Gambogic acid induces G0/G1 arrest and apoptosis involving inhibition of SRC-3 and inactivation of Akt pathway in K562 leukemia cells.

Gambogic acid (GA), a major active component of gamboge, exhibits potent anticancer activity in many kinds of cancer cells. However, the anticancer mechanism of GA is not clearly understood. Here we showed that GA could cause growth inhibition, induce the G0/G1 phase cell cycle arrest and apoptosis in human chronic myelogenous leukemia cell line K562 cells.

Involvement of SRC-3 in deguelin-induced apoptosis in Jurkat cells.

The aim of the study was to investigate the anticancer effects and the molecular mechanisms of deguelin on Jurkat cells. Cell viability was assessed by MTT assay. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay and transmission electron microscopy were used to detect cell apoptosis.

[Effects of gambogic acid on the regulation of nucleoporin Nup88 in HL-60 cells].

Objective: To investigate the effect of gambogic acid (GA) on cell proliferation and induction of apoptosis in HL-60 cells in vitro, as well as the regulation of nucleoporin Nup88 to explore the relationship between them.

Methods: The effect of GA on the growth of HL-60 cells was determined by MTU assay. Apoptosis was detected with Hoechst 33258 staining and annexin-V FITC/PI double-labeled flow cytometry.

[Isolation of Mycoplasma penetrans and detection of the levels of serum IL-6 and TNF-alpha in the blood of patients with autoimmune disease].

Aim: To investigate the isolation of Mycoplasma penetrans from the blood of autoimmune disease patients and to evaluate the levels of serum interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in patients with autoimmune disease (AID).

Methods: M. penetrans was isolated and cultured from the blood specimens of 44 patients with AID.