[Electroacupuncture improves intestinal function by down-regulating expression of 5-HTR and c-fos proteins in the hypothalamus and colon tissues via brain-gut axis in rats with functional diarrhea].

Objective: To observe the effect of electroacupuncture (EA) on expression of 5-HT receptor (5-HTR) and c-fos proteins in the hypothalamus and colon tissues in functional diarrhea (FD) rats so as to explore its underlying mechanisms in improving intestinal function via brain-gut axis.

Methods: A total of 40 SD rats were randomized into blank control, model, EA Tianshu (ST25)-Dachangshu (BL25, ST25-BL25) and EA Quchi (LI11)- Shangjuxu (ST37, LI11-ST37) groups. The FD model was established by gavage of Folium Sennae (10 mg/kg) and constraint immobilization once daily for 29 days.

Silibinin exerts antidepressant effects by improving neurogenesis through BDNF/TrkB pathway.

Classic antidepressants benefit depression patients partially by improving neurogenesis and/or brain-derived neurotrophic factor (BDNF)/TrkB pathway which were impaired in depression. In this study, we demonstrated that Silibinin (SLB), a polyphenolic flavanoid from Silybum marianum, ameliorated reserpinized mouse depressant-like behaviors. The antidepressants of SLB administration was associated with increased neural stem cells (NSCs) proliferation and further confirmed in BDNF/TrkB signaling transduction.

[Effect of "Xiusanzhen" on hippocampal muscarinic cholinergic receptor activity in Alzheimer disease rats].

Objective: To explore the effect of "Xiusanzhen" [electroacupuncture (EA) of bilateral "Yingxiang" (LI 20) and "Yintang" (EX-HN 3)] on activities of hippocampal muscarinic cholinergic receptor (mAChR) and the involved neural path in Alzheimer Disease(AD)rats.

Methods: Forty Sprague Dawley rats were randomly divided into normal control, model, olfactory nerve severing (ONS)-EA of "Xiusanzhen" (ONS-EA) and EA of "Xiusanzhen" (EA) groups. AD model was established by intra-hippocampal injection (AP: 3.

[Effect of "Xiusanzhen"on expression of hippocampal Bcl-2 and Bax proteins in Alzheimer disease rats].

Objective: To observe the effect of electroacupuncture (EA) of "Xiusanzhen" [3 acupoints, i.e., bilateral "Yingxiang" (LI 20) and "Yintang" (EX-HN 3)] on expression of B-cell lymphoma/leukemia-2 (Bcl-2, an antiapoptosis protein) and Bax (a protein for promoting apoptosis) in the hippocampus in Alzheimer disease (AD) rats, so as to explore its clinical mechanisms underlying relieving AD.

Study on perfume stimulating olfaction with volatile oil of Acorus gramineus for treatment of the Alzheimer's disease rat.

Objective: To probe into the therapeutic effect of perfume stimulating olfaction with volatile oil of Acorus Gramineus on the Alzheimer's disease (AD) rat.

Methods: Totally 50 adult SD rats, male,weighing 300 +/- 10 g, were randomly divided into 5 groups, normal group (group A), olfactory nerve severing model group (group B), AD model group (group C), AD model plus perfume stimulation group (group D), AD model olfactory nerve severing plus perfume stimulation group (group E), 10 rats in each group. After perfume stimulation, Morris maze test was conducted for valuating the learning and memory ability; Malondaldehyde (MDA) content, and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the brain, and the brain weight were detected.

[Micro-acupuncture system in the hairline region of the scalp].

Studying on the acupoints in the hairline region of the scalp, it is discovered that a group of acupoints are orderly arranged in the hairline region of the scalp. Furthermore, the arrangement is highly consistent with the meridian route of zang-fu organs. Manipulating needles at acupoints in the hairline regions of the scalp may treat certain diseases, including insomnia, migraine, and Alzheimer disease.

[Effect of electroacupuncture on cerebral cortex ultrastructure in rats with cerebral ischemia-reperfusion injury].

Objective: To observe the effect of electroacupuncture (EA) of "Baihui" (GV 20) and "Dazhui" (GV 14) on the ultrastructure of cerebral cortex in rats with cerebral ischemia reperfusion (CI/R) injury.

