Repeat and haplotype aware error correction in nanopore sequencing reads with DeChat.

Error self-correction is crucial for analyzing long-read sequencing data, but existing methods often struggle with noisy data or are tailored to technologies like PacBio HiFi. There is a gap in methods optimized for Nanopore R10 simplex reads, which typically have error rates below 2%. We introduce DeChat, a novel approach designed specifically for these reads.

Negative Emotion Differentiation Promotes Cognitive Reappraisal: Evidence From Electroencephalogram Oscillations and Phase-Amplitude Coupling.

Cognitive reappraisal, an effective emotion regulation strategy, is influenced by various individual factors. Although previous studies have established a link between negative emotion differentiation (NED) and cognitive reappraisal, the underlying neural mechanisms remain largely unknown. Using electroencephalography, this study investigates the influence and neural basis of NED in cognitive reappraisal by integrating aspects of event-related potentials, neural oscillation rhythms, and cross-frequency coupling.

Multifunctional hydrogel based on polyvinyl alcohol/chitosan/metal polyphenols for facilitating acute and infected wound healing.

Bacterial-infected wounds could cause delayed wound healing due to increased inflammation, especially wounds infected by drug-resistant bacteria remain a major clinical problem. However, traditional treatment strategies were gradually losing efficacy, such as the abuse of antibiotics leading to enhanced bacterial resistance. Therefore, there was an urgent need to develop an antibiotic-free multifunctional dressing for bacterially infected wound healing.

HyLight: Strain aware assembly of low coverage metagenomes.

Different strains of identical species can vary substantially in terms of their spectrum of biomedically relevant phenotypes. Reconstructing the genomes of microbial communities at the level of their strains poses significant challenges, because sequencing errors can obscure strain-specific variants. Next-generation sequencing (NGS) reads are too short to resolve complex genomic regions.

Facile Preparation of Multifunctional Hydrogels with Sustained Resveratrol Release Ability for Bone Tissue Regeneration.

Injectable hydrogels show great promise for bone tissue engineering applications due to their high biocompatibility and drug delivery capabilities. The bone defects in osteoporosis are usually characterized by an oxidative and inflammatory microenvironment that impairs the regeneration capability of bone tissues. To attenuate the reactive oxygen species (ROS) and promote bone regeneration, an anti-oxidative hydrogel with osteogenic capacity was developed in this study.

Biomimetic Nanosystem Loading Aggregation-Induced Emission Luminogens and SO Prodrug for Inhibiting Insufficient Photothermal Therapy-Induced Breast Cancer Recurrence and Metastasis.

Photothermal therapy (PTT) holds considerable clinical promise. However, insufficient PTT-induced tumor recurrence and metastasis is an urgent practical problem that needs to be solved. Herein, a biomimetic mesoporous organosilicon nano-system called PSAB is designed to precisely deplete cancer stem cells (CSCs) and prevent tumor recurrence and metastasis after PTT.

AI models predicting breast cancer distant metastasis using LightGBM with clinical blood markers and ultrasound maximum diameter.

Breast cancer metastasis significantly impacts women's health globally. This study aimed to construct predictive models using clinical blood markers and ultrasound data to predict distant metastasis in breast cancer patients, ensuring clinical applicability, cost-effectiveness, relative non-invasiveness, and accessibility of these models. Analysis was conducted on data from 416 patients across two centers, focusing on clinical blood markers (tumor markers, liver and kidney function indicators, blood lipid markers, cardiovascular biomarkers) and maximum lesion diameter from ultrasound.

Development and validation of AI models using LR and LightGBM for predicting distant metastasis in breast cancer: a dual-center study.

Objective: This study aims to develop an artificial intelligence model utilizing clinical blood markers, ultrasound data, and breast biopsy pathological information to predict the distant metastasis in breast cancer patients.

Methods: Data from two medical centers were utilized, Clinical blood markers, ultrasound data, and breast biopsy pathological information were separately extracted and selected. Feature dimensionality reduction was performed using Spearman correlation and LASSO regression.

Fabrication of Coffee-Ring Nanostructured Membranes for Organic Solvent Nanofiltration.

Organic solvent nanofiltration (OSN) plays important roles in pharmaceutical ingredients purification and solvent recovery. However, the low organic solvent permeance under cross-flow operation of OSN membrane hampers their industrial applications. Herein, we report the construction of coffee-ring structured membrane featuring high OSN permeance.

Laponite/lactoferrin hydrogel loaded with eugenol for methicillin-resistant Staphylococcus aureus-infected chronic skin wound healing.

Severe bacterial infections can give rise to protracted wound healing processes, thereby posing a significant risk to a patient's well-being. Consequently, the development of a versatile hydrogel dressing possessing robust bioactivity becomes imperative, as it holds the potential to expedite wound healing and yield enhanced clinical therapeutic outcomes. In this context, the present study involves the formulation of an injectable multifunctional hydrogel utilizing laponite (LAP) and lactoferrin (LF) as foundational components and loaded with eugenol (EG).

The effects of cyberbullying victimization on cyberbullying perpetration among Chinese college students: callous-unemotional traits and the moderating role of Internet morality.

Introduction: The Internet has triggered a series of online deviant behaviors, and cyberbullying is one of them. Cyberbullying victimization as a category of frustration and the aggression triggered by it has been confirmed by many studies. Previous studies have explored the relationship between cyberbullying victimization and cyberbullying perpetration.

Ionic-liquid-modified MOFs incorporated in a mixed-matrix membrane by metal-site anchoring for gas separation.

