Publications by authors named "WenHong Fan"

In biological systems, nanobubbles (NBs) effectively enhance hydrogen molecule retention and scavenging reactive oxygen species (ROS), but the underlying mechanisms remain elusive. To investigate this, we prepared hydrogen NB water samples with consistent dissolved hydrogen levels but varying NB densities to explore their physicochemical properties and effects on green algae (Chlorella vulgaris) under oxidative stress induced by copper ions (Cu) and cadmium ions (Cd). The results indicated a strong correlation between the hydrogen NB number density and the 25 % inhibitory concentration of Cu over 24 h, with ROS removal efficiency increased with the NB number density.

View Article and Find Full Text PDF

With the rapid increase in the number of commercial chemicals, testing methods regarding on median lethal dose (LD) relying animal experiments face challenges such as high costs and ethical concerns. Classical quantitative structure-activity relationship models relying on single algorithm always lack interpretability and precision, given the complexity of the mechanisms underlying acute toxicity. To address these issues, this study has developed a predictive framework using an ensemble learning model based on Super-learner.

View Article and Find Full Text PDF

The massive production and application of nanomaterials (NMs) have raised concerns about the potential adverse effects of NMs on human health and the environment. Evaluating the adverse effects of NMs by laboratory methods is expensive, time-consuming, and often fails to keep pace with the invention of new materials. Therefore, methods that utilize machine learning techniques to predict the toxicity potentials of NMs are a promising alternative approach if regulatory confidence in them can be enhanced.

View Article and Find Full Text PDF

Widely-used C fullerene nanoparticles (C) result in their release into the aquatic environment, which may affect the distribution and toxicity of pollutants such as arsenic (As), to aquatic organism. In this study, arsenate (As(V)) accumulation, speciation and subcellular distribution was determined in Danio rerio (zebrafish) intestine, head and muscle tissues in the presence of C. Meanwhile we compared how single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO) and graphene (GN) nanoparticles alter the behaviors of As(V).

View Article and Find Full Text PDF

The hydrogen molecule can effectively regulate plant growth and development, improving plant resistance to abiotic stresses. However, studies regarding the optimal concentration of hydrogen and the associated mechanisms of action in organisms are lacking. This study showed that the maximum germination rate of radish seeds decreased from 90 % to 50 % under the stress of cadmium ions (Cd), and hydrogen nanobubble (NB) water significantly alleviated the stress effect of Cd on radish seed germination.

View Article and Find Full Text PDF

Sialylation is an important modification of proteins, related to protein life and bioactivity. However, the evaluation of sialylation is only based on the average molecular composition by peptide mapping and glycan profiling because sialylated proteins are usually too heterogeneous to obtain good quality mass spectra by conventional intact mass analysis methods. In this study, a simple strong cation exchange-mass spectroscopy (SCX-MS) method was developed for intact mass analysis of sialylated glycoproteins.

View Article and Find Full Text PDF

Currently, adeno-associated virus (AAV) is one of the primary gene delivery vectors in gene therapy, facilitating long-term gene expression. Despite being imperative, it is incredibly challenging to precisely assess AAV particle distribution according to the sedimentation coefficient and identify impurities related to capsid structures. This study performed the systematic methodological validation of quantifying the AAV empty and full capsid ratio.

View Article and Find Full Text PDF

Recombinant proteins are gaining increasing popularity for treating human diseases. The clinical effectiveness of recombinant proteins is directly related to their biological activity, which is an important indicator in drug development and quality control. However, certain recombinant proteins have unclear or complex signal pathways, making detecting their activity in vitro difficult.

View Article and Find Full Text PDF

Cosmetics and personal care products containing titanium dioxide nanoparticles (TiO NPs) may enter aquatic environments, where the surface coatings of TiO NPs may change with aging due to environmental factors such as light, and potentially affect their bioaccumulation and toxicity. This study examined how aging impacted the physicochemical properties of three commercially available TiO NPs and subsequent influence on the bioaccumulation and toxicity of copper (Cu) in Daphnia magna (D. magna).

