In silico characterization of defense system hotspots in Acinetobacter spp.

The bacteria-phage arm race drives the evolution of diverse bacterial defenses. This study identifies and characterizes the defense hotspots in Acinetobacter baumannii using a reference-free approach. Among 4383 high-quality genomes, we found a total of 17,430 phage defense systems and with 54.

Precision DNA methylation typing via hierarchical clustering of Nanopore current signals and attention-based neural network.

Decoding DNA methylation sites through nanopore sequencing has emerged as a cutting-edge technology in the field of DNA methylation research, as it enables direct sequencing of native DNA molecules without the need for prior enzymatic or chemical treatments. During nanopore sequencing, methylation modifications on DNA bases cause changes in electrical current intensity. Therefore, constructing deep neural network models to decode the electrical signals of nanopore sequencing has become a crucial step in methylation site identification.

Bridged triphenylamine-based fluorescent probe for selective and direct detection of HSA in urine.

Human serum albumin (HSA) serves as a crucial indicator for therapeutic monitoring and biomedical diagnosis. In this study, a near infrared (NIR) fluorescent probe, termed BTPA, characterized a donor-π-acceptor (D-π-A) structure based on bridged triphenylamine (TPA) was developed. BTPA exhibited outstanding sensitivity and selectivity towards HSA among various analysts, with a remarkable 50-fold fluorescence enhancement with a significant Stokes shift (∼190 nm) and a wide linear detection range of 0-20 μM of HSA.

Global remote feature modulation end-to-end detection.

Object detector based on fully convolutional network achieves excellent performance. However, existing detection algorithms still face challenges such as low detection accuracy in dense scenes and issues with occlusion of dense targets. To address these two challenges, we propose an Global Remote Feature Modulation End-to-End (GRFME2E) detection algorithm.

Effect of Calcination Atmosphere on the Performance of Cu/AlO Catalyst for the Selective Hydrogenation of Furfural to Furfuryl Alcohol.

The selective hydrogenation of the biomass platform molecule furfural (FAL) to produce furfuryl alcohol (FA) is of great significance to alleviate the energy crisis. Cu-based catalysts are the most commonly used catalysts, and their catalytic performance can be optimized by changing the preparation method. This paper emphasized the effect of calcination atmosphere on the performance of a Cu/AlO catalyst for the selective hydrogenation of FAL.

Huanglian Decoction treats Henoch-Schonlein purpura nephritis by inhibiting NF-κB/NLRP3 signaling pathway and reducing renal IgA deposition.

Henoch-Schonlein purpura nephritis (HSPN) is a systemic vascular inflammatory disease. Huanglian Decoction (HLD) ameliorates renal injury in nephritis; however, the mechanism of action of HLD on HSPN has not been investigated. This study aimed to investigate the protective mechanism of HLD treatment in HSPN.

Visible-Light-Driven Inactivation of Bacteria and H Generation Catalyzed by Oxygen-Vacancy-Rich One-Dimensional/Two-Dimensional WO/g-CN Z-Scheme Heterostructures.

Z-scheme heterostructure-based photocatalysts consist of a reduction photocatalyst and an oxidation photocatalyst, enabling them to possess a high capacity for both reduction and oxidation. However, the coupling reaction between photocatalytic H generation through water reduction and sterilization using Z-scheme systems has been rarely reported. Herein, 1D WO nanowires embedded over 2D g-CN nanosheets are well-constructed as an integrated Z-scheme heterojunction.

[Effects of Formula Combined with Prednisone on Immune Function and ST2/IL-33 Pathway in Mice with Immune Thrombocytopenia].

Objective: To investigate the effects of formula combined with prednisone on immune function and the ST2/IL-33 pathway in mice with immune thrombocytopenia.

Methods: In 40 BALB/c mice, 32 were constructed as immune thrombocytopenia mouse models by antiplatelet serum injection. After successful modeling, the mice were randomly divided into model group, formula group (0.

Parasitic fish embryos do a "front-flip" on the yolk to resist expulsion from the host.

Embryonic development is often considered shielded from the effects of natural selection, being selected primarily for reliable development. However, embryos sometimes represent virulent parasites, triggering a coevolutionary "arms race" with their host. We have examined embryonic adaptations to a parasitic lifestyle in the bitterling fish.

Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway.

Context: Chronic myeloid leukemia (CML) is a malignant hematopoietic stem cell disease caused by excessive proliferation and abnormal differentiation of hematopoietic stem cells. (ASP) is considered to have good biological activity and may be a good anti-CML drug.

Objective: This study aimed to explore the effects and possible mechanisms of ASP on the biological behavior of K562 cells based on RNA-seq.

Medial Septal Glutamatergic Neurons Modulate States of Consciousness during Sevoflurane Anesthesia in Mice.

Background: Multiple neural structures involved in maintaining wakefulness have been found to promote arousal from general anesthesia. The medial septum is a critical region that modulates arousal behavior. This study hypothesized that glutamatergic neurons in the medial septum play a crucial role in regulating states of consciousness during sevoflurane general anesthesia.

Clinical research on the relationship between the curve of Wilson and temporomandibular joint disorders.

Objective: The aim of this study was to examine the relationship between the curve of Wilson (COW) and temporomandibular joint disorder (TMD).

Methods: The study cohort comprised patients aged 19-55 with malocclusion treated at our institution from January to July 2021. They were divided into a malocclusion with TMD group (TMD group) and a malocclusion without TMD group (non-TMB group) based on the diagnostic criteria of TMD.

[Effect of P-coumaric Acid on Apoptosis of Multiple Myeloma Cells Based on Oxidative Stress].