Through metal-site anchoring, metal-organic frameworks (MOFs) were modified with ionic liquids (ILs) and used as a porous filler to prepare mixed-matrix membranes (MMMs). The targeted growth of the IL exposed more active sites and greatly enhanced CO transfer in the MMMs, which exhibited excellent gas separation performance and long durability.

Tailored Polypyrrole Nanofibers as Ion-to-Electron Transduction Membranes for Wearable K Sensors.

Conductive polymers are recognized as ideal candidates for the development of noninvasive and wearable sensors for real-time monitoring of potassium ions (K) in sweat to ensure the health of life. However, the low ion-to-electron transduction efficiency and limited active surface area hamper the development of high-performance sensors for low-concentration K detection in the sweat. Herein, a wearable K sensor is developed by tailoring the nanostructure of polypyrrole (PPy), serving as an ion-to-electron transduction layer, for accurately and stably tracing the K fluctuation in human sweat.

Lack of Interaction Motivation in Older Adults Automatically Reduces Cognitive Empathy.

Empathy, the ability to understand and respond to the experiences of others, is an important skill for maintaining good relationships throughout one's life. Previous research indicated that emotional empathy remained stable or even increased in older adults compared to younger adults, while cognitive empathy showed age-related deficits. Based on the selective engagement hypothesis, this deficit was not caused by a decline in cognitive functioning, but by a lack of willingness to judge the target person's emotions more precisely, that is, by a lack of interaction motivation.

Postmortem Muscle Proteome Characteristics of Silver Carp (): Insights from Full-Length Transcriptome and Deep 4D Label-Free Proteomic.

The coverage of the protein database directly determines the results of shotgun proteomics. In this study, PacBio single-molecule real-time sequencing technology was performed on postmortem silver carp muscle transcripts. A total of 42.

Membranes based on Covalent Organic Frameworks through Green and Scalable Interfacial Polymerization using Ionic Liquids for Antibiotic Desalination.

Covalent organic framework (COF) membranes featuring uniform topological structures and devisable functions, show huge potential in water purification and molecular separation. Nevertheless, the inability of uniform COF membranes to be produced on an industrial scale and their nonenvironmentally friendly fabrication method are the bottleneck preventing their industrial applications. Herein, we report a new green and industrially adaptable scraping-assisted interfacial polymerization (SAIP) technique to fabricate scalable and uniform TpPa COF membranes.

Gender differences in cognitive and affective interpersonal emotion regulation in couples: an fNIRS hyperscanning.

Emotion regulation is vital in maintaining romantic relationships in couples. Although gender differences exist in cognitive and affective strategies during 'intrapersonal' emotion regulation, it is unclear how gender differences through affective bonds work in 'interpersonal' emotion regulation (IER) in couples. Thirty couple dyads and 30 stranger dyads underwent functional near-infrared spectroscopy hyperscanning recordings when targets complied with their partner's cognitive engagement (CE) and affective engagement (AE) strategies after viewing sad and neutral videos.

Insight into Ionic Strength-Induced Solubilization of Myofibrillar Proteins from Silver Carp (): Structural Changes and 4D Label-Free Proteomics Analysis.

In this study, changes in the physical, structural, and assembly characteristics of silver carp myofibrillar proteins (MPs) at different ionic strength () values were investigated. Moreover, the differential proteomic profile of soluble MPs was analyzed using 4D proteomics based on timsTOF Pro mass spectrometry. Solubility of MPs significantly increased at high (>0.

Automatic Positive and Negative Emotion Regulation in Adolescents with Major Depressive Disorder.

Introduction: Adolescents with major depressive disorder (MDD) exhibit hypoactivity to positive stimuli and hyperactivity to negative stimuli in terms of neural responses. Automatic emotion regulation (AER) activates triple networks (i.e.

Increased Interpersonal Brain Synchronization in Romantic Couples Is Associated with Higher Honesty: An fNIRS Hyperscanning Study.

Previous studies on the brain-brain interaction of deception have shown different patterns of interpersonal brain synchronization (IBS) between different genders. However, the brain-brain mechanisms in the cross-sex composition need to be better understood. Furthermore, there needs to be more discussion about how relationships (e.

Dynamic Alginate Hydrogel as an Antioxidative Bioink for Bioprinting.

3D bioprinting holds great potential for use in tissue engineering to treat degenerative joint disorders, such as osteoarthritis. However, there is a lack of multifunctional bioinks that can not only support cell growth and differentiation, but also offer protection to cells against injuries caused by the elevated oxidative stress; this conditions is a common characteristic of the microenvironment of the osteoarthritis disease. To mitigate oxidative stress-induced cellular phenotype change and malfunction, an anti-oxidative bioink derived from an alginate dynamic hydrogel was developed in this study.

Micro/Nano-Fabrication of Flexible Poly(3,4-Ethylenedioxythiophene)-Based Conductive Films for High-Performance Microdevices.

With the increasing demands for novel flexible organic electronic devices, conductive polymers are now becoming the rising star for reaching such targets, which has witnessed significant breakthroughs in the fields of thermoelectric devices, solar cells, sensors, and hydrogels during the past decade due to their outstanding conductivity, solution-processing ability, as well as tailorability. However, the commercialization of those devices still lags markedly behind the corresponding research advances, arising from the not high enough performance and limited manufacturing techniques. The conductivity and micro/nano-structure of conductive polymer films are two critical factors for achieving high-performance microdevices.

Affective or cognitive interpersonal emotion regulation in couples: an fNIRS hyperscanning study.

Sadness regulation is crucial for maintaining the romantic relationships of couples. Interpersonal emotion regulation, including affective engagement (AE) and cognitive engagement (CE), activates social brain networks. However, it is unclear how AE and CE regulate sadness in couples through affective bonds.