View Article and Find Full Text PDF

Dissolved organic matter (DOM) in wastewater interacts with heavy metal particles in aquatic environments, which changes their dynamics and bioavailability. For quantifying the DOM, an excitation-emission matrix (EEM) paired alongside parallel factor analysis (PARAFAC) is typically employed. However, a drawback of PARAFAC has been revealed in recent studies, i.

View Article and Find Full Text PDF

In clinical practice, radiopharmaceutical dynamic imaging technology requires the bolus injection method to complete injection. Due to the failure rate and radiation damage of manual injection, even experienced technicians still bear a lot of psychological burden. This study combined the advantages and disadvantages of various manual injection modes to develop the radiopharmaceutical bolus injector, and explored the application of automatic injection in the field of bolus injection from four aspects: radiation protection, occlusion response, sterility of injection process and effect of bolus injection.

View Article and Find Full Text PDF

Rationale: Fc-fusion proteins represent a successful class of biopharmaceutical products, which combine the tailored pharmacological properties of biological ligands with the multiple functions of the fragment crystallizable domain of immunoglobulins. There is great diversity in terms of possible biological ligands creating highly diverse structures, therefore the analytical characterization of fusion proteins is far more complex than that of monoclonal antibodies and requires the use and development of additional product-specific methods over conventional generic/platform methods.

Methods: Employing etanercept analogues as studied fusion proteins, the Orbitrap mass analyzer with ultra-high performance liquid chromatography (UHPLC-MS) and imaged capillary isoelectric focusing (icIEF) were utilized for the in-depth fusion protein characterization.

View Article and Find Full Text PDF

The wide production and use of metallic nanomaterials (MNMs) leads to increased emissions into the aquatic environments and induces high potential risks. Experimentally evaluating the (eco)toxicity of MNMs is time-consuming and expensive due to the multiple environmental factors, the complexity of material properties, and the species diversity. Machine learning (ML) models provide an option to deal with heterogeneous data sets and complex relationships.

View Article and Find Full Text PDF

Transition metal pollution in rivers in South Asia is more serious than in other regions because of the lack of adequate freshwater management measures. Water quality criteria (WQC) for South Asia is urgently needed to protect regional aquatic environments because of the occurrence of transboundary rivers. The present study established non-parametric kernel density estimation species sensitivity distribution (NPKDE-SSD) models and then derived the acceptable hazardous concentration for protection of 95% of all aquatic species (HC5) and WQC of six typical transition metals in South Asia.

View Article and Find Full Text PDF

Due to special properties, nano-TiO will interact with heavy metals and other pollutants in water, thus affecting the environmental behavior and ecotoxicity of these pollutants. However, the exact manner in which nano-TiO affects the bioaccumulation mechanisms of heavy metals is still unclear now. In the present study, quantitative structure bioaccumulation relationship (QSBAR) models were established to explore the relationships between physicochemical parameters of heavy metals and their accumulation in Daphnia magna in the absence and presence of nano-TiO at low metal exposure concentrations.

View Article and Find Full Text PDF

Nanobubbles (NBs) stimulate seed germination; however, the mechanism of the promotion effect of NBs remains unclear. The impact of NBs on seed water absorption was investigated; we subsequently studied the genes associated with the response of radish seeds to NB water and used RNA sequencing to generate their expression profiles, especially those of aquaporin genes. NB water significantly promoted germination.

View Article and Find Full Text PDF

Elevated levels of reactive oxygen radicals caused by environmental stress are the key triggers of inflammation, aging, and disease; thus, it is critical to develop novel reactive oxygen radical scavenging methods with high efficiency and low toxicity. As a result of their selective reactive oxygen radical removal, hydrogen molecules are strong candidates, but their application is limited by the small hydrogen supply and short duration of action. In this study, we for the first time combined nanobubble (NB) technology and hydrogen water to remove reactive oxygen species (ROS) using copper ions as a representative environmental pollutant and as a model organism.

