[Determination of trace vitamin B2 by fluorimetry after aqueous two-phase extraction based on acetone and ethanol].

Small molecular water-miscible organic solvent, such as ethanol, acetone and acetonitrile, can be used to form aqueous two-phase system for extraction of analytes in the presence of salts. Just based on it, the acetone-salt-H2O and the ethanol-salt-H2O systems were developed for the determination of trace vitamin B2 coupled with fluorimetry after aqueous two-phase extraction. Various factors including the type and amount of salts and organic solvents, extraction time, coexistent substance, and pH were studied.

[Comparison of non-protected fluid room temperature phosphorescence and micellar-stabilized room temperature phosphorescence properties of indole-3-butyric acid].

For indole-3-butyric acid (IBA), the non-protected fluid room temperature phosphorescence (NP-RTP) and the fluid room temperature phosphorescence with polymeric dispersant polyethyleneglycol-200 (PEG-200), PEG-400 or non-ionic surfactant Tween-20, Tween-40, Tween-80, Tween-85, Brij35 and emulsifier OP as a medium have been studied in detail. When the surfactants or polymeric dispersants were added to the NP-RTP system, the profile of RTP spectrum was not changed, but the RTP intensity and the pre-irradiation time required to attain a stable RTP signal were increased. Whether the surfactants or polymeric dispersants were added to the system or not, no RTP signal was observed when TlNO3 was used as a heavy atom perturber (HAP), but intense RTP emissions were observed when KI was present as HAP.

Studies on properties and application of non-protected room temperature phosphorescence of propranolol.

A direct and simple non-protected room temperature phosphorimetry (NP-RTP) for determine propranolol, which using I- as a heavy atom perturber and sodium sulfite as a deoxygenator, has been developed. The phosphorescence peak wavelength maxima lambda(ex)/lambda(em) = 288/494, 522 nm. The analytical curve of propranolol gives a linear dynamic range of 8.