Publications by authors named "Wen-Si Zhu"
Article Synopsis
- - The study investigates the role of microRNA-214-3p (miR-214-3p) in cardiac fibrosis, particularly in the context of angiotensin II (Ang-II) exposure, revealing decreased levels in fibrotic heart tissue but increased levels in treated myofibroblasts.
- - Administration of miR-214-3p significantly reduced cardiac fibrosis in mice, while in vitro tests showed that miR-214-3p can inhibit collagen expression linked to fibrosis by targeting enhancer of zeste homolog 1 and 2 (EZH1 and EZH2).
- - The research highlights the NF-κB signaling pathway's role in regulating miR-214-3p expression
The role of microRNA-1 (miR-1) in ischemia/reperfusion (I/R)-induced injury is not well illustrated. The present study aimed to investigate the expression and potential target of miR-1 in the myocardium of a rat model of I/R. The apoptosis of cardiomyocytes in the ischemic rat myocardium increased on day 1, then attenuated on day 3 and day 7 post-I/R.
View Article and Find Full Text PDFeRF1aMC and Mg(2+) dependent structure switch of GTP binding to eRF3 in Euplotes octocarinatus.
J Microbiol Biotechnol
February 2012
Eukaryotic translation termination is governed by eRF1 and eRF3. eRF1 recognizes the stop codons and then hydrolyzes peptidyl-tRNA. eRF3, which facilitates the termination process, belongs to the GTPase superfamily.
View Article and Find Full Text PDFTwo genes, MGFc (40) and MGFc (24), encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatography using His60 Ni Superflow. Recombinant MGFc(24) protein was purified using a glutathione-Sepharose 4B column.
View Article and Find Full Text PDFObjective: To study the effects of water and alcohol extracts of several Chinese herbal medicines and other medicines on alcohol dehydrogenase activity in order to provide enzymology basis on new medicine.
Methods: Water or alcohol extracts of Chinese herbal medicine and other medicine were tested on the effects of alcohol dehydrogenase activity by Valle and Hoch method.
Results: Among them, 8 were found to have the effect of activation on alcohol dehydrogenase.