Coupling CRISPR-Cas and a personal glucose meter with an enzymatic reporter for portable detection of human papillomavirus in biological samples.

Significant efforts and resources have been dedicated to developing CRISPR-Cas based point-of-care testing (POCT) and self-diagnosis methods for nucleic acid pathogens, aiming to complement the gold standard quantitative PCR tests, particularly in settings where centralized facilities, trained personnel, or resource-intensive equipment are unavailable. However, the reliance on stationary, high-cost readout machinery hinders their full deployment at the point of care. We aimed to develop a solid-phase invertase-labeled reporter (ILR) system that enables convenient readout of CRISPR-Cas reactions, facilitate HPV detection in a POCT-compatible manner.

Exploring the Core Functional Microbiota Related to Flavor Compounds in Douchi from the Sichuan-Chongqing Region.

Douchi is a traditional Chinese fermented soybean product. In the Sichuan-Chongqing region, -type douchi was particularly famous for its distinctive flavor. Nevertheless, the association between microorganisms and douchi flavor is still poorly understood.

Enhanced Luminescence and Thermal Stability in High Gd/Eu Co-Doped BaYO Phosphors via Co-Precipitation Method.

The co-precipitation method was successfully used to synthesize Ba(YGdEu)O (0.01 ≤ x ≤ 0.09) phosphors with heavy Gd doping, resulting in significantly enhanced thermal stability and luminescence performance.

The sex-specific difference in age-related aortic regional morphological changes.

Background: This study aims to investigate the influence of sex on age-related changes in aortic morphology using computed tomography (CT) imaging.

Method: Patients who underwent contrast-enhanced chest and abdominal CT between July 2021 and April 2022 were enrolled and stratified into six groups. Sex-specific comparisons of body surface area (BSA)-adjusted aortic diameters and tortuosity were performed across different groups.

Gold-Catalyzed 1,2-Rearrangement on Benzene Triggered by a Transient Dearomative Claisen Rearrangement.

1,2-Migration on arenes represents a powerful transformation, because of its chemical skeleton and exit vector editing ability. However, the corresponding dearomative mediated 1,2-migration on arene was ignored for decades, despite tremendous effort being devoted to develop the dearomative reaction, which mainly capitalized on breaking the planarity for three-dimensional diversification. Here, we report on the gold-catalyzed 1,2-rearrangement on benzene by the transient dearomatization of high-resonance energy benzene.

Lacidophilin tablets relieve irritable bowel syndrome in rats by regulating gut microbiota dysbiosis and intestinal inflammation.

Irritable bowel syndrome (IBS) is a common clinical functional gastrointestinal disease. It has a complex pathophysiological mechanism, in which the imbalance of gut microbiota might play an important role. Lacidophilin tablets (LH) can regulate gut microbiota, but their effect on IBS is unknown.

Comparative studies on microbial transglutaminase, complex phosphate and fructooligosaccharide interacts with myofibrillar proteins: Improvement of the quality and flavor of silver carp surimi.

The aim of this study was to evaluate the effect of microbial transglutaminase (MTG), complex phosphate (CP) and fructooligosaccharide (FOS) interact with myofibrillar proteins (MPs) on the quality and flavor of silver carp surimi gels. When surimi was added with 0.3 % MTG, 0.

Avian tuberculosis identified as the potential disease in an outbreak in wild migratory birds in China.

This study identifies avian tuberculosis as a potential cause of mass mortality in wild migratory birds in Inner Mongolia, China. Combining meta-transcriptomic sequencing and histopathological analysis, it reveals one of the rare instances of tuberculosis-associated outbreaks in avian populations. These findings underscore the importance of surveillance on wildlife diseases to mitigate the risk of interspecies transmission of the disease associated pathogens and their broader implications for biodiversity and public health.

Recent Progress in Radiosensitive Nanomaterials for Radiotherapy-Triggered Drug Release.

