare, and the anticancer mechanism remains elusive. In the present study, fucoxanthin with high purity was prepared and purified from the marine microalgae Nitzschia sp.

Transcriptome analysis for phosphorus starvation-induced lipid accumulation in Scenedesmus sp.

Phosphorus stress was applied to enhance the lipid production in Scenedesmus sp. The highest lipid production (350 mg L) and lipid content (approximately 41.0% of dry weight) were obtained by addition of 2 mg L NaHPO·2HO every 2 days, which were higher than those in replete phosphorus.

Pelagibacterium lentulum sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122.

A Gram-stain-negative, motile, non-spore-forming, rod-shaped bacterium, designated strain B2T, was isolated from the culture broth of a marine microalga, Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B2T forms a robust cluster with members of the genus Pelagibacterium, and shares the highest sequence similarity of 96.

Butyratibacter algicola gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122.

A Gram-stain-negative, motile, non-spore-forming, ovoid or rod-shaped bacterium, designated strain B15T, was isolated from the culture broth of a marine microalga, Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B15T forms a stable cluster with Lutibaculum baratangense KCTC 22669T (95.

A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock.

Microalgae oil is an optimal feedstock for nutraceutical, pharmaceutical and biodiesel production, but its high levels of chlorophyll limit its large-scale application. To date, few effective approaches have been developed to remove chlorophyll from microalgae oil. The main purpose of this study was to present a preprocessing method of algae oil feedstock (Scenedesmus) to remove chlorophyll by saponification.

Gelatiniphilus marinus gen. nov., sp. nov., a bacterium from the culture broth of a microalga, Picochlorum sp. 122, and emended description of the genus Hwangdonia.

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterium, designated strain GYP-24T, was isolated from the culture broth of a marine microalga, Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GYP-24T forms a robust cluster with H.

Pyruvatibacter mobilis gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122.

A Gram-stain-negative, aerobic bacterium, designated strain GYP-11T, was isolated from the culture broth of a marine microalga, Picochloruma sp. 122. Cells were dimorphic rods; free living cells were motile by means of a single polar flagellum, and star-shaped-aggregate-forming cells were attached with stalks and non-motile.

Aliikangiella marina gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122, and proposal of Kangiellaceae fam. nov. in the order Oceanospirillales.

A Gram-stain-negative, non-motile, non-spore-forming, long rod-shaped bacterium, designated strain GYP-15T, was isolated from the culture broth of a marine microalga, Picochloruma sp. 122. Phylogenetic analyses revealed that strain GYP-15T shared 90.

Oceanobacillus bengalensis sp. nov., a bacterium isolated from seawater of the Bay of Bengal.

A Gram-stain positive, motile, and subterminal endospore-forming rod-shaped bacterium, designated strain Ma-21(T), was isolated from seawater of the Bay of Bengal. Strain Ma-21(T) was found to grow optimally at 37 °C and pH 8.0 with 3% (w/v) NaCl.