A new strictosamide-based indolo[2,3-a]quinolizidine alkaloid and two new lignans from the stem bark of Smith.

Phytochemical investigation of Smith. (Rubiaceae), an African ethnomedicinal plant, yielded a new indolo[2,3-a]quinolizidine alkaloid, nauclealatimide , two previously unknown lignans named nauclealignan A and B , as well rhemaneolignan B and spicatolignan A from the hydromethanolic extract obtained after ultrasonication of the stem bark. Their chemical structures were elucidated using IR, NMR (1D and 2D) and HRESIMS spectral evidence as well as comparisons with existing literature data.

3-Hydroxyneocryptolepine, a new indoloquinoline alkaloid from the roots of (Lindl.) Schltr.

Phytochemical investigations of the African ethnomedicinal plant (Lindl.) Schltr. (Apocynaceae) have yielded only a small number of rare naturally occurring indoloquinoline alkaloids.

Sesquiterpene lactones from Tithonia diversifolia with ferroptosis-inducing activities.

Eight previously undescribed sesquiterpene lactones (1-8), together with six known ones (9-14) were isolated from the aerial parts of Tithonia diversifolia (Hemsl.) A. Gray.

Ionic regulation whey protein composition on membrane fouling during ultrafiltration process analyzed by HPLC.

In order to meet the market demand and avoid the increase of operation amount and cleaning cost in the process of ultrafiltration, it is particularly important to find more practical and efficient methods to control and improve membrane fouling. In this study, the ions in the ultrafiltration process were regulated to affect membrane surface proteins composition (lactoferrin, α-lactalbumin, β-lactoglobulin A and β-lactoglobulin B) and delay membrane fouling. It was found that Na (21 mmol/L), Zn (0.

Five undescribed abietane-type diterpenes of Callicarpa macrophylla.

A phytochemical investigation of the medicinal plant Callicarpa macrophylla resulted in the characterization of two rare rearrangement abietane-type diterpenoids, macrophypene F-G (1-2), and three abietane diterpenoids, named macrophypene H-J (3-5). Additionally, five known diterpenoids (6-10) were identified. The structures of the newly discovered compounds were fully established through extensive analysis of HRESIMS, 1D and 2D NMR data.

Novel indoloquinoline alkaloid glycosides from (Lindl.) Schltr.

To explore the phytochemistry of the African ethnomedicinal plant (Lindl.) Schltr. (Apocynaceae) for rare indoloquinoline alkaloids, two novel indoloquinoline alkaloid glycosides, namely Cryptospirosanguine A () and B (), were isolated from an ethanolic extract of the root.

Five undescribed guanidine alkaloids from Plumbago zeylanica.

Five undescribed guanidine alkaloids, plumbagines HK (1-4) and plumbagoside E (5), as well as five known analogues (6-10) were isolated from the roots of Plumbago zeylanica. Their structures were established by extensive spectroscopic analyses and chemical methods. In addition, 1-10 were accessed their anti-inflammatory activities by measuring nitric oxide (NO) concentrations in LPS-induced RAW 264.

The mechanism of whey protein and blueberry juice mixed system fermented with Lactobacillus inhibiting Escherichia coli during storage.

The aim of this study was to identify the antimicrobial effect and mechanism of whey protein and blueberry juice mixed systems fermented with Lactobacillus against Escherichia coli during storage. The whey protein and blueberry juice mixed systems were fermented with L. casei M54, L.

The effect of ion environment changes on retention protein behavior during whey ultrafiltration process.

The factors affecting membrane fouling are very complex. In this study, the membrane fouling process was revealed from the perspective of ion environment changes, which affected the whey protein structure during ultrafiltration. It was found that the concentrations of Ca and Na were overall increased and the concentrations of K, Mg and Zn were decreased at an ultrafiltration time of 11 min, which made more hydrophilic groups buried inside and increased the content of α-helix, leading to more protein aggregation.

Five glycosylated phenolic derivatives from the bark of Thunb. and their anti-inflammatory activities.

Five new glycosylated phenolic derivatives, rotundosides A-E (-), and three known glycosides (-) were isolated from the 95% alcohol extract of the bark of . Their structures were elucidated by extensive spectroscopic analysis and comparison with the literature data. All new compounds possessed a [5--()-caffeoyl]--D-apiofuranosyl-(1→6)--D-glucopyranosyl group.

Calysepins I-VII, Hexasaccharide Resin Glycosides from and Their Cytotoxic Evaluation.

Seven new hexasaccharide resin glycosides, named calysepins I-VII (-), with 27-membered rings, were obtained from the aerial parts of . Their structures with absolute configuration were established on the basis of spectroscopic data interpretation analysis and the use of chemical methods. They were defined as hexasaccharides composed of one d-quinovose, four d-glucose, and one l-rhamnose unit, and their sugar moieties were partially acylated by (2)methylbutanoic acid in - and (2,3)-nilic acid in - and , which mainly differed at the positions of acylation.

Structural and compositional changes of whey protein and blueberry juice fermented using or during fermentation.