Benefiting from the unique properties of ionizing radiation, such as high tissue penetration, spatiotemporal resolution, and clinical relevance compared with other external stimuli, radiotherapy-induced drug release strategies are showing great promise in developing effective and personalized cancer treatments. However, the requirement of high doses of X-ray irradiation to break chemical bonds for drug release limits the application of radiotherapy-induced prodrug activation in clinics. Recent advances in nanomaterials offer a promising approach for radiotherapy sensitization as well as integrating multiple modalities for improved therapy outcomes.

Bone marrow immune cells and drug resistance in acute myeloid leukemia.

In recent years, the relationship between the immunosuppressive niche of the bone marrow and therapy resistance in acute myeloid leukemia (AML) has become a research focus. The abnormal number and function of immunosuppressive cells, including regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSCs), along with the dysfunction and exhaustion of immunological effector cells, including cytotoxic T lymphocytes (CTLs), dendritic cells (DCs) and natural killer cells (NKs), can induce immune escape of leukemia cells and are closely linked to therapy resistance in leukemia. This article reviews the research progress on the relationship between immune cells in the marrow microenvironment and chemoresistance in AML, aiming to provide new ideas for the immunotherapy of AML.

Quality of guidelines for infection management in sepsis: a critical appraisal using the AGREE II instrument.

Objectives: The aim of this study was to systematically assess the methodological quality of current sepsis infection management guidelines and identify gaps in knowledge that limit evidence-based practice.

Methods: A systematic search was conducted to obtain guidelines for the management of sepsis infections (2012-2021), and three reviewers independently assessed the quality of eligible guidelines using Appraisal of Guidelines for Research and Evaluation (AGREE II) tool. The intraclass correlation coefficients (ICCs) were used to measure the agreement between reviewers.

Multilevel, solar-blind, and thermostable physical unclonable functions based on host-sensitized luminescence of β-GaO:Dy .

Optical physical unclonable functions (PUFs) are powerful tools to combat counterfeiting, owing to their unpredictable preparation processes and unique, identifiable information content. Achieving high entropy and robustness in optical PUFs is essential for practical applications but remains challenging. This study demonstrates a multilevel, solar-blind, and thermostable PUF based on host-sensitized luminescence of trivalent dysprosium (Dy) in β-phase gallium oxide (β-GaO).

Mobility reversal and absolute negative mobility induced by an internal degree of freedom.

We study a Brownian motor that consists of two linearly coupled particles moving in a symmetric periodic potential and subjected to an unbiased harmonic driving in combination with a constant external force. Although a trajectory of the single-particle system must also be a valid trajectory of the corresponding coupled-particle system, the stability of the trajectory does not necessarily hold. It is revealed that, for proper strength of the interparticle coupling K, parametric resonance, which may break the stability of the trajectory, emerges.

WRKY transcription factor MdWRKY71 regulates flowering time in apple.

In plants, flowering is crucial to reproductive success. Receiving limited attention in apple research is the function of WRKY transcription factors in regulating flowering time. We characterized a WRKY transcription factor, MdWRKY71, from red-fleshed apple in this study, and ectopically expressed it in Arabidopsis thaliana, which revealed its role in flowering.

Coupling ZnN Atomic Sites with Graphitic Nitrogen for Enhanced Ammonium Production via Electrocatalytic Nitrate Reduction.

The electrocatalytic nitrate reduction reaction (NORR) presents a promising alternative to the traditional Haber-Bosch process for synthesizing ammonium (NH) under mild conditions. Supported single atoms have emerged as active catalysts for this conversion; however, the efficiency of NH production are limited by the modest activity and selectivity of these catalysts. In this study, it is demonstrated that zinc single atoms supported on nitrogen-doped carbon (ZnNCs), derived from zeolitic imidazolate framework-8 (ZIF-8), possess remarkable catalytic performance for this conversion, achieving an unprecedented selectivity (exceeding 90%) for NH formation across a broad potential range (-0.

Screening potential antileukemia ingredients from sweet potato: integration of metabolomics analysis, network pharmacology, and experimental validation.

Background: Active dietary flavonoids are a promising resource for novel drug discovery. Sweet potato, a widely cultivated functional crop, is abundant in flavonoids. However, the active ingredients associated with acute myeloid leukemia (AML) treatment and their underlying mechanisms have not been reported to date.

