Publications by authors named "Wen-Jui Yang"
Purpose: The purposes of this study were to determine whether biomechanical properties of mature oocytes could predict usable blastocyst formation better than morphological information or maternal factors, and to demonstrate the safety of the aspiration measurement procedure used to determine the biomechanical properties of oocytes.
Methods: A prospective split cohort study was conducted with patients from two IVF clinics who underwent in vitro fertilization. Each patient's oocytes were randomly divided into a measurement group and a control group.
Despite serum progesterone being a widely accepted method for luteal phase support during embryo transfer cycles, debates persist regarding the optimal strategy for guiding clinical decisions on progesterone dosages to maximize reproductive outcomes. This retrospective study explored the utility of microRNA (miRNA) biomarkers in guiding personalized progesterone dosage adjustments for frozen embryo transfer (FET) cycles in 22 in vitro fertilization (IVF) patients undergoing hormone replacement therapy. Utilizing MIRA, an miRNA-based endometrial receptivity test, we analyzed patients' miRNA expression profiles before and after progesterone dosage adjustments to determine suitable dosages and assess endometrial status.
View Article and Find Full Text PDFArticle Synopsis
- Advances in IVF have not completely resolved embryo implantation failures, with one key issue being the "displaced window of implantation" (WOI), where the timing of embryo transfer does not sync with the uterine environment.
- The study aims to create a miRNA-based classifier to pinpoint the optimal timing for embryo transfer by analyzing miRNA expression profiles from 200 IVF patients.
- The developed classifier showed high accuracy (93.9% in the training set and 88.5% in the testing set) in identifying the correct WOI, indicating its potential to improve IVF outcomes.
Article Synopsis
- * Recent research highlights that cell-free miRNAs from blood samples can be a non-invasive way to analyze endometrial receptivity, potentially replacing traditional methods.
- * In this study, 111 blood samples were examined using advanced sequencing, achieving a high accuracy of 95.9% in predicting endometrial receptivity status, with significant differences in miRNA expression noted between various receptivity stages.
To evaluate the dynamic expression profiling alterations of SARS-CoV-2-associated molecules within the fertile human endometrium throughout the menstrual cycle. Furthermore, to explore the inherent vulnerability of the endometrium to SARS-CoV-2 infection among women experiencing recurrent pregnancy failure, including both recurrent implantation failures (RIF) and recurrent pregnancy losses (RPL). The present study employed multiple datasets to investigate the expression patterns of SARS-CoV-2-associated genes.
View Article and Find Full Text PDFPurpose: Investigate whether local angiotensin II (AngII) and its AngII type 1 and 2 receptors (AT1R, AT2R) in the endometrium are different and correlate with microvessel density in women with reproductive failure and pregnancy outcomes.
Methods: Endometrium during the window of implantation from 40 women with recurrent miscarriage (RM) and 40 with recurrent implantation failure (RIF) were compared with 27 fertile women. Peri-implantation endometrium from 54 women prior to euploid embryo transfer were collected and compared in women with successful pregnancy and unsuccessful pregnancy.
Article Synopsis
- The study aimed to identify specific microRNAs (miRNAs) that may predict repeated implantation failure (RIF) in patients undergoing in vitro fertilization (IVF).
- Researchers analyzed miRNA profiles from the endometrial tissue of 25 patients in a discovery cohort and validated findings in 11 additional patients.
- They identified three key miRNAs (hsa-miR-20b-5p, hsa-miR-155-5p, hsa-miR-718) that predicted RIF with over 90% accuracy, suggesting these could serve as useful diagnostic biomarkers to improve IVF success rates.*
Problem: A reference range for uterine natural killer (uNK) cell density in the peri-implantation period has recently been established in natural cycles. However, it is uncertain whether the results can be applied to hormonal replacement therapy (HRT) cycles, used increasingly in frozen-thaw embryo replacement cycles and which is known to be capable of supporting implantation.
Method Of Study: A total of 183 women from two IVF centers participated in this study, including 75 women in natural cycles and 108 women in HRT cycles.
Background: To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles.
Methods: The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay.
Background: Single-incision laparoscopic cholecystectomy (SILC) has been shown to be safe for uncomplicated gallbladder diseases. Routinely applying SILC is debatable.
Methods: Two hundred SILCs were performed with single-incision multiple-port longitudinal-array and self-camera techniques.
Purpose: The aim of this study was to evaluate the correlation between embryonic early-cleavage status and the age of patients receiving either a GnRH agonist long protocol or a GnRH antagonist protocol.
Methods: This retrospective study included 534 patients undergoing a fresh cycle of oocyte retrieval and day-3 embryo transfer. Of the 534 patients treated, 331 received a GnRH agonist long stimulation protocol (GnRH agonist group) for ovarian stimulation and 203 patients received a GnRH antagonist protocol (GnRH antagonist group).
Background: To test if early-cleavage was a strong predictor of pregnancy in patients receiving either a GnRH agonist long protocol or a GnRH antagonist protocol for in-vitro fertilization treatment (IVF) and intracytoplasmic sperm injection (ICSI).
Methods: This retrospective study included 534 patients undergoing a fresh cycle of oocyte retrieval and the day-3 embryo transfer (from 22 to 46 years old). Of the 534 patients treated, 331 received a GnRH agonist long stimulation protocol (GnRH agonist group) for ovarian stimulation and 203 patients received a GnRH antagonist protocol (GnRH antagonist group).
Objective: This study was designed to assess the change in uterine position between mock and real embryo transfers.
Materials And Methods: A total of 386 embryo transfer cycles were reviewed, and the uterine position was recorded at the time of mock embryo transfer and then again at the time of real embryo transfer.
Results: Of 254 patients with an anteverted uterus at mock transfer, only 3 (1.
Objective: To compare the early-cleavage rates and the implantation potential of embryos between the GnRH antagonist and agonist long stimulation protocols in women older than 35 years.
Design: Retrospective analysis.
Setting: Academic medical center.