The Effectiveness and Safety of Extracts in Rheumatoid Arthritis: A Systematic Review and Meta-Analysis.

To conduct a meta-analysis of the effectiveness and safety of (TwHF) extracts for the treatment of rheumatoid arthritis (RA). A systematic literature search was conducted in PubMed, EMBASE, Cochrane, Medline, CNKI, SinoMed and WanFang Library till 12 July 2017. All included studies were analyzed with the use of the Review Manager 5.

MicroRNA-181a, -146a and -146b in spleen CD4+ T lymphocytes play proinflammatory roles in a murine model of asthma.

CD4+ T lymphocytes can be primarily polarized to differentiate into Th2 cells, and are heavily involved in the Th2 inflammation of asthma. Little is known about the correlation between microRNAs and Th2 inflammation in asthma, therefore we explore the roles of five microRNAs (microRNA-181a, -155, -150, -146a and -146b) in Th2 inflammation of asthma by tracking their expression levels in splenic CD4+ T lymphocytes under different conditions. Using quantitative real-time polymerase chain reaction (qPCR), the dynamic changes of these microRNAs in murine models of acute asthma (i.

[Effect of STAT5 gene silencing on the proliferation of T lymphocytes in a mouse model of asthma].

Objective: To study the effect of silencing STAT5 gene on the proliferation of T lymphocytes in a mouse model of asthma.

Methods: Spleen T-lymphocytes of normal and asthmatic mice were selected by immunomagnetic beads, and the STAT5a/b genes of these T-lymphocytes were silenced by siRNA. The mRNA of STAT5a/b was detected by fluorescence quantitative PCR, and the protein by Western blot.