The complete mitochondrial DNA sequence of the cape hare Lepus capensis pamirensis.

Lepus capensis pamirensis is a specific cape hare species that is only distributed in the Pamirs, with a cruel natural environment. The complete mitochondrial genome of the cape hare L. c.

[Genetic structure and subspecies divergence of Lepus capensis in Xinjiang].

To date, the genetic structure and genetic diversity of Lepus capensis in Xinjiang has not been systematically studied at the molecular level, and its subspecies taxonomic status has been under debate for years. According to traditional morphology, there are three subspecies of L. capensis distributed in Xinjiang: L.

[Comparison of statistical methods for detecting differential expression in microarray data].

DNA microarray is a new tool in biotechnology, which allows simultaneously monitoring thousands of gene expression in cells. The goal of differential gene expression analysis is to detect genes with significant change of gene expression levels arising from experimental conditions. Although various statistical methods have been suggested to confirm differential gene expression, only a few studies compared performance of the statistical methods.

[Using single primer PCR to amplify the orf25 gene fragment related to plant male-sterility in Salicornia europaea].

The gene orf25 encodes functional protein that may play an important role in plant fertility control in nature. To clone the orf25 from Salicornia europaea Xinjiang into a T-vector, a single designed primer was used to amplify 1.7kb cDNA fragment with RT-PCR.

[Expression of FMDV VP1 protein in Pichia pastoris and its immunological activity in mice].

Aim: To express and identify bovine O type foot and mouth disease virus protein 1 (FMDV VP1) in yeast Pichia pastoris.

Methods: FMDV vp1 gene was cloned into secretory Pichia pastoris expression vector-pSuperY. After being linearized with enzyme digestion, the vector was transformed into Pichia pastoris SMD1168H by electroporation.