Publications by authors named "Wen-Wen Gu"

Purpose: Recurrent miscarriage (RM) is a distressing and complicated adverse pregnancy outcome. It is commonly recognized that insufficient decidualization could result in RM, but the molecular mechanisms of decidual impairment are still not fully understood. Thus, this study aimed to identify novel key genes potentially involved in RM and explore their roles played in endometrial decidualization.

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Recurrent miscarriage (RM) is currently difficult to prevent and treat due to a lack of comprehensive understanding of its molecular mechanisms. The aim of this study was to identify genes potentially involved in the pathogenesis of RM and to observe their expression in the decidual tissues of RM patients. A total of 1823 differentially expressed genes (DEGs) and 148 differentially expressed proteins (DEPs) in decidual tissues between RM and control groups were identified.

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In Brief: Insufficiency of extravillous trophoblast (EVT) cell invasion is implicated in pregnancy complications. This study reveals the roles of the miR-486-5p/Smad2 pathway in lipopolysaccharide (LPS)-induced EVT dysfunctions and in the pathogenesis of early pregnancy loss (EPL).

Abstract: Placenta-associated pathologies, including EPL and preeclampsia, are characterized by insufficient EVT invasion.

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Abnormally elevated tumor necrosis factor-α (TNFα) levels at the maternal-fetal interface can lead to adverse pregnancy outcomes, including recurrent miscarriage (RM), but the mechanism underlying upregulated TNFα expression is not fully understood. We previously reported that the interaction between monoclonal nonspecific suppressor factor-β (MNSFβ) and RC3H1 upregulates TNFα expression, but the precise mechanisms are unknown. In this study, we found that MNSFβ stimulated the LPS-induced TNFα expression by inactivating the promoting effect of RC3H1 on TNFα mRNA degradation rather than directly inhibiting the expression of RC3H1 in THP1-Mϕs.

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In clinical practice, the anatomical classification of pulmonary veins plays a crucial role in the preoperative assessment of atrial fibrillation radiofrequency ablation surgery. Accurate classification of pulmonary vein anatomy assists physicians in selecting appropriate mapping electrodes and avoids causing pulmonary arterial hypertension. Due to the diverse and subtly different anatomical classifications of pulmonary veins, as well as the imbalance in data distribution, deep learning models often exhibit poor expression capability in extracting deep features, leading to misjudgments and affecting classification accuracy.

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In the domain of medical image segmentation, traditional diffusion probabilistic models are hindered by local inductive biases stemming from convolutional operations, constraining their ability to model long-term dependencies and leading to inaccurate mask generation. Conversely, Transformer offers a remedy by obviating the local inductive biases inherent in convolutional operations, thereby enhancing segmentation precision. Currently, the integration of Transformer and convolution operations mainly occurs in two forms: nesting and stacking.

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Article Synopsis
  • Non-small cell lung cancer (NSCLC) represents over 80% of lung cancer cases, but its 5-year survival rate remains poor, prompting research into microRNAs like miR-3074-5p as potential regulators of tumor growth.
  • Overexpression of miR-3074-5p in NSCLC cells led to reduced cell proliferation, migration, and invasion while inducing apoptosis, suggesting it plays a critical role in cancer suppression.
  • The study identified YWHAZ as a target of miR-3074-5p and demonstrated that it interacts with the protein Hsp27, implicating the miR-3074-5p/YWHAZ/Hsp27 pathway as a promising therapeutic target for NSCLC
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  • The research on cancer patients returning to work in China is early-stage, highlighting the need for a specialized tool, the Adaptability to Return-to-Work Scale (ARTWS), to measure this group's adaptability.
  • The ARTWS was developed through expert feedback and testing, resulting in a 24-item scale that identifies key factors influencing work adaptability: "Focusing on rehabilitation," "Rebuilding Self-efficiency," and "Adjusting plans," which account for 66.6% of variance.
  • The scale demonstrated strong reliability and validity, making it a valuable resource for assessing cancer patients' readiness to return to work.
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  • * Researchers found that knocking in miR-3074-5p in pregnant mice resulted in higher levels of pro-inflammatory cytokines, more M1 macrophages, and increased embryo resorption compared to wild-type mice.
  • * The findings suggest that miR-3074-5p targets estrogen receptor-α, promoting harmful immune responses and potentially contributing to implantation issues in patients with repeated implantation failure by disrupting the inflammatory balance in early pregnancy.
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Article Synopsis
  • Convolutional Neural Networks (CNNs) are commonly used for medical image segmentation, but they struggle with long-term dependencies due to local inductive biases.
  • The introduction of Transformer technology helps overcome these limitations by allowing for better modeling of long-range relationships, leading to improved segmentation and classification accuracies.
  • The paper presents a parallel hybrid model that combines Transformer and CNN branches to extract both local and global features effectively, achieving high segmentation performance with an average Dice coefficient of 92.65% on the Flare21 dataset and 91.61% on the Amos22 dataset.
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Dysfunction of extravillous trophoblasts (EVTs) might cause early pregnancy failure by interfering with embryo implantation and/or placentation. We previously reported that the villus miR-3074-5p expression level was increased, whereas the peripheral level of GDF15, a predict target gene of miR-3074-5p, was decreased in recurrent miscarriages (RM) patients, and miR-3074-5p could enhance apoptosis but reduce invasion of human extravillous trophoblast cells (EVTs). The aim of this study was to further explore roles of miR-3074-5p/GDF15 pathway in regulation of EVTs function.

