Near-Infrared Light Driven Reversible Photoelectrochemical Bioassay by S-Scheme All-Polymer Blends for Acetylcholinesterase Activity Monitoring.

Photoelectrochemical (PEC) biosensing, recognized for its heightened sensitivity, faces limitations in its application for diagnosis due to the inefficiency of UV-visible light-driven photoactive materials in nontransparent biological samples. In this study, we investigate the potential of an S-scheme all-polymer heterojunction comprising a prototype nonfullerene polymeric acceptor (PYIT) and carbon nitride to develop a near-infrared (NIR) light-driven PEC biosensor for monitoring acetylcholinesterase activity in nontransparent human whole blood. The distinct molecular structure of PYIT enables efficient light absorption in the NIR region, enhancing sensitivity in nontransparent biological samples.

Applications of DNA functionalized gold nanozymes in biosensing.

In recent years, nanozymes have emerged as highly potential substitutes, surpassing the performance of natural enzymes. Among them, gold nanoparticles (AuNPs) and their metal hybrids have become a hot topic in nanozyme research due to their facile synthesis, easy surface modification, high stability, and excellent enzymatic activity. The integration of DNA with AuNPs, by precisely controlling the assembly, arrangement, and functionalization of nanoparticles, greatly facilitates the development of highly sensitive and selective biosensors.

The Impact of Nutrient Solution Electrical Conductivity on Leaf Transcriptome Contributing to the Fruit Quality of Cucumber Grown in Coir Cultivation.

Soilless cultivation is increasingly utilized in supplying essential nutrients for greenhouse crops. However, the impact of coir cultivation under varying electrical conductivity (EC) conditions on cucumber growth and fruit quality, particularly through the regulation of gene expression during the vegetative stage, remains uncertain. In this study, we performed metabolic measurements on cucumber in both vegetative and reproductive stages under three different EC conditions and found metabolic products such as some primary metabolites (cellulose, many uncharged amino acids) and some secondary metabolites (rutin, cucurbitacin B) accumulated the most under EC of 5 dS·m.

A soluble garlic polysaccharide supplement alleviates fatigue in mice.

Garlic polysaccharide (GP) is an inulin-type fructan with potent antioxidant activity, whereas its health-promoting functions are not well explored. In the current study, we established a fatigue model by forcing mice to swim in a water tank for at least one hour every day. We measured changes in body weight, exhaustive swimming time, and biochemical indices related to fatigue in weight-bearing swimming mice fed low (1.

Two-Dimensional Electrically Conductive Metal-Organic Framework Boosts Synaptic Plasticity for Dynamic Image Refresh, Classification, and Efferent Neuromuscular Systems.

We present a two-dimensional (2D) electrically conductive metal-organic framework (EC-MOF)-based artificial synapse. The intrinsic electronic conductivity and subnanometer channels of the EC-MOF facilitate efficient ion diffusion, enable a high density of active redox centers, and significantly enhance capacitance within the artificial synapse. As a result, the synapse operates at an ultralow voltage of 10 mV and exhibits a remarkably low power consumption of approximately 1 fW, along with the longest retention time recorded for two-terminal electrolyte-type artificial synapses to date.

Deep learning based ultra-low dose fan-beam computed tomography image enhancement algorithm: Feasibility study in image quality for radiotherapy.

Objective: We investigated the feasibility of deep learning-based ultra-low dose kV-fan-beam computed tomography (kV-FBCT) image enhancement algorithm for clinical application in abdominal and pelvic tumor radiotherapy.

Methods: A total of 76 patients of abdominal and pelvic tumors were prospectively selected. The Catphan504 was acquired with the same conditions as the standard phantom test set.

Timing of endoscopy in cirrhotic patients with acute variceal bleeding: protocol of a multicenter randomized controlled trial.

Background: Endoscopy is important for the diagnosis and treatment of acute upper gastrointestinal bleeding (AUGIB), especially acute variceal bleeding (AVB), in liver cirrhosis. However, the optimal timing of endoscopy remains controversial, primarily because the currently available evidence is of poor quality, and the definition of early endoscopy is also very heterogeneous among studies. Herein, a multicenter randomized controlled trial (RCT) is performed to explore the impact of the timing of endoscopy on the outcomes of cirrhotic patients with AVB.

Electroacupuncture extends the time window of thrombolytic therapy in rats by reducing disruptions of blood-brain barrier and inhibiting GSDMD-mediated pyroptosis.

Objective: Thrombolytic therapy is the primary treatment for acute ischemic stroke. Extending the therapeutic time window can effectively reduce the harmful side effects associated with thrombolytic therapy. Although electroacupuncture (EA) has been shown to extend this time window, the specific mechanisms remain unclear.