This study aimed to improve the stability of the anthocyanins and phenolic acids of blueberry by forming hydrogen bonds or hydrophobic interactions with whey protein using lactic acid fermentation. The effects of the initial pH on the characteristics of the whey protein and blueberry juice system fermented using and were investigated. The color and total phenol and anthocyanin contents of the blueberry juice and whey protein system became stable after fermentation using and .

The effect of composite enzyme catalysis whey protein cross-linking on filtration performance.

In this study, enzymatic cross-linked whey protein coupling ultrafiltration was used to reduce membrane fouling and increase whey protein recovery rate. The filtration efficiency and protein interaction with the membrane surface were investigated. The results showed that the protein recovery rate and relative flux of transglutaminase catalysis protein followed by tyrosinase each increased by approximately 30% during ultrafiltration.

Anti-inflammatory sesquiterpene dimers and diterpenes from the aerial part of .

Phytochemical investigation on the 95% alcohol extract of the aerial part of led to the isolation of three new compounds, inulanolides F-G () and 17-carboxaldehyde-ent-kaur-18-oic acid (), together with four known compounds (). The structures of new compounds were elucidated by using spectroscopic data. Most of the isolated compounds showed significant anti-inflammatory activities.

Neolinulicin A and B from Inula japonica and their anti-inflammatory activities.

A phytochemical study performed on Inula japonica led to isolation of a new 1,10-seco-sesquiterpene dimer Neolinulicin A (1) and 1,10-seco-sesquiterpene Neolinulicin B (2), together with nine known sesquiterpenes (3-11). Among them, Neolinulicin A (1), which has a new carbon skeleton, was a Diels-Alder [4 + 2] adduct of two sesquiterpene moieties. Their structures were established by extensive spectroscopic analysis.

Teratopyrones A-C, Dimeric Naphtho-γ-Pyrones and Other Metabolites from sp. AK1128, a Fungal Endophyte of .

Bioassay-guided fractionation of a cytotoxic extract derived from a solid potato dextrose agar (PDA) culture of sp. AK1128, a fungal endophyte of , afforded three new naphtho-γ-pyrone dimers, teratopyrones A-C (-), together with five known naphtho-γ-pyrones, aurasperone B (), aurasperone C (), aurasperone F (), nigerasperone A (), and fonsecin B (), and two known diketopiperazines, asperazine () and isorugulosuvine (). The structures of - were determined on the basis of their spectroscopic data.

23,24-Dihydrocucurbitacin B promotes lipid clearance by dual transcriptional regulation of LDLR and PCSK9.

23,24-Dihydrocucurbitacin B (designated as C95 in this article) is a cucurbitane triterpenoid that has been shown to possess a variety of pharmacological activities, such as anti-inflammatory and anti-HIV-1 activities etc. In this study, we investigated the effects of 23,24-dihydrocucurbitacin B on lipid regulation. We showed that 23,24-dihydrocucurbitacin B (1-5 μM) dose-dependently promoted DiI-LDL uptake in HepG2 cells by upregulating low-density lipoprotein receptor (LDLR) protein.

Whey protein membrane processing methods and membrane fouling mechanism analysis.

Whey is a byproduct with nutritional value and high organic and saline content. It is an important source of organic contamination in dairy industry. In this paper, we gave an overview of the current use of membrane materials and membrane processing in cheese whey protein recovery and discussed recent developments in membrane technology.

Three new dibenzofurans from Mill.

Phytochemical investigation of Mill. collected in Xinjiang province, China, led to the isolation and identification of three new dibenzofurans (-) along with one known compound (). Their structures were elucidated based on HRESIMS, spectroscopic data (IR, UV, 1D, 2D NMR) and X-ray diffraction analysis.

New flavonoid glycosides from seeds of .

is the most popular traditional Uighur medicines used for vitiligo. The chemical investigation of the seeds of led to the isolation of three new flavonoid glycosides (Vernosides A-C). Their structures were determined by comprehensive analysis of spectroscopic data including 1D and 2D NMR and HRMS data.

Optimization of transglutaminase (TG) immobilization on the surface of polyethersulfone ultrafiltration membrane and its characteristics in a membrane reactor.

The process of microbial transglutaminase (TG) covalent immobilization on an ultrafiltration membrane surface, the optimum immobilization conditions and the characteristics of the enzymatic membrane in a reactor were investigated. The process of TG immobilization on polyethersulfone (PES) membrane surfaces was analyzed by Fourier transform infrared and X-ray photoelectron spectroscopy. The optimal condition for TG immobilization was in pH 5.

Cytotoxic and other withanolides from aeroponically grown Physalis philadelphica.

Eleven withanolides including six previously undescribed compounds, 16β-hydroxyixocarpanolide, 24,25-dihydroexodeconolide C, 16,17-dehydro-24-epi-dioscorolide A, 17-epi-philadelphicalactone A, 16-deoxyphiladelphicalactone C, and 4-deoxyixocarpalactone A were isolated from aeroponically grown Physalis philadelphica. Structures of these withanolides were elucidated by the analysis of their spectroscopic (HRMS, 1D and 2D NMR, ECD) data and comparison with published data for related withanolides. Cytotoxic activity of all isolated compounds was evaluated against a panel of five human tumor cell lines (LNCaP, ACHN, UO-31, M14 and SK-MEL-28), and normal (HFF) cells.