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  • Decidual macrophages (dMϕs) are crucial for maintaining a stable environment at the maternal-fetal interface during pregnancy, and problems with their function can lead to issues like recurrent spontaneous abortion (RSA).
  • The study found that lower levels of SEC5 in primary dMϕs from RSA patients lead to impaired M2 polarization and decreased phosphorylation of STAT6, while higher SEC5 expression enhances these processes.
  • SEC5 interacts with STAT6, and reduced SEC5 in macrophages is linked to less effective support for trophoblasts, contributing to early pregnancy loss, as demonstrated in a mouse model.
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The lipopolysaccharide(LPS)-indused RAW264.7 cells inflammation model was used as a carrier to investigated the effects of the preparation quality markers of Yulian Tang with anti-inflammatory activity in vitro. RAW264.

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Decidual macrophages (dMϕ) are the second largest population of leukocytes at the maternal-fetal interface and play critical roles in maintaining pregnancy. Our previous studies demonstrated the active involvement of monoclonal nonspecific suppressor factor-β (MNSFβ) in embryonic implantation and pregnancy success. MNSFβ is a ubiquitously expressed ubiquitin-like protein that also exhibits immune regulatory potential, but its function in human dMϕ remains unknown.

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The invasion of maternal decidua by extravillous trophoblast (EVT) is essential for the establishment and maintenance of pregnancy, and abnormal trophoblast invasion could lead to placenta-associated pathologies including early pregnancy loss and preeclampsia. SEC5, a component of the exocyst complex, plays important roles in cell survival and migration, but its role in early pregnancy has not been reported. Thus, the present study was performed to explore the functions of SEC5 in trophoblast cells.

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Embryo implantation is an essential step for a successful pregnancy, and any defect in this process can lead to a range of pregnancy pathologies. The objective of this study was to explore the role of N-myc downregulated gene 1 (NDRG1) in embryo implantation. It was found that uterine NDRG1 expression has a dynamic pattern during the estrous cycle in nonpregnant mice and that uterine NDRG1 expression was elevated during the implantation process in pregnant mice.

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Background: Implantation failure is not only a major cause of early pregnancy loss, but it is also an obstacle to assisted reproductive technologies. The identification of potential circulating biomarkers for recurrent miscarriage (RM) and/or recurrent implantation failure would contribute to the development of novel diagnosis and prediction techniques.

Methods: MiR (miR-23a-3p, 27a-3p, 29a-3p, 100-5p, 127-3p and 486-5p) expression in the villi, decidual tissues and peripheral blood plasma and serum were validated by qPCR, and the localization of miRs in the villi and decidual tissues of RM and normal pregnancy (NP) women were detected by in situ hybridization.

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Objective: The objective of this study was to observe the effects of the overexpression of miR-3074-5p in human trophoblast cells in vitro.

Design: Experimental in vitro study in HTR8/SVneo cells.

Methods: HTR8/SVneo cells were transfected with miR-3074-5p mimic.

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Purpose: To evaluate the reliability and validity of the EQ-5D in a general population sample in urban China.

Methods: Thousand and eight hundred respondents in 18 communities of Hangzhou, China were recruited by multi-stage stratified random sampling. Respondents self-administered a questionnaire including the EQ-5D, the SF-36, and demographic questions.

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