Ultrasensitive Flexible Organic Synaptic Transistors Modulated by a Chemically Cross-Linked Solvent-Resistive Ion Composite.

We demonstrate a flexible organic synaptic transistor (FOST) with an ion-composite electrolyte film resistant to chemical reagents, which uses a three-dimensionally cross-linked polyimide matrix to accommodate a high-concentration ionic liquid. FOST shows versatile synaptic plasticity for classical conditioning, high-pass filtering, and the learning-forgetting process. The device achieves low-energy consumption down to 1.

Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor.

Engineered transcription factors (eTFs) binding diversed functional nucleic acids (dFNAs), as innovative biorecognition systems, have gradually become indispensable core elements for building synthetic biosensors. They not only circumvent the limitations of the original TF-based biosensing technologies, but also inject new vitality into the field of synthetic biosensing. This review aims to provide the first comprehensive and systematic dissection of the eTF-dFNA synthetic biosensor concept.

A gold nanoparticle-enhanced dCas9-mediated fluorescence resonance energy transfer for nucleic acid detection.

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas proteins coupled with pre-amplification have shown great potential in molecular diagnoses. However, the current CRISPR-based methods require additional reporters and time-consuming process. Herein, a gold nanoparticle (AuNP)-enhanced CRISPR/dCas9-mediated fluorescence resonance energy transfer (FRET) termed Au-CFRET platform was proposed for rapid, sensitive, and specific detection of nucleic acid for the first time.

Innovative In Situ Passivation Strategy for High-Efficiency Sb(S,Se) Solar Cells.

An effective defect passivation strategy is crucial for enhancing the performance of antimony selenosulfide (Sb(S,Se)) solar cells, as it significantly influences charge transport and extraction efficiency. Herein, a convenient and novel in situ passivation (ISP) technique is successfully introduced to enhance the performance of Sb(S,Se) solar cells, achieving a champion efficiency of 10.81%, which is among the highest recorded for Sb(S,Se) solar cells to date.

WormCNN-Assisted Establishment and Analysis of Glycation Stress Models in : Insights into Disease and Healthy Aging.

Glycation Stress (GS), induced by advanced glycation end-products (AGEs), significantly impacts aging processes. This study introduces a new model of GS of by feeding them OP50 cultured in a glucose-enriched medium, which better simulates human dietary glycation compared to previous single protein-glucose cross-linking methods. Utilizing WormCNN, a deep learning model, we assessed the health status and calculated the Healthy Aging Index (HAI) of worms with or without GS.

A mucoadhesive-to-penetrating nanomotors-in-hydrogel system for urothelium-oriented intravesical drug delivery.

Intravesical therapy (IT) is widely used to tackle various urological diseases. However, its clinical efficacy is decreased by the impermeability of various barriers presented on the bladder luminal surface, including the urinary mucus layer and the densely packed tissue barrier. In this study, we report a mucoadhesive-to-penetrating nanomotors-in-hydrogel system for urothelium-oriented intravesical drug delivery.

A Gene Cluster of Mitochondrial Complexes Contributes to the Cognitive Decline of COVID-19 Infection.

"Brain fog," a persistent cognitive impairment syndrome, stands out as a significant neurological aftermath of coronavirus disease 2019 (COVID-19). Yet, the underlying mechanisms by which COVID-19 induces cognitive deficits remain elusive. In our study, we observed an upregulation in the expression of genes linked to the inflammatory response and oxidative stress, whereas genes associated with cognitive function were downregulated in the brains of patients infected with COVID-19.

Biotemplated Janus Magnetic Microrobots Based on Diatomite for Highly Efficient Detection of Salmonella.

Foodborne illnesses caused by Salmonella bacteria pose a significant threat to public health. It is still challenging to detect them effectively. Herein, biotemplated Janus disk-shaped magnetic microrobots (BJDMs) based on diatomite are developed for the highly efficient detection of Salmonella in milk.

Synaptic Transistors Using Scalable Graphene Nanoribbons.

Graphene has demonstrated potential for use in neuromorphic electronics due to its superior electrical properties. However, these devices are all based on graphene sheets without patterning, restricting its applications. Here, we demonstrate a graphene nanoribbon synaptic transistor (GNST), with the graphene nanoribbon (GNR) channels fabricated using an electro-hydrodynamically printed nanowire array as lithographic masks for scalable fabrication.

Novel catalytic behavior of defective nanozymes with catalase-mimicking characteristics for the degradation of tetracycline.

Although nanozymes have shown significant potential in wastewater treatment, enhancing their degradation performance remains challenging. Herein, a novel catalytic behavior was revealed for defective nanozymes with catalase-mimicking characteristics that efficiently degraded tetracycline (TC) in wastewater. Hydroxyl groups adsorbed on defect sites facilitated the in-situ formation of vacancies during catalysis, thereby replenishing active sites.