Publications by authors named "Wen-ting Tan"

RNA structure is critical for multiple steps in gene regulation. However, how the structures of transcripts differ both within and between individual cells is unknown. Here we develop a SHAPE-inspired method called single-cell structure probing of RNA transcripts that enables simultaneous determination of transcript secondary structure and abundance at single-cell resolution.

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Objectives: To investigate the relationship between systemic inflammatory response and short-term mortality in patients with non-cirrhotic chronic severe hepatitis (CSH) by using several indicators of inflammation including neutrophil-to-lymphocyte ratio (NLR), neutrophil (NEU), white blood cell (WBC), platelet-to lymphocyte ratio (PLR), and monocyte-to-lymphocyte ratio (MLR).

Methods: Data were collected from two prospectively enrolled CATCH-LIFE noncirrhotic cohorts. Cox regression analysis was used to investigate the association between systemic inflammatory biomarkers and 90-day liver transplant (LT)-free mortality.

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Article Synopsis
  • Autoimmune liver disease (AILD) has been found to have a prevalence of 9.3% among cirrhotic patients in China suffering from acute decompensation (AD), based on a study of nearly 2,600 patients.
  • The study revealed that patients with AILD experienced a significantly lower rate of acute-on-chronic liver failure (ACLF) compared to those with cirrhosis from other causes, with 28-day and 90-day mortality rates in AILD-related ACLF patients being 43.8% and 80%, respectively.
  • Key risk factors for increased mortality within 90 days included total bilirubin levels, hepatic encephalopathy, and blood urea nitrogen, while the specific type of
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The distribution, dynamics, and function of RNA structures in human development are under-explored. Here, we systematically assayed RNA structural dynamics and their relationship with gene expression, translation, and decay during human neurogenesis. We observed that the human ESC transcriptome is globally more structurally accessible than differentiated cells and undergoes extensive RNA structure changes, particularly in the 3' UTR.

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Current methods for determining RNA structure with short-read sequencing cannot capture most differences between distinct transcript isoforms. Here we present RNA structure analysis using nanopore sequencing (PORE-cupine), which combines structure probing using chemical modifications with direct long-read RNA sequencing and machine learning to detect secondary structures in cellular RNAs. PORE-cupine also captures global structural features, such as RNA-binding-protein binding sites and reactivity differences at single-nucleotide variants.

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Article Synopsis
  • Portal vein thrombosis (PVT) is a serious issue in cirrhosis patients and is more common in those experiencing acute decompensation (AD), with a prevalence of 9.36% compared to 5.24% in those without AD.
  • Recent PVT was found in 63.37% of patients with cirrhosis and AD, and these patients also had higher rates of variceal bleeding and elevated D-dimer levels.
  • Despite the higher incidence of bleeding and complications, the one-year mortality rate was similar between patients with and without PVT, highlighting the need for preventive measures and regular screenings in vulnerable cirrhosis patients.
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β-defensin family plays a role in host defense against viral infection, however its role in HCV infection is still unknown. In this study, we demonstrated that β-defensin 1 was significantly reduced in HCV-infected liver specimens. Treatment with interferon and ribavirin upregulated β-defensin-1, but not other β-defensin tested, with the extent and duration of upregulation associated with treatment response.

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Article Synopsis
  • - The study investigates how the androgen receptor (AR) pathway may affect chronic hepatitis B (CHB) and acute liver failure (ALF) in males, focusing on the impact of a specific genetic variation (CAG repeat) in the AR gene.
  • - Researchers analyzed 378 male CHB patients with ALF and 441 asymptomatic HBV carriers, finding that higher CAG repeat numbers significantly increased the risk of ALF and that testosterone levels varied during hepatitis flare and liver failure phases.
  • - The results indicate that while serum testosterone levels were higher during hepatitis flare, they dropped sharply during ALF, and that men with the CAG variant had lower testosterone levels, linking genetic factors to susceptibility to HBV-related liver
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The organic anion transporting polypeptide 1B1 (OATP1B1, encoded by SLCO1B1) plays an important role in the transport of endogenous and xenobiotic compounds, such as bile acids and rifampin. In this study, the association between OATP1B1 polymorphisms and rifampin hepatotoxicity was investigated using integrated population genetic analysis and functional studies. A total of 273 unrelated patients treated with rifampin were recruited.

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Background: The shift in the distribution of HCV genotypes could have a significant impact on practical medical issues.

Objectives: To describe the recent distribution and evolution of HCV genotypes in southwest China.

Study Design: HCV genotypes were determined by nucleotide sequencing of the CE1 regions followed by phylogenic analysis with the published HCV genotype.

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Objective: To investigate the dynamic correlation between pre-S1 antigen, pre-S2 antigen and HBV DNA in the serum of chronic hepatitis B (CHB) patients undergoing nucleoside analogue therapy.

Methods: 12 CHB patients with transient virological response after lamivudine treatment, and 20 patients treated with adefovir for 5 years were recruited in this study. Serum samples were collected at four time points when HBV DNA fluctuated sharply during lamivudine treatment, and at 0, 8, 12, 28, 52, 104, 156, 208, 260 weeks following adefovir treatment.

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