Methods: Fifteen SD rats were equally randomized into sham-operation (sham), model and EA groups. CI/R model was established by right middle cerebral artery occlusion for 1 h and reperfusion for 24 h.

[Effect of "Xiusanzhen" on learning-memory ability and hippocampal somatostatin and arginine vesopressin contents in vascular dementia rats].

Objective: To observe the effect of electroacupuncture (EA) of "Xiusanzhen" [bilateral "Yingxiang" (LI 20) + "Yintang" (GV 29)] on learning-memory ability and hippocampal somatostatin (SS) and arginine vasopressin (AVP) contents in vascular dementia (VD) rats.

Methods: Forty SD rats were randomly divided into control, VD model, VD plus olfactory bulb lesion (OBL, destroyed by electro-coagulation) and EA groups, with 10 cases in each. VD model was established by 4-vessel occlusion.

[Effect of "Xiusanzhen" on learning-memory ability and hippocampal ChAT and AChE activity in Alzheimer disease rats].

Objective: To explore the effect of "Xiusanzhen" [bilateral "Yingxiang" (LI 20) + "Yintang" (EX-HN 3)] on learning-memory ability and choline acetyl transferase (ChAT) and acetyl cholinesterase (AChE) activity of hippocampus tissue in Alzheimer Disease (AD) rats.

Methods: A total of 40 Sprague Dawley rats were randomly divided into control, AD model, AD + olfactory-bulb lesion (OBL, induced by electrocoagulation method) + EA and "Xiusanzhen" (AD + EA) groups. AD model was established by 4-vessel occlusion.

[Involvement of melatonin in the adjusting effect of electroacupuncture in resisting oxygen stress in cerebral ischemia-reperfusion injury rats].

Objective: To explore the effect of electroacupuncture (EA) in resisting acute cerebral ischemia-reperfusion injury (CI-RI) via anti-oxidation of melatonin (MT).

Methods: A total of 52 male SD rats were randomized into Sham-operation (sham, n=8), CI-RI (model, n=8), EA (n=8), MT (3.2 mg/Kg, i.

[Clinical investigation on electroacupuncture treatment of vascular dementia with "Xiusanzhen"].

Objective: To observe the effect of electroacupuncture (EA) in the treatment of vascular dementia (VD) so as to explore an effective therapy for it.

Methods: Sixty cases of VD were randomly divided into acupuncture group and medication (Duxil, 40 mg/time, 2 times/d for 10 weeks) group, with 30 cases in each group. "Xiusanzhen" means that two acupuncture needles were penetrated subcutaneously from bilateral Yingxiang (LI 20) to Shangyingxiang (EX-HN 8) respectively, and the 3rd needle was penetrated from the midpoint between two Yangbai (GB 14) through Yintang (EX-HN 3) to the nasal root, followed by applying EA stimulation to the acupoints.

[Study on the morphological evidence of five-shu-point].

Objective: To search for the morphological evidence of five-shu-point so as to explain the theory of the five-shu-point more reasonably.

Methods: Carefully study on Yellow Emperor's Internal Classic and compare with the modern anatomic knowledge.

Conclusion: The theory of five-shu-point is closely related with the theory of blood vessels.

[Protective effect of electroacupuncture of "xiusanzhen" on neuronal mitochondria in rats with acute cerebral ischemia-reperfusion injury].

Objective: To explore the protective effect of electroacupuncture (EA) of "Xiusanzhen"([Chinese characters: see text]) on mitochondria of cortical neurons in acute cerebral ischemia-reperfusion (CI/R) rats.

Methods: A total of 30 cases of SD rats were divided into control, model and EA groups. CI/R model was established by occlusion of the middle cerebral artery and reperfusion.

[Observation on therapeutic effect of puncturing with needles in alignment at the hairline region on vascular dementia].

Objective: To search for an effective therapy for vascular dementia (VD).

Methods: One hundred and twenty cases of VD were randomly divided into 2 groups, 60 cases in each group. The observation group were treated with puncturing with needles in alignment at the hairline region and the control group with oral administration of Duxil.