View Article and Find Full Text PDF

The migration of organic matter in salinized lakes was critical in maintaining ecological balance and material circulation process of inland shallow lakes. To clarify the ecological and microbial mechanism of material transport and transformation, the microbial community structure and the characteristics of dissolved organic matter (DOM) in the sediment of Daihai Lake, a typical saline lake at the Yellow River Basin, were explored with three-dimensional excitation and emission matrix fluorescence (3DEEM), parallel factor analysis (PARAFAC) and 16 S rRNA techniques. The correlation between environmental factors, DOM composition and the bacterial community structure were also studied for identifying the key factors of community formation.

View Article and Find Full Text PDF

The behavior and toxicity of nanoparticles could be affected significantly by the ubiquitous natural organic matter (NOM) in aquatic environments. However, the influence of NOM on nanoparticles along the food chain remains largely unknown. This study constructed bacteria Escherichia coli (E.

View Article and Find Full Text PDF

Since December 2019, the 2019 coronavirus disease (COVID-19) outbreak has become a global pandemic. Understanding the role of environmental conditions is important in impeding the spread of COVID-19. Given that airborne spread and contact transmission are considered the main pathways for the spread of COVID-19, this narrative review first summarized the role of temperature and humidity in the airborne trajectory of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

View Article and Find Full Text PDF

The entering of silver nanoparticles (Ag NPs) in natural environments constantly increases due to their widespread production and application. While the environmental behavior, impacts, and fate of Ag NPs were critically assessed, the main challenge represents continuous tracking and quantification of Ag NPs in environmental and biological matrices. A group of labeled Ag NPs with gold cores (Au@Ag NPs) was developed for distinguishing between pristine Ag NPs and their other forms, and we comprehensively compared their physicochemical properties, environmental behavior, and biological effects with unlabeled Ag NPs.

View Article and Find Full Text PDF

Low birthweight (LBW) is a worldwide public health concern, while the global burden of LBW attributable to endocrine-disrupting chemicals, such as per- and polyfluoroalkyl substances (PFAS), has not yet been evaluated. Here, we established a large dataset for the biomonitoring of seven representative congeners of PFAS by examining data from 2325 publications. Global exposure to perfluorooctanesulfonic acid (PFOS) was the highest, followed by perfluorohexanesulfonic acid (PFHxS) and perfluorooctanoic acid (PFOA).

View Article and Find Full Text PDF

In 2020, China for the first time developed guidelines for indoor fine particulate matter (PM) in the draft document of indoor air standards, while the associated health implication remains unclear. Here, we first estimated the PM associated premature deaths was 965 thousand in 2019, with the indoor PM of outdoor origin accounting for 72.9%.

View Article and Find Full Text PDF

Herein, a novel BiOBr photocatalyst with partial surface modification by graphitized C (BiOBr-C) was synthesized through a hydrothermal method with hydrothermal carbonation carbon (HTCC) as a slow-releasing carbon source and characterized by experimental and theoretical methods. BiOBr-C exhibited excellent visible-light photocatalytic performance toward various refractory pollutants, such as bisphenol A, ibuprofen, ciprofloxacin, 2,4-dichlorophenoxyacetic acid, and diphenhydramine. The characterization results demonstrate that a strong molecular orbital interaction occurs between graphitized C and BiOBr, resulting in the formation of a new surface valence band on graphitized C.

View Article and Find Full Text PDF

A novel coronavirus (SARS-CoV-2) causing corona virus disease 2019 (COVID-19) has attracted global attention due to its highly infectious and pathogenic properties. Most of current studies focus on aerosols released from infected individuals, but the presence of SARS-CoV-2 in wastewater also should be examined. In this review, we used bibliometrics to statistically evaluate the importance of water-related issues in the context of COVID-19.

View Article and Find Full Text PDF