METTL3 contributes to slow transit constipation by regulating miR-30b-5p/PIK3R2/Akt/mTOR signaling cascade through DGCR8.

Background: N6-methyladenosine (m6A) is the most prevalent methylation modification of eukaryotic RNA, and methyltransferase-like 3 (METTL3) plays a vital role in multiple cell functions. This study aimed to investigate the role of m6A methylase METTL3 in slow transit constipation (STC).

Material And Method: The expression of METTL3 and DGCR8 was measured in STC tissues and glutamic acid-induced interstitial cells of Cajal (ICCs).

Microbial reduction of Cr(VI) in the presence of Ni, Cu and Zn by bacterial consortium enriched from an electroplating contaminated site.

The bioremediation of Cr(VI) has been intensively reported in recent years, while little information about Cr(VI)-reducing consortium enriched from in-situ contaminated soil has been revealed, specifically the functional genes involved. In this study, we verified a Cr(VI) reduction process by a consortium enriched from in-situ contaminated soil through enzymatic analysis. The chromate reductase gene ChrR has been successfully amplified and further analyzed, provided solid evidence to prove the Cr(VI) bio-reduction was an enzyme-mediated process.

How the Soil Microbial Communities and Activities Respond to Long-Term Heavy Metal Contamination in Electroplating Contaminated Site.

The effects of long-term heavy metal contamination on the soil biological processes and soil microbial communities were investigated in a typical electroplating site in Zhangjiakou, China. It was found that the soil of the electroplating plant at Zhangjiakou were heavily polluted by Cr, Cr (VI), Ni, Cu, and Zn, with concentrations ranged from 112.8 to 9727.

LncRNA AWPPH and miRNA-21 regulates cancer cell proliferation and chemosensitivity in triple-negative breast cancer by interacting with each other.

Long-non-coding RNAs (lncRNA) AWPPH promotes the progression of liver and bladder cancer, indicating its oncogenic role. The current study aimed to explore the involvement of AWPPH in triple-negative breast cancer (TNBC). In the current study, we found that plasma levels of lncRNA AWPPH and microRNA-21 (miRNA-21) were upregulated in patients with TNBC than in healthy controls, and the upregulation of plasma lncRNA AWPPH and miRNA-21 distinguished early-stage patients with TNBC from healthy controls.

Effects of the long-term consumption of hydrogen-rich water on the antioxidant activity and the gut flora in female juvenile soccer players from Suzhou, China.

Expending a considerable amount of physical energy inevitably leads to fatigue during both training and competition in football. An increasing number of experimental findings have confirmed the relationship between the generation and clearance of free radicals, fatigue, and exercise injury. Recently, hydrogen was identified as a new selective antioxidant with potential beneficial applications in sports.

The occurrence and spatial-temporal distribution of tetrabromobisphenol A in the coastal intertidal zone of Qingdao in China, with a focus on toxicity assessment by biological monitoring.

Tetrabromobisphenol A (TBBPA) is a widely used flame retardant that has increasingly been found as contaminant in aquatic environments. The main goal of this study was to evaluate the pollution level of TBBPA at six locations around Qingdao and assess its biotoxicity through a two-generation toxicity study looking at a copepod species. In the chemical monitoring, the concentration of TBBPA in seawater samples ranged from nd to 1.

Association between high levels of Notch3 expression and high invasion and poor overall survival rates in pancreatic ductal adenocarcinoma.

Pancreatic ductal adenocarcinoma (PDAC) is a commonly fatal tumour. It is characterized by early metastasis and high mortality. Many patients die as a result of PDAC tumour progression.