Development of a dual-responsive injectable GelMA/F127DA hydrogel for enhanced cartilage regeneration in osteoarthritis: Harnessing MMP-triggered and mechanical stress-induced release of therapeutic agents.

Osteoarthritis (OA) presents a significant challenge in clinical settings due to the limited self-renewal capability of cartilage tissue. To address this, engineered biomaterials employing biomimetic strategies have been developed to modulate and enhance cell-microenvironment interactions, facilitating cartilage regeneration. Nonetheless, excessive mechanical stress on joint structures can induce inflammatory responses, thereby impeding the process of cartilage repair.

Implementation of multiple soliton state fiber laser using a promising saturable absorber of silver indium phosphorus sulfide.

Based on the nonlinear broadband saturation absorption, ultrafast carrier recovery rate, and ultrashort recovery time of new low dimensional materials, ultrafast photonics has rapidly developed as an interdisciplinary field. We have demonstrated multiple solitons state output in an erbium-doped fiber laser based on saturable absorption characteristic of AgInPS. By preparing AgInPS with a modulation depth of 10.

Investigations of modulation method and stress mechanism for the growth of AlGaN channel heterostructures.

In this work, the strong connection between the channel and the barrier layer of AlGaN channel heterostructures has been investigated in detail. Unlike GaN as a channel material, AlGaN channel layers significantly influence the transport characteristics and quality of AlGaN barrier layers with increasing Al composition. Furthermore, the stress mechanism in the growth of the AlGaN layer has been thoroughly discussed.

Comparative proteomic landscapes elucidate human preimplantation development and failure.

Understanding mammalian preimplantation development, particularly in humans, at the proteomic level remains limited. Here, we applied our comprehensive solution of ultrasensitive proteomic technology to measure the proteomic profiles of oocytes and early embryos and identified nearly 8,000 proteins in humans and over 6,300 proteins in mice. We observed distinct proteomic dynamics before and around zygotic genome activation (ZGA) between the two species.

The regulatory module MdCPCL-MdILR3L mediates the synthesis of ascorbic acid and anthocyanin in apple.

Apple (Malus domestica Borkh.) is one of the most economically valuable fruit crops globally and a key dietary source for various nutrients. However, the levels of ascorbic acid (AsA) and anthocyanin, essential micronutrients for human health, are extremely low in the pulp of commonly cultivated apple varieties.

Deep Learning for Discrimination of Early Spinal Tuberculosis from Acute Osteoporotic Vertebral Fracture on CT.

Background: Early differentiation between spinal tuberculosis (STB) and acute osteoporotic vertebral compression fracture (OVCF) is crucial for determining the appropriate clinical management and treatment pathway, thereby significantly impacting patient outcomes.

Objective: To evaluate the efficacy of deep learning (DL) models using reconstructed sagittal CT images in the differentiation of early STB from acute OVCF, with the aim of enhancing diagnostic precision, reducing reliance on MRI and biopsies, and minimizing the risks of misdiagnosis.

Methods: Data were collected from 373 patients, with 302 patients recruited from a university-affiliated hospital serving as the training and internal validation sets, and an additional 71 patients from another university-affiliated hospital serving as the external validation set.

Structure Elucidation and Immunostimulatory Activity Evaluation of a Galactoglucan from Hance.

Hance has a medicinal history of thousands of years in treating cough, diabetes, and gastrointestinal system diseases, and it is also a medicine food homology (MFH) plant in China. To evaluate the pharmacological activities of polysaccharides from the rhizomes of , polysaccharides were initially obtained by hot-water extraction and the ethanol precipitation method. A homogenous polysaccharide designated as AOP-w was isolated by a DE-52 column.

Genomic characteristics and phylogenetic relationships of Cutibacterium acnes breast milk isolates.

Background: Cutibacterium acnes is one of the most commonly found microbes in breast milk. However, little is known about the genomic characteristics of C. acnes isolated from breast milk.