Objective: To investigate the effect of p-coumaric acid on apoptosis of multiple myeloma cells and its related mechanism.

Methods: Multiple myeloma cell line MM.1s cells were selected and treated with different concentrations of p-coumaric acid (0, 0.

GSH-Activatable Aggregation-Induced Emission Cationic Lipid for Efficient Gene Delivery.

The key to gene therapy is the design of biocompatible and efficient delivery systems. In this work, a glutathione (GSH)-activated aggregation-induced-emission (AIE) cationic amphiphilic lipid, termed QM-SS-KK, was prepared for nonviral gene delivery. QM-SS-KK was composed of a hydrophilic biocompatible lysine tripeptide headgroup, a GSH-triggered disulfide linkage, and a hydrophobic AIE fluorophore QM-OH (QM: quinoline-malononitrile) tail.

Al-Incorporated Cobalt-Layered Double Hydroxides for Enhanced Oxygen Evolution through Morphology and Electronic Structure Regulation.

Layered double hydroxides (LDHs) are promising electrocatalytic materials for the oxygen evolution reaction (OER) due to their tunable composition and low cost. Here, we construct ultrathin Al-incorporated Co LDH nanosheets on carbon cloth (CC) by a facile hydrothermal strategy. Compared to Co LDH/CC, the optimized CoAl LDH/CC displays significantly improved OER performance, characterized by low overpotentials of only 171 and 200 mV to reach current densities of 10 mA cm in alkaline and neutral media, respectively, as well as good stability over an extended period.

Comparative Study on Feature Selection in Protein Structure and Function Prediction.

Many effective methods extract and fuse different protein features to study the relationship between protein sequence, structure, and function, but different methods have preferences in solving the research of protein structure and function, which requires selecting valuable and contributing features to design more effective prediction methods. This work mainly focused on the feature selection methods in the study of protein structure and function, and systematically compared and analyzed the efficiency of different feature selection methods in the prediction of protein structures, protein disorders, protein molecular chaperones, and protein solubility. The results show that the feature selection method based on nonlinear SVM performs best in protein structure prediction, protein solubility prediction, protein molecular chaperone prediction, and protein solubility prediction.

Identification of Drug-Disease Associations Using a Random Walk with Restart Method and Supervised Learning.

Drug-disease correlations play an important role in revealing the mechanism of disease, finding new indications of available drugs, or drug repositioning. A variety of computational approaches were proposed to find drug-disease correlations and achieve good performances. However, these methods used a variety of network information, but integrated networks were rarely used.

Developmental neuroanatomy of the rosy bitterling Rhodeus ocellatus (Teleostei: Cypriniformes)-A microCT study.

Bitterlings are carp-like teleost fish (Cypriniformes: Acheilanathidae) known for their specialized brood parasitic lifestyle. Bitterling embryos, in fact, develop inside the gill chamber of their freshwater mussel hosts. However, little is known about how their parasitic lifestyle affects brain development in comparison to nonparasitic species.

HPVMD-C: a disease-based mutation database of human papillomavirus in China.

Human papillomavirus (HPV) can cause condyloma acuminatum and cervical cancer. Some mutations of these viruses are closely related to the persistent infection of cervical cancer and are ideal cancer vaccine targets. Several databases have been developed to collect HPV sequences, but no HPV mutation database has been published.

The effect of two different doses of dexmedetomidine to prevent emergence agitation in children undergoing adenotonsillectomy: a randomized controlled trial.

Objective: To evaluate different doses of dexmedetomidine for the prevention of emergence agitation in children undergoing adenotonsillectomy.

Method: One hundred and thirty children aged 3-10 years scheduled for adenotonsillectomy were randomly assigned to two groups. Anesthesia was induced with 0.

Screening and identification of potential novel lipid biomarkers for non-small cell lung cancer using ultra-high performance liquid chromatography tandem mass spectrometry.

Lung cancer is characterized by a high incidence rate and low survival rate. It is important to achieve early diagnosis of the disease. We applied ultra-high performance liquid chromatography tandem mass spectrometry to screen plasma lipid spectrum in non-small cell lung cancer (NSCLC) patients, healthy controls (HC), and community-acquired pneumonia (CAP) patients.

Normal stages of embryonic development of a brood parasite, the rosy bitterling Rhodeus ocellatus (Teleostei: Cypriniformes).

Bitterlings, a group of freshwater teleosts, provide a fascinating example among vertebrates of the evolution of brood parasitism. Their eggs are laid inside the gill chamber of their freshwater mussel hosts where they develop as brood parasites. Studies of the embryonic development of bitterlings are crucial in deciphering the evolution of their distinct early life-history.

Metal-free g-CN nanosheets as a highly visible-light-active photocatalyst for thiol-ene reactions.

Thiol-ene click reactions are important for the construction of carbon-sulfur bonds. The use of visible-light photoredox catalysis for the formation of C-S bonds has attracted much attention. In this work, two-dimensional metal-free graphitic carbon nitride (g-CN) nanosheets are prepared through a simple thermal polymerization method and used to catalyze the thiol-ene click reaction under visible light-illumination.

Novel therapeutic evaluation biomarkers of lipid metabolism targets in uncomplicated pulmonary tuberculosis patients.

Currently, the management of pulmonary tuberculosis (TB) lacks potent medications and accurate efficacy evaluation biomarkers. In view of the fact that the host lipids are the important energy source of Mycobacterium tuberculosis (Mtb), UPLC-MS/MS based on lipid metabolism was used to monitor the plasma lipid spectrum of TB patients from the initial diagnosis to cured. The analysis showed that TB patients presented aberrant metabolism of phospholipids, glycerides, and sphingolipids.