Effects of CD4 T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

The present study was designed to investigate the effects of T cells on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). BMMSCs were co-cultured with CD4 T cells that had been pretreated with anti-TNF-α or controls and were derived from ovariectomized (OVX) mice or sham control mice. MTT was used to assess the proliferative ability of BMMSCs and flow cytometry was used to analyze the BMMSC cell cycle.

Two Nanometer-Sized High-Nuclearity Homometallic Bromide Clusters (MBr) (M = Cu, Ag): Syntheses, Crystal Structures, and Efficient Adsorption Properties.

The spontaneous formation of discrete spherical nanosized molecules is ubiquitous in nature; however, the actual structural imitation of such high-symmetry polyhedra from the edge sharing of regular polygons has still proved to be elusive. Herein, two high-nuclearity metal clusters, namely (TTB)·MBr (M = Cu (), Ag (), TTB·Br = 1, 3, 5-tris(triethylammoniomethyl)benzene tribromide), have been rationally and solvothermally synthesized and structurally characterized. Single-crystal X-ray analysis confirmed that has 4̅3 symmetry with a (CuBr) anion shell enwrapping a CuBr tetrahedron and has 4̅3 symmetry with a [AgBr] anion shell enwrapping a Br pyramid.

[Epidemiological investigation of blood transfusion - induced malaria caused by an imported case with infection].

Objective: To analyze a case infected with due to blood transfusion in Taizhou City, so as to provide scientific evidence for malaria control.

Methods: The epidemiological investigation of a case with malaria was conducted, and the blood donors'blood samples were collected and detected in laboratory to identify the origin of the infection.

Results: Microscopy detected in the blood samples from a 64-year-old male inpatient with nephropathy, and malaria was diagnosed.

Spontaneous Remission of Subcutaneous Scedosporiosis Caused by Scedosporium dehoogii in a Psoriatic Patient.

To date, only one case of post-traumatic endophthalmitis caused by Scedosporium dehoogii has been reported, but its contamination or colonization might not be precluded due to the absence of pathogenic isolation and/or pathological examination. We report the first case to our knowledge of S. dehoogii-induced subcutaneous scedosporiosis in a psoriatic patient.

Kerion and Tinea Corporis Caused by Rabbit-Derived Trichophyton interdigitale in Three Siblings and One Consulting Doctor Using β-Tubulin Gene to Identify the Pathogen.

Trichophyton interdigitale is generally deemed as an anamorph of Arthroderma vanbreuseghemii based on internal transcribed spacer (ITS) sequencing, but recently their anamorph/teleomorph connection should be cautioned based on β-tubulin phylogeny. We report three siblings and one consulting doctor who developed kerion and tinea corporis after contact with domestic rabbits. Seven same strains were isolated from four patients and three regions of a sick rabbit.

Cutaneous Chromoblastomycosis Caused by Veronaea botryosa in a Patient with Pemphigus Vulgaris and Review of Published Reports.

Chromoblastomycosis and phaeohyphomycosis represent two poles of a disease spectrum caused by melanized fungi. Veronaea botryosa belongs to a small genus of saprobic fungi that occasionally cause human infections. To date, 11 cases of V.

Comparison of modified Chicago sky blue stain and potassium hydroxide mount for the diagnosis of dermatomycoses and onychomycoses.

The diagnostic value of modified Chicago sky blue (CSB) stain and potassium hydroxide (KOH) mount for superficial mycoses was compared using fungal culture as gold standard. The sensitivity and screening time of the CSB stain were superior to the KOH mount. The CBS stain is simple, quick and reliable for diagnosing superficial mycoses.

Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China.

There is limited information regarding the molecular epidemiology and antifungal susceptibilities of Candida isolates using the Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18-50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and the D1/D2 domain.

PKG and NHR-49 signalling co-ordinately regulate short-term fasting-induced lysosomal lipid accumulation in C. elegans.

Lysosomes act as terminal degradation organelles to hydrolyse macromolecules derived from both the extracellular space and the cytoplasm. In Caenorhabditis elegans fasting induces the lysosomal compartment to expand. However, the molecular and cellular mechanisms for this stress response remain largely unclear.

[Construction of a red fluorescent shuttle vector controlled by recA operon promoter of Streptococcus mutans].

Objective: To construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans.

Methods: The promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred.

Results: pLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed.

Brain abscess caused by Cladophialophora bantiana in China.

A case of a 38-year-old male farmer with a brain abscess caused by Cladophialophora bantiana is described. He had a 2 year history of non-insulin-dependent diabetes and myelodysplastic syndrome. A cranial computed tomography scan demonstrated a hypodense ring lesion with peripheral oedema and a midline shift in the left frontal lobe.

Experimental pathogenicity of a clinical isolate of Trichosporon dermatis in a murine model.

The pathogenicity of Trichosporon dermatis isolated from skin lesions of a patient has been examined in mice. Balb/c mice were treated with two intraperitoneal injections of 100 mg/kg cyclophosphamide on days 4 and 1 and one subcutaneous injection of 10 mg/kg dexamethasone on day 1 pre-inoculation, and then challenged with 0.2 ml T.

Cutaneous phaeohyphomycosis of foot caused by Curvularia clavata.

A case of cutaneous lesions on the left foot caused by Curvularia clavata in a 64-year-old immunocompetent man is described. Fungal elements were found in the exudate and biopsy specimen. The isolate was identified as C.

Biased genotype distributions of Candida albicans strains associated with vulvovaginal candidosis and candidal balanoposthitis in China.

Background: Vulvovaginal candidosis (VVC), which is most frequently caused by Candida albicans, is one of the most common vaginal infections and is a common problem worldwide. Despite the fact that extensive epidemiological studies have been performed, what triggers VVC, especially recurrence of the infection, is still uncertain.

Methods: Genotypes of C.

Granulomatous skin infection caused by Malassezia pachydermatis in a dog owner.

Background: Malassezia pachydermatis is part of the normal cutaneous microflora of dogs and many other mammals. M pachydermatis has not yet been reported as an agent that causes skin infection in humans, although it has been found to cause fungemia and other nosocomial infections in preterm newborns and immunocompromised adults.

Observations: Malassezia pachydermatis was isolated from the facial granuloma of a healthy woman and her dog's skin scrapings and cerumen.