Evaluation of renal clinicopathological changes in IgA nephropathy by urinary podocytes excretion and podocalyxin expression.

Objective: To explore the association of urinary podocyte excretion and renal expression of podocyte-specific marker podocalyxin (PCX) with clinicopathological changes in immunoglobulin A nephropathy (IgAN).

Methods: Morning urine samples from IgAN patients and healthy controls were collected. The expression of glomerular PCX was quantified in 50 IgAN patients diagnosed by renal biopsy.

Molecular characterization of a Chinese pedigree with beta-thalassemia intermedia.

Hereditary persistence of fetal hemoglobin (HPFH), often associated with mutations in the beta-globin gene cluster, is normally benign, but a person carrying both HPFH and another beta-thalassemia (beta-thal) mutation will develop serious anemia. These people might be erroneously diagnosed as having homozygous beta-thal with common reverse dot-blot methods. Here we report a 5-year old boy with thalassemia intermedia, who is a compound heterozygote for the rare HPFH-6 deletion with codons 41/42 (-TCTT) beta(0)-thal, who inherited the deletion from his mother and the beta(41/42) mutation from his father.

[Establishment of a rat model of mycoplasma pneumonia and the treatment].

Objective: To establish a rat model of mycoplasma pneumonia (MP) for investigating the pathogenesis of MP and its therapy with drugs.

Methods: Thirty Wistar rats were randomly divided into 6 groups (n=5), including a control group, a MP model group, a erythromycin lactobionate group and 3 erythromycin microspheres groups (high, middle, and low dose groups). With the exception of those in the control group, all the rats received intranasal MP administration followed by corresponding treatments administered via tail vein injection.

[Chronic toxicity of 97% isopropyl thioxanthone in rat by oral administration for 2 years].

Objective: To study the oral chronic toxicity of 97% isopropyl thioxanthone (97% ITX) in rats, determine the no-observed adverse effect levels (NOAEL).

Methods: Four groups of rats were fed with foodstuff containing 97% ITX in the dosage of 1000.0, 250.

[Antimicrobial effects of erythromycin microspheres against Mycoplasma Pneumoniae in rats].

Objective: To evaluate the antimicrobial effects of erythromycin microspheres against Mycoplasma pneumoniae in rats.

Methods: With erythromycin lactobionate as the positive control, erythromycin microspheres at 3 non-toxic doses (0.1, 0.

[Rapid detection of oxacillin and erythromycin resistance genes in Staphylococcus aureus using multiplex PCR].

Objective: To establish a rapid multiplex PCR (MPCR) detection system of oxacillin and erythromycin resistance genes in Staphylococcus aureus (S. aureus) and evaluate the genotype distribution of the genes associated to mecA, ermA and ermC resistance in Guangzhou.

Methods: The S.

[Nephrotoxicity of high- and low-osmolar contrast media: Protective role of fosinopril or telmisartan in a rat model].

Objective: To compare the nephrotoxicity of high- and low-osmolar contrast media (HOCM and LOCM), and to determine the protective role of fosinopril or telmisartan and its possible mechanism.

Methods: Forty eight healthy SD rats were randomly divided into 6 groups: a normal control group, a glycerol control group, a low-osmolar contrast media (LOCM) group, a high-osmolar contrast media (HOCM) group, a fosinopril group, and a telmisartan group. Glycerine for inducing kidney damage was given to all rats except the normal control group.

[Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis].

Objective: To identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization.

Methods: Recombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.

[Induced differentiation and signaling factor PTEN expression of 3T3-L1 adipocytes].

Objective: To optimize the condition for inducing the differentiation of 3T3-L1 preadipocytes into adipocytes and study the expression of PTEN tumor suppression gene in this process, aiming to understand the regulatory role of PTEN in normal adipocyte differentiation and collect laboratory evidence for developing drugs targeting PTEN.

Methods: The differentiation of 3T3-L1 preadipocytes cultured in high-glucose DMEM were induced according to 2 protocols with different combinations of dexamethasone, isobutylmethylxanthine (IBMX) and insulin, and the resultant adipocytes were identified by oil red O staining. The total proteins of 3T3-L1 were extracted and analyzed by Western blotting, and PTEN homology between mice and human was analyzed by bioinformatic method.

[Effects of Alternanthera philoxeroides Griseb against respiratory syncytial virus infection in mice].

Objective: To investigate the effect of an oral preparation of Alternathera philoxeroides Griseb (APG) against respiratory syncytical virus (RSV) in mice.

Methods: APG preparation was administered orally in RSV-infected mice at different daily doses (2.5, 4.

[Investigation of viral pathogens contributing to habitual abortion].

Objective: To investigate the association of Coxsackie B virus (CBV) with habitual abortion.

Methods: CBV IgM antibody, viral RNA and virions were detected in 86 women with habitual abortion and 40 with induced abortion by means of enzyme-linked immunosorbent assay (ELISA), RT-PCR and virus isolation, respectively.

Results: The positivity rate of CBV IgM were 87.

[Effects of Alternanthera philoxeroides Griseb against dengue virus in vitro].

Objective: To investigate the effects of Alternanthera philoxeroides Griseb extracts against dengue virus in vitro.

Methods: MTT assay and observation of cytopathic effect (CPE) were carried out to determine the cytotoxicity of Alternanthera philoxeroides Griseb on C6/36 cell lines and its effects on dengue virus.

Results: None of the 4 kinds of Alternanthera philoxeroides Griseb extracts exhibited obvious cytotoxicity on the cells at different concentrations with the exception of that over 320 microg/ml.

Detection of serum Chlamydia pneumoniae (Cpn) immune complex and Cpn antibody in patients with coronary heart disease.

Objective: To understand the state of Chlamydia pneumoniae (Cpn) infection in patients with coronary heart disease (CHD), and explore the relationship between Cpn infection and the gonesis and progressin of CHD.

Method: By adopting sandwich method with double antibodies and indirect ELISA method, we detected serum Cpn immune complex and IgG antibody in 160 CHD patients (confirmed by coronary artery angiography) and 40 subjects without CHD.

Results: The positivity rate of Cpn IgG type immune complex in the CHD group (63.

[Relationship between cyclins and prognosis of acute leukemia].

Background & Objective: Cell proliferation and differentiation are directed by cell cycle mechanism. When tumor cells proliferate abnormally, cyclins, which are positive agents of cell cycle, may be expressed abnormally at the same time. Now many references proved that cyclins are highly expressed in solid tumors.