[Evaluation of PIMA analyzer detecting CD4 cell count of venous and capillary blood in HIV-infected individuals].

Objective: This study is aimed at evaluating the utility of the portable CD4 analyzers (PIMA).

Methods: The paired finger prick blood (25 µl) and 5 ml venous blood samples were collected from 196 HIV infected patients, who came to Yunnan CDC voluntary counseling and testing (VCT) clinic for CD4 test services, from May to August, 2012. The absolute CD4 cell counts were measured by PIMA (using venous and finger-prick blood) and by Calibur (using venous blood) as the reference.

[Early detection of HIV infection with Dried Blood Spot testing among infants in Yunnan province].

Objective: To explore the application of Dried Blood Spot (DBS) testing for early detection of HIV infection among infants.

Methods: All of the infants aged between 6 weeks and 18 months and born by HIV positive mothers from 14 Maternity and Child Health Care Hospitals in Kunming, Dali, Dehong, Lincang of Yunnan province were investigated from 2010 to 2011. By using DBS and Roche HIV-1 DNA test techniques, 286 infants were tested for HIV early diagnosis and compared with HIV antibody results of 18 months infants.

[A dynamic analysis on incidence and trend of HIV-1 epidemics among intravenous drug users, attendants at the STD clinics and pregnant women in Yunnan province, China].

Objective: BED-CEIA assay was used in HIV/AIDS surveillance sentinel sites to identify recent HIV-1 infection, to estimate HIV-1 incidence and to understand the epidemic trends in Yunnan province.

Methods: Serum specimens were collected from IDUs in sentinel sites, attendants in STD clinics and pregnant women under a cross sectional study from 2000 to 2007. Specimens confirmed as HIV-1 positive were tested with BED-CEIA to find recent HIV-1 infection, then the annual HIV-1 incidence for each group was calculated and the trends of HIV-1 incidence observed.

[The analysis of human immunodeficiency virus-1 subtypes in Yunnan province].

Objective: To analyze the geographical distribution and risk factors of HIV-1 subtypes in Yunnan province.

Methods: Blood samples from 1319 HIV positives were collected in Yunnan Province from 2001 to 2006. The nested polymerase chain reaction was used to amplify the gag (p24)-protease fragments from RNA extracted from plasma or sera.