ND-FISH with New Oligo Probes for Chromosome Identification of Revealing Karyotypic Variation and Divergence of Asteraceae Species.

Chicory ( L., 2n = 18), belonging to the Asteraceae family, exhibits significant edible, medicinal, and pasture values. Moderate research has been performed on identifying Chicory species' chromosomes using fluorescence in situ hybridization (FISH) and C-banding.

The response mechanism analysis of knockout strain to levulinic acid in Saccharomyces cerevisiae.

Levulinic acid, a hydrolysis product of lignocellulose, can be metabolized into important compounds in the field of medicine and pesticides by engineered strains of Saccharomyces cerevisiae. Levulinic acid, as an intermediate product widely found in the conversion process of lignocellulosic biomass, has multiple applications. However, its toxicity to Saccharomyces cerevisiae reduces its conversion efficiency, so screening Saccharomyces cerevisiae genes that can tolerate levulinic acid becomes the key.

Genome-wide identification of resistance genes and response mechanism analysis of key gene knockout strain to catechol in .

Engineering for biodegradation and transformation of industrial toxic substances such as catechol (CA) has received widespread attention, but the low tolerance of to CA has limited its development. The exploration and modification of genes or pathways related to CA tolerance in is an effective way to further improve the utilization efficiency of CA. This study identified 36 genes associated with CA tolerance in through genome-wide identification and bioinformatics analysis and the knockout strain (Δ) is the most sensitive to CA.

Genome-wide identification of resistance genes and cellular analysis of key gene knockout strain under 5-hydroxymethylfurfural stress in Saccharomyces cerevisiae.

In bioethanol production, the main by-product, 5-hydroxymethylfurfural (HMF), significantly hinders microbial fermentation. Therefore, it is crucial to explore genes related to HMF tolerance in Saccharomyces cerevisiae for enhancing the tolerance of ethanol fermentation strains. A comprehensive analysis was conducted using genome-wide deletion library scanning and SGAtools, resulting in the identification of 294 genes associated with HMF tolerance in S.

Bamboo lignocellulose degradation by gut symbiotic microbiota of the bamboo snout beetle .

Background: Gut symbiotic microbiota plays a critical role in nutrient supply, digestion, and absorption. The bamboo snout beetle, , a common pest of several bamboo species, exhibits high lignocellulolytic enzyme activity and contains various CAZyme genes. However, to date, no studies have evaluated the role of gut symbiotic microbiota of the snout beetle on bamboo lignocellulose degradation.

Transcriptome analysis of Cyrtotrachelus buqueti in two cities in China.

In order to reduce the Cyrtotrachelus buqueti population, which is a serious pest in the bamboo industry, a range of approaches is required, which will be dependent on diverse gene expression influenced by environmental factors. In this study, samples from two regions of China, Muchuan in Sichuan Province and Chishui in Guizhou Province, were investigated through RNA-seq. Approximately 44 million high-quality reads were generated and 94.