Characterization of trimethylamine metabolic pathways using pseudo-targeted metabolomics.

Trimethylamine (TMA) metabolism comprises choline-containing compounds' metabolization, TMA production and trimethylamine N-oxide (TMAO) generation. However, the presence of numerous compounds in the carnitine and phosphatidylcholine (PC) pool compositions complicates profiling work significantly. This study is aimed at developing an efficient method for profiling TMA metabolic pathways, including quantifying known compounds and semi-quantifying the differential metabolites in the carnitine and PC pool compositions.

[Studies on dynamic variation of six isoflavones contents in rhizomes of Belamcanda chinensis].

Objective: To study the monthly dynamic variation of the contents of six isoflavones in the rhizomes of Belamcanda chinensis cultivated in Nanjing, and decide optimum harvesting times.

Method: HPLC method was used to determine the contents of six isoflavones in the samples collected at different times.

Result: It was shown that the total contents of six isoflavones in the rhizomes of B.