[The study of the secondary metabolites from fungus Paecilomyces sp].

Objective: To get active secondary metabolites from the fungus Paecilomyces sp..

Methods: The strain Paecilomyces sp.

[The secondary metabolites of the HS-3 Alternaria sp. fungus associated with holothurians].

Objective: The metabolites of HS-3 associated with holothurians were studied, which was identified by molecular biology as Alternaria sp..

Methods: The holothurians were gathered from the Sea of Zhifu Islet, Shandong Province.

[Study on the second metabolisms from fungus HS-1 Epicoccum spp. from the sea cucumber in Yellow Sea].

Objective: To get active metabolites from the microbes associated with sea cucumber.

Methods: Fungus was isolated from the sea cucumber, and the species was identified by molecular biology, and then was cultivated in GYP medium, and the metabolites were got by chromatography. Their structures were identified by comprehensive spectroscopic methods.

[The secondary metabolites of the mangrove endophytic fungi ZZF13 and Guignardia sp. 4382 from the South China Sea].

Objective: The secondary metabolites of the fungus ZZF13 isolated from the leaves of the mangrove sample Kandelia candel in Zhanjiang and Guignardia sp. 4382 isolated from bark of Kandelia candel (endophyte) of Mai Po, Hong Kong were studied.

Methods: The compounds were isolated by siliga gel, and their structures were identified by IR, MS and NMR.

[Studies on the purification of water-soluble holothurian glycosides from Apostichopus japonicus and their tumor suppressing activity].

To obtain water-soluble holothurian glycosides with high tumor suppressing activities from Apostichopus japonicus, macroporous resin, silica gel and gel-filtration column chromatograghy were used to purify the water-soluble holothurian glycosides, and their tumor suppressing activity and inducing apoptosis of tumor cells were examined in this study. The 70% ethanol fraction of macroporous resin column, the pSC-2 and pSC-3 fractions from silica gel column showed very strong tumor suppressing activity towards HeLa cells, A-549 lung cancer cells, SGC-7901 stomach cancer cells and Bel-7402 liver cancer cells. SC-2 and SC-3 fraction purified from Sephadex LH-20 gel-filtration column chromatography, with a purity above 99.

Antioxidant activity of chito-oligosaccharides on pancreatic islet cells in streptozotocin-induced diabetes in rats.

Aim: To investigate the antioxidant activity of chito-oligosaccharides (COSs) on pancreatic islet cells in diabetic rats induced by streptozotocin.

Methods: The antioxidant effect of COSs on pancreatic islet cells was detected under optical microscopy and with colorimetric assay and gel electrophoresis. The activities of glutathione peroxidase and superoxide dismutase, total antioxidant capacity, and content of malondialdehyde in serum and tissue slices of pancreas were examined after 60 d to determine the effect of COSs in streptozotocin-induced diabetes in rats.

[Research on preparation of Shandantong sustained-release tablets].

Objective: To optimize the preparation of Shandantong sustained-release tablets.

Methods: We used the orthogonal experiment to optimize the preparation and determined the contents of Shanzha total flavonoids and tanshinone. Involved technology and hydrophilicity skeleton materials were adopted to prepare the sustained-release tablets.

[A new textual research on origin of monascus rice].

Objective: To explore the origin of Monascus Rice.

Methods: The relative literature were investigated and classified.

Results: It was verifiable that the record of Monascus Rice in Chu Xue Ji ([Chinese characters: see text]), a subject-based reference book compiled by Xu Jian in the Tang Dynasty, was excerpted from the metrical verse Qi Shi ([Chinese characters: see text]) written by Wang Can in the Dong Han Dynasty.